Q9KAK2 (NADE_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	BH2285

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152158
Regions
	Nucleotide binding	43 – 50	8	ATP By similarity
Sites
	Active site	45	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KAK2 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 963B238DD1F6DB93	FASTA	272	30,261
	10 20 30 40 50 60 MQQQIIKELH VSQMVEPKEE IRKRVTFLKN YLKHSGAKGY VLGLSGGQDS TLAGKLAQMA 70 80 90 100 110 120 IDELNEEEQD TSYVFIAVRL PYGVQKDEAD AQDAIAFIKP SRSITVNIKD AVDASTKSFE 130 140 150 160 170 180 QATGEVLSDF NKGNTKARER MKAQYDVGAH YGCLVIGTDH AAEAITGFFT KHGDGACDVA 190 200 210 220 230 240 PLFGLTKRQG KSLLKELGAP THLYTKAPTA DLEDDRPGLP DEEALGLTYE QLDDYLEGKQ 250 260 270 VHDAIRKKIE SRYLATEHKR QLPVTIFDSW WK

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB06004.1.
PIR	E83935.
RefSeq	NP_243151.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9KAK2.
SMR	Q9KAK2. Positions 1-272.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000050908; EBBACP00000049547; EBBACG00000050899.
GeneID	891668.
GenomeReviews	Gene locus BH2285 in contig BA000004_GR.
KEGG	bha:BH2285.
NMPDR	fig|272558.1.peg.2285.
PATRIC	18941746. VBIBacHal18977_2386.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000001308.
HOGENOM	HBG351567.
OMA	GLNKRRV.
PhylomeDB	Q9KAK2.
ProtClustDB	PRK00768.
Enzyme and pathway databases
BioCyc	BHAL272558:BH2285-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_BACHD

Accession

Primary (citable) accession number: Q9KAK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2001
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program