Reviewed, UniProtKB/Swiss-Prot Q9KCR4 (PROB_BACHD)

Names and origin

Protein names

Recommended name:
    Glutamate 5-kinase
    EC=2.7.2.11
Alternative name(s):
    Gamma-glutamyl kinase
      Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	BH1505

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity HAMAP MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	Glutamate 5-kinase HAMAP MF_00456		PRO_0000109636
Regions
	Domain	276 – 354	79	PUA

Sequences

	Sequence		Length	Mass (Da)
	Q9KCR4-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: C6735B3C3FA97282	FASTA	374	40,422
	10 20 30 40 50 60 MKRQRIVIKI GSSSLTTKQG ALDLEKLEGY VRAIVNLRKA GHEVILISSG AVAAGFSRLG 70 80 90 100 110 120 YPVRPTTMAG KQAAAAVGQA LLMQHYMERF HQHGIIAAQL LLTRKDFQNQ TQYTNAYSAL 130 140 150 160 170 180 MELLKRGALP IINENDSTSI AELTFGDNDM LSALVSGLIH AHTLCILTDV NGLYDANPKT 190 200 210 220 230 240 HPNAKRYQYL PTIPDEMLQM GGEAGSNVGT GGMKSKVAAA QTALSLGVDV FIGKASGEHA 250 260 270 280 290 300 LLDILSGKGD GTYVGNTGDL MEGKTKQWIR LHSPVSGVVR IDQGAERAIM NEGKSLLPAG 310 320 330 340 350 360 VTSVHGTFQA GDVVHIWNEQ NKVIAKGQVT LSSEQLRKVQ GRKSDEAKQL IPLVRDEVVH 370 RDQLVIQEKE LNES

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB05224.1.
PIR	A83838.
RefSeq	NP_242371.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	891011.
GenomeReviews	Gene locus BH1505 in contig BA000004_GR.
KEGG	bha:BH1505.
NMPDR	fig|272558.1.peg.1505.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG507643.
OMA	TDVDRLY.
PhylomeDB	Q9KCR4.
Enzyme and pathway databases
BioCyc	BHAL272558:BH1505-MONOMER.
BRENDA	2.7.2.11. 191865.
Family and domain databases
HAMAP	MF_00456. ProB. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu_5kinase. IPR011529. Glu_5kinase_bact. IPR019797. Glutamate_5-kinase_CS. IPR005715. ProB. IPR002478. PUA. IPR015947. PUA-like. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_BACHD

Accession

Primary (citable) accession number: Q9KCR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)