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Protein

Malate synthase G

Gene

glcB

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Isocitrate lyase (aceA)
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei117Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei275Acetyl-CoAUniRule annotation1
Binding sitei312Acetyl-CoAUniRule annotation1
Active sitei339Proton acceptorUniRule annotation1
Binding sitei339GlyoxylateUniRule annotation1
Metal bindingi431MagnesiumUniRule annotation1
Binding sitei431GlyoxylateUniRule annotation1
Metal bindingi459MagnesiumUniRule annotation1
Binding sitei540Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei630Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:BH2133
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001258 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001668801 – 727Malate synthase GAdd BLAST727

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei616Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272558.BH2133.

Structurei

3D structure databases

ProteinModelPortaliQ9KB03.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 125Acetyl-CoA bindingUniRule annotation2
Regioni456 – 459Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C82. Bacteria.
COG2225. LUCA.
HOGENOMiHOG000220740.
KOiK01638.
OMAiTEPVLHA.
OrthoDBiPOG091H05P0.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KB03-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METYEHVGNL QVATKLITFI EQRALPGTGI KKDDFWSGVQ QIITELMPEN
60 70 80 90 100
KMLLAKREEI QATIDAWHQK NKGPIDFSAY HSFLEEIGYL EPIPEHVTIT
110 120 130 140 150
TENVDDEIAA QAGPQLVVPV NNARYAINAA NARWGSLYDA LYGSNVISEE
160 170 180 190 200
DGCEKTGTYN PKRGTKVIQF AKDFLDHTFP LTSGSHHEAI NYAIMDKQLV
210 220 230 240 250
VTLESGKMTR LKDETQFVGY QGSQGDPSVI LLLHHGLHVE IQIDARHPIG
260 270 280 290 300
KSDRAKVKDI VLESALTTIM DCEDSVAAVD AEDKVAVYQN WLGLMKGTLE
310 320 330 340 350
ATFTKEGKTK KRKLNEDRSY TAPNGETFSL PGRSLMFVRN VGHLMTTPVI
360 370 380 390 400
RTQSGEEVPE GILDGIVTSL IAKHDLLQNG TFRNSKKGSV YIVKPKMHGS
410 420 430 440 450
EEVAFANRLF NRIEDILGLE RHTLKIGVMD EERRTSLNLK ACIEKVKERV
460 470 480 490 500
VFINTGFLDR TGDEIHTSME AGPMIRKGDM KSSSWLSAYE RSNVAAGLTC
510 520 530 540 550
GFQGRAQIGK GMWAMPDLMN EMMEQKGTQL EAGANTAWVP SPTAATLHAI
560 570 580 590 600
HYHRHHVPAI QKTLADDQTC YRKEILEIPL AGSTDWTNED IQAELNNNAQ
610 620 630 640 650
GILGYVVRWV EQGIGCSKVP DIHNTALMED RATLRISSQH MANWLRHGIV
660 670 680 690 700
SKEQVIRTME RMAKVVDEQN AGDPAYRPMA DNLEQSVAFQ AALELVLKGT
710 720
EQPSGYTEPI LHRRRLEFKQ KIAKELV
Length:727
Mass (Da):81,208
Last modified:October 1, 2000 - v1
Checksum:iCE18B45930E5EAE5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB05852.1.
PIRiE83916.
RefSeqiWP_010898290.1. NC_002570.2.

Genome annotation databases

EnsemblBacteriaiBAB05852; BAB05852; BAB05852.
KEGGibha:BH2133.
PATRICi18941422. VBIBacHal18977_2224.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB05852.1.
PIRiE83916.
RefSeqiWP_010898290.1. NC_002570.2.

3D structure databases

ProteinModelPortaliQ9KB03.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272558.BH2133.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB05852; BAB05852; BAB05852.
KEGGibha:BH2133.
PATRICi18941422. VBIBacHal18977_2224.

Phylogenomic databases

eggNOGiENOG4105C82. Bacteria.
COG2225. LUCA.
HOGENOMiHOG000220740.
KOiK01638.
OMAiTEPVLHA.
OrthoDBiPOG091H05P0.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMASZ_BACHD
AccessioniPrimary (citable) accession number: Q9KB03
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.