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Q9KBR4

- IABF_BACHD

UniProt

Q9KBR4 - IABF_BACHD

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Protein

Intracellular exo-alpha-(1->5)-L-arabinofuranosidase

Gene
abfA, BH1861
Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes the cleavage of terminal alpha-(1->5)-arabinofuranosyl bonds in different hemicellulosic homopolysaccharides (branched and debranched arabinans) By similarity.

Catalytic activityi

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei28 – 281Substrate By similarity
Binding sitei73 – 731Substrate; via amide nitrogen By similarity
Active sitei174 – 1741Proton donor/acceptor By similarity
Binding sitei245 – 2451Substrate By similarity
Active sitei293 – 2931Nucleophile By similarity
Binding sitei293 – 2931Substrate By similarity
Sitei297 – 2971Important for substrate recognition By similarity
Binding sitei350 – 3501Substrate By similarity
Sitei350 – 3501Important for substrate recognition By similarity

GO - Molecular functioni

  1. alpha-L-arabinofuranosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. arabinan catabolic process Source: UniProtKB-UniPathway
  2. L-arabinose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BioCyciBHAL272558:GJC5-1945-MONOMER.
RETL1328306-WGS:GSTH-6053-MONOMER.
UniPathwayiUPA00667.

Protein family/group databases

CAZyiGH51. Glycoside Hydrolase Family 51.

Names & Taxonomyi

Protein namesi
Recommended name:
Intracellular exo-alpha-(1->5)-L-arabinofuranosidase (EC:3.2.1.55)
Short name:
ABF
Alternative name(s):
Intracellular arabinan exo-alpha-(1->5)-L-arabinosidase
Short name:
Arabinosidase
Gene namesi
Name:abfA
Ordered Locus Names:BH1861
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001258: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 500500Intracellular exo-alpha-(1->5)-L-arabinofuranosidasePRO_0000057699Add
BLAST

Interactioni

Subunit structurei

Homohexamer; trimer of dimers By similarity.

Protein-protein interaction databases

STRINGi272558.BH1861.

Structurei

3D structure databases

ProteinModelPortaliQ9KBR4.
SMRiQ9KBR4. Positions 5-499.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni173 – 1742Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3534.
HOGENOMiHOG000236895.
KOiK01209.
OMAiDEFIAWC.
OrthoDBiEOG6SJJF9.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR010720. Alpha-L-AF_C.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF06964. Alpha-L-AF_C. 1 hit.
[Graphical view]
SMARTiSM00813. Alpha-L-AF_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KBR4-1 [UniParc]FASTAAdd to Basket

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MTLTATMVVD KSFKIGEIDK RIYGSFIEHL GRAVYEGIYE PGHPDGDEQG    50
FRKDVIRLVQ ELQVPLVRYP GGNFVSGYNW EDGVGPVSER PKRLDLAWRT 100
TETNEIGTNE FVDWAKKVGA EVNMAVNLGS RGVDAARNLV EYCNHPSGSY 150
WSDLRISHGY KDPHNIKTWC LGNEMDGPWQ IGQKTAEEYG RVAAEAGKVM 200
KLVDPSIELV ACGSSNSKMA TFADWEATVL DHTYDYVDYI SLHTYYGNRD 250
DDLANYLAQS MDMDEFIRSV IAIADYVKAK KRSKKTIHLS FDEWNVWFHS 300
NEADRQITPW SVAPPLLEDI YTFEDALLVG SMLITLLKHA DRVKIACLAQ 350
LVNVIAPIMT EKGGPAWKQT IFYPYMHASV YGRGVALQAQ ISSPKYDSKD 400
FTDVPYLDAA VVHLEEAEEV TIFAVNKHQT ESLNLQCDMR SFEGYHVLEH 450
IVLEHENMKA TNQGREQVTP HHNGDSAIDQ GRLTANLAKL SWNVIRLGKK 500
Length:500
Mass (Da):56,402
Last modified:October 1, 2000 - v1
Checksum:i802337EEA0F225B5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000004 Genomic DNA. Translation: BAB05580.1.
PIRiE83882.
RefSeqiNP_242727.1. NC_002570.2.

Genome annotation databases

EnsemblBacteriaiBAB05580; BAB05580; BAB05580.
GeneIDi890868.
KEGGibha:BH1861.
PATRICi18940852. VBIBacHal18977_1939.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000004 Genomic DNA. Translation: BAB05580.1 .
PIRi E83882.
RefSeqi NP_242727.1. NC_002570.2.

3D structure databases

ProteinModelPortali Q9KBR4.
SMRi Q9KBR4. Positions 5-499.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272558.BH1861.

Protein family/group databases

CAZyi GH51. Glycoside Hydrolase Family 51.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB05580 ; BAB05580 ; BAB05580 .
GeneIDi 890868.
KEGGi bha:BH1861.
PATRICi 18940852. VBIBacHal18977_1939.

Phylogenomic databases

eggNOGi COG3534.
HOGENOMi HOG000236895.
KOi K01209.
OMAi DEFIAWC.
OrthoDBi EOG6SJJF9.

Enzyme and pathway databases

UniPathwayi UPA00667 .
BioCyci BHAL272558:GJC5-1945-MONOMER.
RETL1328306-WGS:GSTH-6053-MONOMER.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR010720. Alpha-L-AF_C.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF06964. Alpha-L-AF_C. 1 hit.
[Graphical view ]
SMARTi SM00813. Alpha-L-AF_C. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis."
    Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.
    Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Entry informationi

Entry nameiIABF_BACHD
AccessioniPrimary (citable) accession number: Q9KBR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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