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Q9KBR4

- IABF_BACHD

UniProt

Q9KBR4 - IABF_BACHD

Protein

Intracellular exo-alpha-(1->5)-L-arabinofuranosidase

Gene

abfA

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes the cleavage of terminal alpha-(1->5)-arabinofuranosyl bonds in different hemicellulosic homopolysaccharides (branched and debranched arabinans) By similarity.By similarity

    Catalytic activityi

    Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei28 – 281SubstrateBy similarity
    Binding sitei73 – 731Substrate; via amide nitrogenBy similarity
    Active sitei174 – 1741Proton donor/acceptorBy similarity
    Binding sitei245 – 2451SubstrateBy similarity
    Active sitei293 – 2931NucleophileBy similarity
    Binding sitei293 – 2931SubstrateBy similarity
    Sitei297 – 2971Important for substrate recognitionBy similarity
    Binding sitei350 – 3501SubstrateBy similarity
    Sitei350 – 3501Important for substrate recognitionBy similarity

    GO - Molecular functioni

    1. alpha-L-arabinofuranosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. arabinan catabolic process Source: UniProtKB-UniPathway
    2. L-arabinose metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism

    Enzyme and pathway databases

    BioCyciBHAL272558:GJC5-1945-MONOMER.
    RETL1328306-WGS:GSTH-6053-MONOMER.
    UniPathwayiUPA00667.

    Protein family/group databases

    CAZyiGH51. Glycoside Hydrolase Family 51.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Intracellular exo-alpha-(1->5)-L-arabinofuranosidase (EC:3.2.1.55)
    Short name:
    ABF
    Alternative name(s):
    Intracellular arabinan exo-alpha-(1->5)-L-arabinosidase
    Short name:
    Arabinosidase
    Gene namesi
    Name:abfA
    Ordered Locus Names:BH1861
    OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
    Taxonomic identifieri272558 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001258: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 500500Intracellular exo-alpha-(1->5)-L-arabinofuranosidasePRO_0000057699Add
    BLAST

    Interactioni

    Subunit structurei

    Homohexamer; trimer of dimers.By similarity

    Protein-protein interaction databases

    STRINGi272558.BH1861.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9KBR4.
    SMRiQ9KBR4. Positions 5-499.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni173 – 1742Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 51 family.Curated

    Phylogenomic databases

    eggNOGiCOG3534.
    HOGENOMiHOG000236895.
    KOiK01209.
    OMAiDEFIAWC.
    OrthoDBiEOG6SJJF9.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR010720. Alpha-L-AF_C.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF06964. Alpha-L-AF_C. 1 hit.
    [Graphical view]
    SMARTiSM00813. Alpha-L-AF_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9KBR4-1 [UniParc]FASTAAdd to Basket

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    MTLTATMVVD KSFKIGEIDK RIYGSFIEHL GRAVYEGIYE PGHPDGDEQG    50
    FRKDVIRLVQ ELQVPLVRYP GGNFVSGYNW EDGVGPVSER PKRLDLAWRT 100
    TETNEIGTNE FVDWAKKVGA EVNMAVNLGS RGVDAARNLV EYCNHPSGSY 150
    WSDLRISHGY KDPHNIKTWC LGNEMDGPWQ IGQKTAEEYG RVAAEAGKVM 200
    KLVDPSIELV ACGSSNSKMA TFADWEATVL DHTYDYVDYI SLHTYYGNRD 250
    DDLANYLAQS MDMDEFIRSV IAIADYVKAK KRSKKTIHLS FDEWNVWFHS 300
    NEADRQITPW SVAPPLLEDI YTFEDALLVG SMLITLLKHA DRVKIACLAQ 350
    LVNVIAPIMT EKGGPAWKQT IFYPYMHASV YGRGVALQAQ ISSPKYDSKD 400
    FTDVPYLDAA VVHLEEAEEV TIFAVNKHQT ESLNLQCDMR SFEGYHVLEH 450
    IVLEHENMKA TNQGREQVTP HHNGDSAIDQ GRLTANLAKL SWNVIRLGKK 500
    Length:500
    Mass (Da):56,402
    Last modified:October 1, 2000 - v1
    Checksum:i802337EEA0F225B5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000004 Genomic DNA. Translation: BAB05580.1.
    PIRiE83882.
    RefSeqiNP_242727.1. NC_002570.2.

    Genome annotation databases

    EnsemblBacteriaiBAB05580; BAB05580; BAB05580.
    GeneIDi890868.
    KEGGibha:BH1861.
    PATRICi18940852. VBIBacHal18977_1939.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000004 Genomic DNA. Translation: BAB05580.1 .
    PIRi E83882.
    RefSeqi NP_242727.1. NC_002570.2.

    3D structure databases

    ProteinModelPortali Q9KBR4.
    SMRi Q9KBR4. Positions 5-499.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272558.BH1861.

    Protein family/group databases

    CAZyi GH51. Glycoside Hydrolase Family 51.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB05580 ; BAB05580 ; BAB05580 .
    GeneIDi 890868.
    KEGGi bha:BH1861.
    PATRICi 18940852. VBIBacHal18977_1939.

    Phylogenomic databases

    eggNOGi COG3534.
    HOGENOMi HOG000236895.
    KOi K01209.
    OMAi DEFIAWC.
    OrthoDBi EOG6SJJF9.

    Enzyme and pathway databases

    UniPathwayi UPA00667 .
    BioCyci BHAL272558:GJC5-1945-MONOMER.
    RETL1328306-WGS:GSTH-6053-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR010720. Alpha-L-AF_C.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF06964. Alpha-L-AF_C. 1 hit.
    [Graphical view ]
    SMARTi SM00813. Alpha-L-AF_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis."
      Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.
      Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

    Entry informationi

    Entry nameiIABF_BACHD
    AccessioniPrimary (citable) accession number: Q9KBR4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 26, 2002
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3