Reviewed, UniProtKB/Swiss-Prot Q9KCT3 (CYSH_BACHD)

Names and origin

Protein names

Recommended name:
    Phosphoadenosine phosphosulfate reductase
    EC=1.8.4.8
Alternative name(s):
    PAPS reductase, thioredoxin dependent
    PAdoPS reductase
    3'-phosphoadenylylsulfate reductase
    PAPS sulfotransferase

Gene names

Name:	cysH
Ordered Locus Names:	BH1486

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_0000100625

Sequences

	Sequence		Length	Mass (Da)
	Q9KCT3-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 1EC6F64F12A6B81B	FASTA	231	27,156
	10 20 30 40 50 60 MDQSIQVNIN EVNQALERKD SLDVIKWAYK TYGDKLVYAC SMGAEGMVLI DLISKVRPDA 70 80 90 100 110 120 PVIFLDTDFH FSETYELIER VKERYPKLQL KLVKPELTPE EQAETYGDRL WERQPDLCCK 130 140 150 160 170 180 LRKLVPLEKE LAQYDAWMSG LRRDQSPTRT NTQFVNEDRR FGSTKICPLI HWTSEEIWMY 190 200 210 220 230 IELHQLPYND LHDKQYPSIG CEYCTRPVKE GEDERAGRWS NTSKTECGLH Q

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000004 Genomic DNA. Translation: BAB05205.1.
PIR	F83835.
RefSeq	NP_242352.1.
3D structure databases
HSSP	HSSP built from PDB template 1SUR based on UniProtKB P17854.
ModBase	Search...
Genome annotation databases
GeneID	890994.
GenomeReviews	Gene locus BH1486 in contig BA000004_GR.
KEGG	bha:BH1486.
NMPDR	fig|272558.1.peg.1486.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9KCT3.
OMA	Q9KCT3. FEETLAY.
Enzyme and pathway databases
BioCyc	BHAL272558:BH1486-MON.
BRENDA	1.8.4.8. 191865.
Family and domain databases
HAMAP	MF_00063. [Tree]
InterPro	IPR011798. APS_reductase. IPR004511. PAdo_PSO4_Rdtase_CysH. IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR02055. APS_reductase. 1 hit. TIGR00434. cysH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_BACHD

Accession

Primary (citable) accession number: Q9KCT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)