Reviewed, UniProtKB/Swiss-Prot Q9KB03 (MASZ_BACHD)

Names and origin

Protein names

Recommended name:
    Malate synthase G
    EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	BH2133

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	727 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity. HAMAP MF_00641
Subcellular location	Cytoplasm By similarity HAMAP MF_00641.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 727	727	Malate synthase G HAMAP MF_00641		PRO_0000166880
Sites
	Active site	339	1	Proton acceptor By similarity
	Active site	630	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KB03-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: CE18B45930E5EAE5	FASTA	727	81,208
	10 20 30 40 50 60 METYEHVGNL QVATKLITFI EQRALPGTGI KKDDFWSGVQ QIITELMPEN KMLLAKREEI 70 80 90 100 110 120 QATIDAWHQK NKGPIDFSAY HSFLEEIGYL EPIPEHVTIT TENVDDEIAA QAGPQLVVPV 130 140 150 160 170 180 NNARYAINAA NARWGSLYDA LYGSNVISEE DGCEKTGTYN PKRGTKVIQF AKDFLDHTFP 190 200 210 220 230 240 LTSGSHHEAI NYAIMDKQLV VTLESGKMTR LKDETQFVGY QGSQGDPSVI LLLHHGLHVE 250 260 270 280 290 300 IQIDARHPIG KSDRAKVKDI VLESALTTIM DCEDSVAAVD AEDKVAVYQN WLGLMKGTLE 310 320 330 340 350 360 ATFTKEGKTK KRKLNEDRSY TAPNGETFSL PGRSLMFVRN VGHLMTTPVI RTQSGEEVPE 370 380 390 400 410 420 GILDGIVTSL IAKHDLLQNG TFRNSKKGSV YIVKPKMHGS EEVAFANRLF NRIEDILGLE 430 440 450 460 470 480 RHTLKIGVMD EERRTSLNLK ACIEKVKERV VFINTGFLDR TGDEIHTSME AGPMIRKGDM 490 500 510 520 530 540 KSSSWLSAYE RSNVAAGLTC GFQGRAQIGK GMWAMPDLMN EMMEQKGTQL EAGANTAWVP 550 560 570 580 590 600 SPTAATLHAI HYHRHHVPAI QKTLADDQTC YRKEILEIPL AGSTDWTNED IQAELNNNAQ 610 620 630 640 650 660 GILGYVVRWV EQGIGCSKVP DIHNTALMED RATLRISSQH MANWLRHGIV SKEQVIRTME 670 680 690 700 710 720 RMAKVVDEQN AGDPAYRPMA DNLEQSVAFQ AALELVLKGT EQPSGYTEPI LHRRRLEFKQ KIAKELV

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB05852.1.
PIR	E83916.
RefSeq	NP_242999.1.
3D structure databases
SMR	Q9KB03. Positions 7-724.
ModBase	Search...
Genome annotation databases
GeneID	891319.
GenomeReviews	Gene locus BH2133 in contig BA000004_GR.
KEGG	bha:BH2133.
NMPDR	fig|272558.1.peg.2133.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG350005.
OMA	PKMHGPD.
PhylomeDB	Q9KB03.
Enzyme and pathway databases
BioCyc	BHAL272558:BH2133-MONOMER.
BRENDA	2.3.3.9. 191865.
Family and domain databases
HAMAP	MF_00641. Malate_synth_G. [Tree]
InterPro	IPR011076. Malate_synth-like_core. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_BACHD

Accession

Primary (citable) accession number: Q9KB03

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)