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Protein

Malate synthase G

Gene

glcB

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei117 – 1171Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei275 – 2751Acetyl-CoAUniRule annotation
Binding sitei312 – 3121Acetyl-CoAUniRule annotation
Active sitei339 – 3391Proton acceptorUniRule annotation
Binding sitei339 – 3391GlyoxylateUniRule annotation
Metal bindingi431 – 4311MagnesiumUniRule annotation
Binding sitei431 – 4311GlyoxylateUniRule annotation
Metal bindingi459 – 4591MagnesiumUniRule annotation
Binding sitei540 – 5401Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei630 – 6301Proton donorUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBHAL272558:GJC5-2217-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:BH2133
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001258 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 727727Malate synthase GPRO_0000166880Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei616 – 6161Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272558.BH2133.

Structurei

3D structure databases

SMRiQ9KB03. Positions 7-724.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 1252Acetyl-CoA bindingUniRule annotation
Regioni456 – 4594Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KB03-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METYEHVGNL QVATKLITFI EQRALPGTGI KKDDFWSGVQ QIITELMPEN
60 70 80 90 100
KMLLAKREEI QATIDAWHQK NKGPIDFSAY HSFLEEIGYL EPIPEHVTIT
110 120 130 140 150
TENVDDEIAA QAGPQLVVPV NNARYAINAA NARWGSLYDA LYGSNVISEE
160 170 180 190 200
DGCEKTGTYN PKRGTKVIQF AKDFLDHTFP LTSGSHHEAI NYAIMDKQLV
210 220 230 240 250
VTLESGKMTR LKDETQFVGY QGSQGDPSVI LLLHHGLHVE IQIDARHPIG
260 270 280 290 300
KSDRAKVKDI VLESALTTIM DCEDSVAAVD AEDKVAVYQN WLGLMKGTLE
310 320 330 340 350
ATFTKEGKTK KRKLNEDRSY TAPNGETFSL PGRSLMFVRN VGHLMTTPVI
360 370 380 390 400
RTQSGEEVPE GILDGIVTSL IAKHDLLQNG TFRNSKKGSV YIVKPKMHGS
410 420 430 440 450
EEVAFANRLF NRIEDILGLE RHTLKIGVMD EERRTSLNLK ACIEKVKERV
460 470 480 490 500
VFINTGFLDR TGDEIHTSME AGPMIRKGDM KSSSWLSAYE RSNVAAGLTC
510 520 530 540 550
GFQGRAQIGK GMWAMPDLMN EMMEQKGTQL EAGANTAWVP SPTAATLHAI
560 570 580 590 600
HYHRHHVPAI QKTLADDQTC YRKEILEIPL AGSTDWTNED IQAELNNNAQ
610 620 630 640 650
GILGYVVRWV EQGIGCSKVP DIHNTALMED RATLRISSQH MANWLRHGIV
660 670 680 690 700
SKEQVIRTME RMAKVVDEQN AGDPAYRPMA DNLEQSVAFQ AALELVLKGT
710 720
EQPSGYTEPI LHRRRLEFKQ KIAKELV
Length:727
Mass (Da):81,208
Last modified:September 30, 2000 - v1
Checksum:iCE18B45930E5EAE5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB05852.1.
PIRiE83916.
RefSeqiNP_242999.1. NC_002570.2.
WP_010898290.1. NC_002570.2.

Genome annotation databases

EnsemblBacteriaiBAB05852; BAB05852; BAB05852.
KEGGibha:BH2133.
PATRICi18941422. VBIBacHal18977_2224.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB05852.1.
PIRiE83916.
RefSeqiNP_242999.1. NC_002570.2.
WP_010898290.1. NC_002570.2.

3D structure databases

SMRiQ9KB03. Positions 7-724.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272558.BH2133.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB05852; BAB05852; BAB05852.
KEGGibha:BH2133.
PATRICi18941422. VBIBacHal18977_2224.

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.
BioCyciBHAL272558:GJC5-2217-MONOMER.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis."
    Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.
    Nucleic Acids Res. 28:4317-4331(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Entry informationi

Entry nameiMASZ_BACHD
AccessioniPrimary (citable) accession number: Q9KB03
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2003
Last sequence update: September 30, 2000
Last modified: March 31, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.