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Q9KBR4 (IABF_BACHD) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Intracellular exo-alpha-(1->5)-L-arabinofuranosidase

Short name=ABF
EC=3.2.1.55
Alternative name(s):
Intracellular arabinan exo-alpha-(1->5)-L-arabinosidase
Short name=Arabinosidase
Gene names
Name:abfA
Ordered Locus Names:BH1861
OrganismBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]
Taxonomic identifier272558 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length500 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes the cleavage of terminal alpha-(1->5)-arabinofuranosyl bonds in different hemicellulosic homopolysaccharides (branched and debranched arabinans) By similarity.

Catalytic activity

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.

Pathway

Glycan metabolism; L-arabinan degradation.

Subunit structure

Homohexamer; trimer of dimers By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 51 family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Cellular componentCytoplasm
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processL-arabinose metabolic process

Inferred from electronic annotation. Source: InterPro

arabinan catabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionalpha-L-arabinofuranosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 500500Intracellular exo-alpha-(1->5)-L-arabinofuranosidase
PRO_0000057699

Regions

Region173 – 1742Substrate binding By similarity

Sites

Active site1741Proton donor/acceptor By similarity
Active site2931Nucleophile By similarity
Binding site281Substrate By similarity
Binding site731Substrate; via amide nitrogen By similarity
Binding site2451Substrate By similarity
Binding site2931Substrate By similarity
Binding site3501Substrate By similarity
Site2971Important for substrate recognition By similarity
Site3501Important for substrate recognition By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9KBR4 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 802337EEA0F225B5

FASTA50056,402
        10         20         30         40         50         60 
MTLTATMVVD KSFKIGEIDK RIYGSFIEHL GRAVYEGIYE PGHPDGDEQG FRKDVIRLVQ 

        70         80         90        100        110        120 
ELQVPLVRYP GGNFVSGYNW EDGVGPVSER PKRLDLAWRT TETNEIGTNE FVDWAKKVGA 

       130        140        150        160        170        180 
EVNMAVNLGS RGVDAARNLV EYCNHPSGSY WSDLRISHGY KDPHNIKTWC LGNEMDGPWQ 

       190        200        210        220        230        240 
IGQKTAEEYG RVAAEAGKVM KLVDPSIELV ACGSSNSKMA TFADWEATVL DHTYDYVDYI 

       250        260        270        280        290        300 
SLHTYYGNRD DDLANYLAQS MDMDEFIRSV IAIADYVKAK KRSKKTIHLS FDEWNVWFHS 

       310        320        330        340        350        360 
NEADRQITPW SVAPPLLEDI YTFEDALLVG SMLITLLKHA DRVKIACLAQ LVNVIAPIMT 

       370        380        390        400        410        420 
EKGGPAWKQT IFYPYMHASV YGRGVALQAQ ISSPKYDSKD FTDVPYLDAA VVHLEEAEEV 

       430        440        450        460        470        480 
TIFAVNKHQT ESLNLQCDMR SFEGYHVLEH IVLEHENMKA TNQGREQVTP HHNGDSAIDQ 

       490        500 
GRLTANLAKL SWNVIRLGKK 

« Hide

References

[1]"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis."
Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.
Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000004 Genomic DNA. Translation: BAB05580.1.
PIRE83882.
RefSeqNP_242727.1. NC_002570.2.

3D structure databases

ProteinModelPortalQ9KBR4.
SMRQ9KBR4. Positions 5-499.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272558.BH1861.

Protein family/group databases

CAZyGH51. Glycoside Hydrolase Family 51.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB05580; BAB05580; BAB05580.
GeneID890868.
KEGGbha:BH1861.
PATRIC18940852. VBIBacHal18977_1939.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3534.
HOGENOMHOG000236895.
KOK01209.
OMAKTHVESE.
OrthoDBEOG6SJJF9.
ProtClustDBCLSK873151.

Enzyme and pathway databases

BioCycBHAL272558:GJC5-1945-MONOMER.
UniPathwayUPA00667.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR010720. Alpha-L-AF_C.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF06964. Alpha-L-AF_C. 1 hit.
[Graphical view]
SMARTSM00813. Alpha-L-AF_C. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameIABF_BACHD
AccessionPrimary (citable) accession number: Q9KBR4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: October 1, 2000
Last modified: November 13, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries