Q9KCR5 (PROA_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	BH1504

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000189691

Sequences

	Sequence		Length	Mass (Da)
	Q9KCR5 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 31502055CD267A4D	FASTA	416	45,423
	10 20 30 40 50 60 MSELIEKAKQ AQQASKQLAI LTTEEKNRAL KQIADQLLVE RTYLIEENQK DIEAGQRAGI 70 80 90 100 110 120 SQTLLDRLLL TDERVQAMAE GVEQVIALDD PIGDQIDEFT RPNGLNIRQV RVPLGVIGMI 130 140 150 160 170 180 YEARPNVTVD ASVLCLKSGN AVLLRGSSSA LHSNKALVSV IHRGLEAANV IPKDAVQLLE 190 200 210 220 230 240 DTSRETAKEM FKLNDYLDVL IPRGGANLIQ SVVKEASVPV LETGVGNCHV YIDESADPEM 250 260 270 280 290 300 AVAIAVNAKT QRPSVCNAAE TILVHSAWAK EHVSKLIEEL RIKEVQIAGD EQIKAVAPFV 310 320 330 340 350 360 KPADESDWGT EYLDLQVAMK IVNSVDEAIA HIDRYGSKHS EAIVSETDAN VRKFLTNVDA 370 380 390 400 410 TTVYHNASTR FTDGFEFGFG AEIGISTQKL HARGPMGLRA LTSSKYVVHG TGQIKK

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB05223.1.
PIR	H83837.
RefSeq	NP_242370.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9KCR5.
SMR	Q9KCR5. Positions 3-416.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000051867; EBBACP00000050506; EBBACG00000051858.
GeneID	890633.
GenomeReviews	Gene locus BH1504 in contig BA000004_GR.
KEGG	bha:BH1504.
NMPDR	fig|272558.1.peg.1504.
PATRIC	18940104. VBIBacHal18977_1565.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000000020.
HOGENOM	HBG318080.
OMA	QYPAACN.
PhylomeDB	Q9KCR5.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	BHAL272558:BH1504-MONOMER.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_BACHD

Accession

Primary (citable) accession number: Q9KCR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program