Reviewed, UniProtKB/Swiss-Prot Q9KC78 (SYN_BACHD)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	BH1696

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	Asparaginyl-tRNA synthetase HAMAP MF_00534		PRO_0000176390

Sequences

	Sequence		Length	Mass (Da)
	Q9KC78-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E5C61D1868E902DB	FASTA	430	49,114
	10 20 30 40 50 60 MKTTIAKIGQ YVDQEVTLGA WLANKRSSGK IAFLQLRDGT GFIQGVVVKA EVGDEWFQKA 70 80 90 100 110 120 KNLTQESSLY VTGIVRKDER APSGYELTVT SFDIIHEATD YPITPKEHGT EFLMDHRHLW 130 140 150 160 170 180 IRSRKQHAVL RIRNEIIRAT YEFFHENGFV KVDPPILTGS APEGTTELFH TKYFDEDAFL 190 200 210 220 230 240 SQSGQLYMEA AALAFGRVFS FGPTFRAEKS KTRRHLIEFW MIEPEMAFVE FEESLEIQEN 250 260 270 280 290 300 YVAYIVQSVL KHCAIELKTL GRDTSVLESI QAPFPRISYD DAIKFLHEKG FDDIEWGDDF 310 320 330 340 350 360 GAPHETAIAE HFDKPVFITH YPTSLKPFYM EPDPNRDDVV LCADLIAPEG YGEIIGGSQR 370 380 390 400 410 420 ISDYDLLKKR LEEHDLSLDA YAWYLDLRKY GSVPHSGFGL GLERTVGWIS GAGHVRETIP 430 FPRLLNRLYP

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB05415.1.
PIR	H83861.
RefSeq	NP_242562.1.
3D structure databases
SMR	Q9KC78. Positions 4-430.
ModBase	Search...
Genome annotation databases
GeneID	892086.
GenomeReviews	Gene locus BH1696 in contig BA000004_GR.
KEGG	bha:BH1696.
NMPDR	fig|272558.1.peg.1696.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG745843.
OMA	LQKKRHS.
PhylomeDB	Q9KC78.
Enzyme and pathway databases
BioCyc	BHAL272558:BH1696-MONOMER.
BRENDA	6.1.1.22. 191865.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR016027. NA-bd_OB-fold-like. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_BACHD

Accession

Primary (citable) accession number: Q9KC78

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)