Reviewed, UniProtKB/Swiss-Prot Q9KCF0 (DDL_BACHD)

Names and origin

Protein names

Recommended name:
    D-alanine--D-alanine ligase
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetase
    D-Ala-D-Ala ligase

Gene names

Name:	ddl
Synonyms:	ddlA
Ordered Locus Names:	BH1621

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP MF_00047
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00047
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	D-alanine--D-alanine ligase HAMAP MF_00047		PRO_0000177784
Regions
	Domain	99 – 300	202	ATP-grasp
	Nucleotide binding	126 – 181	56	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KCF0-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: C806D03993D3D081	FASTA	305	33,126
	10 20 30 40 50 60 MKIAVLYGGT SAEREVSLSS GKGIMEALKA NGHEVIGIDF HPDQVRDLVD LDVDLVFIGL 70 80 90 100 110 120 HGRLGEDGKV QALLDLLNIP YVGTGVQGSA LAMDKAKAKL FFEKAGIRVA EEVVLHSFTY 130 140 150 160 170 180 DANAFNFTGT YPVVVKPNQE GSTIGLTVAE TEEELLQGIE EAFRHDDTIL IEEFIAGTEV 190 200 210 220 230 240 TVAVLGNKGE ERSLPVVEIV PKNKLYDYES KYAPGMSEHI VPARISEEHT AYVQQAAVRA 250 260 270 280 290 300 HQALGCDVYS RVDFIVPNDG SDPVILKVNT LPGMTPTSLY PDAAKGVGMS YEEMIQTFVN LSLKK

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000004 Genomic DNA. Translation: BAB05340.1.
PIR	E83852.
RefSeq	NP_242487.1.
3D structure databases
HSSP	HSSP built from PDB template 1IOW based on UniProtKB P07862.
ModBase	Search...
Genome annotation databases
GeneID	891998.
GenomeReviews	Gene locus BH1621 in contig BA000004_GR.
KEGG	bha:BH1621.
NMPDR	fig|272558.1.peg.1621.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9KCF0.
OMA	YELLNAR.
Enzyme and pathway databases
BioCyc	BHAL272558:BH1621-MON.
BRENDA	6.3.2.4. 191865.
Family and domain databases
HAMAP	MF_00047. Divergent sequence. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_BACHD

Accession

Primary (citable) accession number: Q9KCF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)