Q9KAG9 (IOLD_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase
Short name=THcHDO hydrolase
EC=3.7.1.n2

Gene names

Name:	iolD
Ordered Locus Names:	BH2318

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	637 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the cleavage of the C1-C2 bond of 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy-glucuronate (5DG) By similarity. HAMAP MF_01669
Catalytic activity	3,5/4-trihydroxycyclohexa-1,2-dione + H2O = 5-deoxy-glucuronic acid. HAMAP MF_01669
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01669 Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 3/7. HAMAP MF_01669
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding NAD Thiamine pyrophosphate
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	inositol catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	hydrolase activity, acting on acid carbon-carbon bonds, in ketonic substances Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 637	637	3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase HAMAP MF_01669		PRO_0000352533
Regions
	Region	441 – 521	81	Thiamine pyrophosphate binding By similarity
Sites
	Metal binding	492	1	Magnesium By similarity
	Metal binding	519	1	Magnesium By similarity
	Binding site	65	1	Thiamine pyrophosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KAG9 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 36718E3E2535569E	FASTA	637	70,729
	10 20 30 40 50 60 METIRLTTAQ ALIKFLNQQY VHSDGEEFPF VEGIFNIFGH GNVLGIGHAL EQDPGHLKVY 70 80 90 100 110 120 QGKNEQGMAH TAIAYSKQML RKKIYAITTS SGPGSANLVT AAATALANQI PILLLPADTY 130 140 150 160 170 180 ATRQPDPVLQ QFEQEQSIAI TTNDALQPVS RYWDRITRPE QLMSSLIRAF EVMTDPAKAG 190 200 210 220 230 240 PATICISQDV EGEAFDYDVR FFEKRVHYID RKLPSERELK GAADLIKKSK RPLLVVGGGA 250 260 270 280 290 300 KYSEAREALI AFSETFNVPL TETQAGKSAV EASFKNNLGG LGITGTLAAN KAAQQADLVI 310 320 330 340 350 360 GIGTRFTDFA TSSKTLFDFE KVKFVNINVS RMQAYKLDAF PVVADAKRTL EALVPMLEGY 370 380 390 400 410 420 KSEYGDDIGQ LKAEWLKERE RLGKVTFDRE RFEPEIKGHF TQEVMNEYAD ALQTEFAQTT 430 440 450 460 470 480 ALLTINETID PDSVIICAAG SLPGDLQRLW HAEVPNTYHL EYGYSCMGYE VSGTLGLKLA 490 500 510 520 530 540 EPNKEVYAIV GDGSFLMLHS ELVTAIQYHK KINVLLFDNS GYGCINNLQM DFGGGSYFCE 550 560 570 580 590 600 FRTADEKILH VDYAKVAEGY GAKSYRVSTV EELKAALEDA KKQECSTLID IKVLPKTMTD 610 620 630 GYDGSWWNLG VSEVSDREGI QKAYELKQEK LTRAKQY

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB06037.1.
PIR	F83939.
RefSeq	NP_243184.1. NC_002570.2.
3D structure databases
HSSP	HSSP built from PDB template 1JSC based on UniProtKB P07342.
ProteinModelPortal	Q9KAG9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000050987; EBBACP00000049626; EBBACG00000050978.
GeneID	892283.
GenomeReviews	Gene locus BH2318 in contig BA000004_GR.
KEGG	bha:BH2318.
NMPDR	fig|272558.1.peg.2318.
PATRIC	18941814. VBIBacHal18977_2420.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00070000032034.
HOGENOM	HBG294332.
ProtClustDB	CLSK2300076.
Enzyme and pathway databases
BioCyc	BHAL272558:BH2318-MONOMER.
Family and domain databases
HAMAP	MF_01669. IolD. [Tree]
InterPro	IPR023757. THcHDO_hydrolase. IPR012000. Thiamin_PyroP_enz_cen_dom. IPR012001. Thiamin_PyroP_enz_TPP-bd_dom. IPR000399. TPP-bd_CS. IPR011766. TPP_enzyme-bd_C. [Graphical view]
KO	K03336.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IOLD_BACHD

Accession

Primary (citable) accession number: Q9KAG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program