Q9KCT8 (NAMA_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH dehydrogenase
EC=1.6.99.1

Alternative name(s):
Xenobiotic reductase

Gene names

Name:	namA
Ordered Locus Names:	BH1481

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reduction of the double bond of an array of alpha, beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes By similarity. HAMAP MF_01614
Catalytic activity	NADPH + acceptor = NADP+ + reduced acceptor. HAMAP MF_01614
Cofactor	FMN By similarity. HAMAP MF_01614
Subunit structure	Homotetramer By similarity. HAMAP MF_01614
Sequence similarities	Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.

Ontologies

Keywords
Ligand	FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH dehydrogenase activity Inferred from electronic annotation. Source: EC pentaerythritol trinitrate reductase activity Inferred from electronic annotation. Source: EC trichloro-p-hydroquinone reductive dehalogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	NADPH dehydrogenase HAMAP MF_01614		PRO_0000216116
Regions
	Nucleotide binding	23 – 27	5	FMN By similarity
Sites
	Binding site	28	1	Substrate By similarity
	Binding site	163	1	Substrate By similarity
	Binding site	166	1	Substrate By similarity
	Binding site	214	1	FMN By similarity
	Binding site	307	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KCT8 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: AFC02D5B5595BEAF	FASTA	338	37,170
	10 20 30 40 50 60 MEHKLFSSYV VKGVTLKNRI VMSPMCMYSS DQKDGKIRPF HISHYESRAA GQVGLIIVEA 70 80 90 100 110 120 TAVTPQGRIS PYDLGIWSDD HISGLTETVE RIHAHGAKAA IQLAHAGRKA ELDGPIIAPS 130 140 150 160 170 180 AISYDKMKTP DAMTEEQISE TIEAFKLGAL RAKKAGFDII EIHGAHGYLI NEFLSPLTNK 190 200 210 220 230 240 RTDAYGGSLE NRYRLLREII SEIQTVWDGP LFVRISAAEY AEGGNELSDF ITLAKWMKKQ 250 260 270 280 290 300 GIDLIDCSSG AVVPAPIPVY PGYQVPLAEA IRHEANIATG AVGLITSGIQ AEEILQNERA 310 320 330 DLIFVARELL RNPYWPREAA LELGTTISGP SQYDRAWL

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB05200.1.
PIR	A83835.
RefSeq	NP_242347.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9KCT8.
SMR	Q9KCT8. Positions 4-337.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000051113; EBBACP00000049752; EBBACG00000051104.
GeneID	891006.
GenomeReviews	Gene locus BH1481 in contig BA000004_GR.
KEGG	bha:BH1481.
NMPDR	fig|272558.1.peg.1481.
PATRIC	18940058. VBIBacHal18977_1542.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000002219.
HOGENOM	HBG583461.
OMA	WVEDGWN.
PhylomeDB	Q9KCT8.
ProtClustDB	PRK13523.
Enzyme and pathway databases
BioCyc	BHAL272558:BH1481-MONOMER.
Family and domain databases
HAMAP	MF_01614. NamA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR023663. NADPH_DH. IPR001155. OxRdtase_FMN_N. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K00540.
Pfam	PF00724. Oxidored_FMN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAMA_BACHD

Accession

Primary (citable) accession number: Q9KCT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program