Q9KCG7 (IPYR_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inorganic pyrophosphatase
EC=3.6.1.1

Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name=PPase

Gene names

Name:	ppa
Ordered Locus Names:	BH1604

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	163 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_00209
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00209.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphate-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 163	163	Inorganic pyrophosphatase HAMAP MF_00209		PRO_0000137477
Sites
	Metal binding	57	1	Magnesium 1 By similarity
	Metal binding	62	1	Magnesium 1 By similarity
	Metal binding	62	1	Magnesium 2 By similarity
	Metal binding	94	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KCG7 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 5A8C57910E59BF6F	FASTA	163	18,347
	10 20 30 40 50 60 MAIEPKVIEA FIEIPTGSQN KYEYDKERGV FKLDRVLFSP MFYPAEYGYI ENTLALDGDP 70 80 90 100 110 120 LDVLVIVSNP TFPGCVIDAK VLGFLNMIDG GEEDQKLIAV PTEDPRFKDV NSLNDLPKHK 130 140 150 160 LDEIAHFFKT YKDLEGKKTE VGAYEDAEAA AKLIDECFAR YKG

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB05323.1.
PIR	D83850.
RefSeq	NP_242470.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9KCG7.
SMR	Q9KCG7. Positions 3-162.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000052109; EBBACP00000050748; EBBACG00000052100.
GeneID	890802.
GenomeReviews	Gene locus BH1604 in contig BA000004_GR.
KEGG	bha:BH1604.
NMPDR	fig|272558.1.peg.1604.
PATRIC	18940316. VBIBacHal18977_1671.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00070000036790.
HOGENOM	HBG529150.
OMA	PNDFNVI.
PhylomeDB	Q9KCG7.
ProtClustDB	CLSK695248.
Enzyme and pathway databases
BioCyc	BHAL272558:BH1604-MONOMER.
Family and domain databases
HAMAP	MF_00209. Inorganic_PPase. [Tree]
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
KO	K01507.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
SUPFAM	SSF50324. Pyrophosphatase. 1 hit.
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_BACHD

Accession

Primary (citable) accession number: Q9KCG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program