Reviewed, UniProtKB/Swiss-Prot Q9KAH5 (IOLA_BACHD)

Names and origin

Protein names

Recommended name:
    Methylmalonate semialdehyde dehydrogenase [acylating]
      Short name=MMSA dehydrogenase
      Short name=MMSDH
      Short name=MSDH
    EC=1.2.1.27
Alternative name(s):
    Malonate semialdehyde dehydrogenase [acetylating]
      Short name=MSA dehydrogenase
    EC=1.2.1.18

Gene names

Name:	iolA
Ordered Locus Names:	BH2312

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts malonate semialdehyde (MSA) and methylmalonate semialdehyde (MMSA) into acetyl-CoA and propanoyl-CoA, respectively By similarity.
Catalytic activity	3-oxopropanoate + CoA + NAD(P)+ = acetyl-CoA + CO2 + NAD(P)H. HAMAP MF_01670 2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH. HAMAP MF_01670
Pathway	Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 7/7. HAMAP MF_01670
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the aldehyde dehydrogenase family. IolA subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	inositol catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	malonate-semialdehyde dehydrogenase (acetylating) activity Inferred from electronic annotation. Source: HAMAP methylmalonate-semialdehyde dehydrogenase (acylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 485	485	Methylmalonate semialdehyde dehydrogenase [acylating] HAMAP MF_01670		PRO_0000352328
Regions
	Nucleotide binding	179 – 183	5	NAD By similarity
Sites
	Active site	287	1	Nucleophile By similarity
	Binding site	386	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KAH5-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 62874DF9CB328A5D	FASTA	485	52,936
	10 20 30 40 50 60 MANLATGEKL KNFIGGQWVE SDSGKTEAVP NPATGEILAH VPISNREDLD RAVSVAKEAF 70 80 90 100 110 120 KTWGKTPVPR RARVLFKYQQ LLVENWEELA RLVTLENGKS YKEAYGEVQR GIECVEFAAG 130 140 150 160 170 180 APSLMMGKQL PDIATNIESG MYRYPIGVVG GITPFNFPMM VPCWMFPLAI ACGNTFVLKP 190 200 210 220 230 240 SERTPLLANR LAELFTEAGL PEGVLNIVHG AHDVVNGLLE HPDVKAISFV GSQPVAEYVY 250 260 270 280 290 300 KTASQHGKRV QALAGAKNHS IVMPDADLDG AVNQIVNAAY GSAGERCMAA AVVVAVGEVA 310 320 330 340 350 360 EPLMEKLQKA VNEITIGNGL DDDVFLGPVI RESHKQKTEN YIELGEKEGA TLVRDGRKDN 370 380 390 400 410 420 VSKDGYFLGP TLFDNVTTEM TIWKEEIFAP VLSVVRVESL DEAIQLTNQS EFANGACLYT 430 440 450 460 470 480 TNGSSVRKFR EEIDAGMLGI NLGVPAPMAF FPFSGWKNSF YGDLHANGTD GVEFYTRKKM ITARW

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000004 Genomic DNA. Translation: BAB06031.1.
PIR	H83938.
RefSeq	NP_243178.1.
3D structure databases
HSSP	HSSP built from PDB template 1O9J based on UniProtKB Q28399.
SMR	Q9KAH5. Positions 9-485.
ModBase	Search...
Genome annotation databases
GeneID	892235.
GenomeReviews	Gene locus BH2312 in contig BA000004_GR.
KEGG	bha:BH2312.
NMPDR	fig|272558.1.peg.2312.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9KAH5.
OMA	NEAYGEV.
Enzyme and pathway databases
BioCyc	BHAL272558:BH2312-MONOMER.
Family and domain databases
HAMAP	MF_01670. [Tree]
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR010061. MeMal-semiAld_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF27. MMSDH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01722. MMSDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IOLA_BACHD

Accession

Primary (citable) accession number: Q9KAH5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)