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Q9KB03

- MASZ_BACHD

UniProt

Q9KB03 - MASZ_BACHD

Protein

Malate synthase G

Gene

glcB

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei117 – 1171Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei275 – 2751Acetyl-CoAUniRule annotation
    Binding sitei312 – 3121Acetyl-CoAUniRule annotation
    Active sitei339 – 3391Proton acceptorUniRule annotation
    Binding sitei339 – 3391GlyoxylateUniRule annotation
    Metal bindingi431 – 4311MagnesiumUniRule annotation
    Binding sitei431 – 4311GlyoxylateUniRule annotation
    Metal bindingi459 – 4591MagnesiumUniRule annotation
    Binding sitei540 – 5401Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei630 – 6301Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciBHAL272558:GJC5-2217-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:BH2133
    OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
    Taxonomic identifieri272558 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001258: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 727727Malate synthase GPRO_0000166880Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei616 – 6161Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi272558.BH2133.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9KB03.
    SMRiQ9KB03. Positions 7-724.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni124 – 1252Acetyl-CoA bindingUniRule annotation
    Regioni456 – 4594Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiSQFIENE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9KB03-1 [UniParc]FASTAAdd to Basket

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    METYEHVGNL QVATKLITFI EQRALPGTGI KKDDFWSGVQ QIITELMPEN    50
    KMLLAKREEI QATIDAWHQK NKGPIDFSAY HSFLEEIGYL EPIPEHVTIT 100
    TENVDDEIAA QAGPQLVVPV NNARYAINAA NARWGSLYDA LYGSNVISEE 150
    DGCEKTGTYN PKRGTKVIQF AKDFLDHTFP LTSGSHHEAI NYAIMDKQLV 200
    VTLESGKMTR LKDETQFVGY QGSQGDPSVI LLLHHGLHVE IQIDARHPIG 250
    KSDRAKVKDI VLESALTTIM DCEDSVAAVD AEDKVAVYQN WLGLMKGTLE 300
    ATFTKEGKTK KRKLNEDRSY TAPNGETFSL PGRSLMFVRN VGHLMTTPVI 350
    RTQSGEEVPE GILDGIVTSL IAKHDLLQNG TFRNSKKGSV YIVKPKMHGS 400
    EEVAFANRLF NRIEDILGLE RHTLKIGVMD EERRTSLNLK ACIEKVKERV 450
    VFINTGFLDR TGDEIHTSME AGPMIRKGDM KSSSWLSAYE RSNVAAGLTC 500
    GFQGRAQIGK GMWAMPDLMN EMMEQKGTQL EAGANTAWVP SPTAATLHAI 550
    HYHRHHVPAI QKTLADDQTC YRKEILEIPL AGSTDWTNED IQAELNNNAQ 600
    GILGYVVRWV EQGIGCSKVP DIHNTALMED RATLRISSQH MANWLRHGIV 650
    SKEQVIRTME RMAKVVDEQN AGDPAYRPMA DNLEQSVAFQ AALELVLKGT 700
    EQPSGYTEPI LHRRRLEFKQ KIAKELV 727
    Length:727
    Mass (Da):81,208
    Last modified:October 1, 2000 - v1
    Checksum:iCE18B45930E5EAE5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000004 Genomic DNA. Translation: BAB05852.1.
    PIRiE83916.
    RefSeqiNP_242999.1. NC_002570.2.

    Genome annotation databases

    EnsemblBacteriaiBAB05852; BAB05852; BAB05852.
    GeneIDi891319.
    KEGGibha:BH2133.
    PATRICi18941422. VBIBacHal18977_2224.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000004 Genomic DNA. Translation: BAB05852.1 .
    PIRi E83916.
    RefSeqi NP_242999.1. NC_002570.2.

    3D structure databases

    ProteinModelPortali Q9KB03.
    SMRi Q9KB03. Positions 7-724.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272558.BH2133.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB05852 ; BAB05852 ; BAB05852 .
    GeneIDi 891319.
    KEGGi bha:BH2133.
    PATRICi 18941422. VBIBacHal18977_2224.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi SQFIENE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci BHAL272558:GJC5-2217-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis."
      Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.
      Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

    Entry informationi

    Entry nameiMASZ_BACHD
    AccessioniPrimary (citable) accession number: Q9KB03
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3