Reviewed, UniProtKB/Swiss-Prot Q9KC32 (DAPA1_BACHD)

Names and origin

Protein names

Recommended name:
    Dihydrodipicolinate synthase 1
      Short name=DHDPS 1
    EC=4.2.1.52

Gene names

Name:	dapA1
Ordered Locus Names:	BH1742

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Dihydrodipicolinate synthase 1 HAMAP MF_00418		PRO_0000103082
Regions
	Region	49 – 50	2	Pyruvate binding By similarity
Sites
	Active site	162	1	Schiff-base intermediate with substrate By similarity
	Binding site	107	1	Pyruvate By similarity
	Site	134	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KC32-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 56D3567076CD8FB5	FASTA	295	31,643
	10 20 30 40 50 60 MNIGRIVTAM VTPFNEQDQV NLEATCQLVN ELIANGSDAL VVGGTTGESP TLTTDEKMAL 70 80 90 100 110 120 FHTVVEAAGG RVPVIAGTGS NNTRASIELS KKVEDLGVDG IMLVTPYYNK PSQEGLFQHF 130 140 150 160 170 180 KAISESTSLP IMLYNIPGRS GVNLDAETTL RLAELRNIVS IKEASGNLDQ IAEIIEHSPA 190 200 210 220 230 240 DFSVYSGDDS LTLPLLSIGG TGVVSVASHI IGNEMQEMVQ LFLSGQVERA AAIHRKLLPL 250 260 270 280 290 MKTLFMTPNP TAVKAALELK GLPVGHVRLP LVPLTAEEQR QLELAINPQF NMFVS

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000004 Genomic DNA. Translation: BAB05461.1.
PIR	F83867.
RefSeq	NP_242608.1.
3D structure databases
HSSP	HSSP built from PDB template 1DHP based on UniProtKB P05640.
ModBase	Search...
Genome annotation databases
GeneID	892642.
GenomeReviews	Gene locus BH1742 in contig BA000004_GR.
KEGG	bha:BH1742.
NMPDR	fig|272558.1.peg.1742.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9KC32.
OMA	QKGLIEH.
Enzyme and pathway databases
BioCyc	BHAL272558:BH1742-MON.
BRENDA	4.2.1.52. 191865.
Family and domain databases
HAMAP	MF_00418. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005263. DapA_synth. IPR002220. DHDPS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PRINTS	PR00146. DHPICSNTHASE.
ProDom	PD001859. DHDPS. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00674. dapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA1_BACHD

Accession

Primary (citable) accession number: Q9KC32

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)