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Protein

tRNA dimethylallyltransferase

Gene

miaA

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A).By similarity

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N(6)-dimethylallyladenine(37) in tRNA.

Cofactori

Mg2+By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 17ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferase (EC:2.5.1.75)
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferase
Short name:
DMAPP:tRNA dimethylallyltransferase
Short name:
DMATase
Isopentenyl-diphosphate:tRNA isopentenyltransferase
Short name:
IPP transferase
Short name:
IPPT
Short name:
IPTase
Gene namesi
Name:miaA
Ordered Locus Names:BH2366
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001258 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001638731 – 314tRNA dimethylallyltransferaseAdd BLAST314

Interactioni

Subunit structurei

Monomer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei101Interaction with substrate tRNABy similarity1
Sitei124Interaction with substrate tRNABy similarity1

Protein-protein interaction databases

STRINGi272558.BH2366.

Structurei

Secondary structure

1314
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 9Combined sources6
Helixi16 – 25Combined sources10
Turni26 – 28Combined sources3
Beta strandi29 – 33Combined sources5
Helixi36 – 39Combined sources4
Turni45 – 48Combined sources4
Helixi52 – 55Combined sources4
Beta strandi60 – 64Combined sources5
Helixi74 – 90Combined sources17
Beta strandi94 – 99Combined sources6
Helixi102 – 110Combined sources9
Helixi121 – 133Combined sources13
Helixi136 – 144Combined sources9
Helixi148 – 151Combined sources4
Helixi159 – 171Combined sources13
Beta strandi190 – 198Combined sources9
Helixi201 – 218Combined sources18
Helixi220 – 229Combined sources10
Helixi237 – 239Combined sources3
Turni241 – 243Combined sources3
Helixi244 – 250Combined sources7
Helixi256 – 279Combined sources24
Beta strandi281 – 283Combined sources3
Beta strandi284 – 288Combined sources5
Helixi294 – 314Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QGNX-ray2.40A1-314[»]
3EXAX-ray2.30A/B/C/D1-314[»]
ProteinModelPortaliQ9KAC3.
SMRiQ9KAC3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9KAC3.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni12 – 17Substrate bindingBy similarity6
Regioni35 – 38Interaction with substrate tRNABy similarity4
Regioni160 – 164Interaction with substrate tRNABy similarity5
Regioni239 – 244Interaction with substrate tRNABy similarity6
Regioni272 – 279Interaction with substrate tRNABy similarity8

Sequence similaritiesi

Belongs to the IPP transferase family.Curated

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039995.
KOiK00791.
OMAiSMSGLGY.
OrthoDBiPOG091H00NK.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
TIGRFAMsiTIGR00174. miaA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KAC3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEKLVAIVG PTAVGKTKTS VMLAKRLNGE VISGDSMQVY RGMDIGTAKI
60 70 80 90 100
TAEEMDGVPH HLIDIKDPSE SFSVADFQDL ATPLITEIHE RGRLPFLVGG
110 120 130 140 150
TGLYVNAVIH QFNLGDIRAD EDYRHELEAF VNSYGVQALH DKLSKIDPKA
160 170 180 190 200
AAAIHPNNYR RVIRALEIIK LTGKTVTEQA RHEEETPSPY NLVMIGLTME
210 220 230 240 250
RDVLYDRINR RVDQMVEEGL IDEAKKLYDR GIRDCQSVQA IGYKEMYDYL
260 270 280 290 300
DGNVTLEEAI DTLKRNSRRY AKRQLTWFRN KANVTWFDMT DVDFDKKIME
310
IHNFIAGKLE EKSK
Length:314
Mass (Da):35,763
Last modified:October 1, 2000 - v1
Checksum:iE1027425C1524942
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB06085.1.
PIRiF83945.
RefSeqiWP_010898520.1. NC_002570.2.

Genome annotation databases

EnsemblBacteriaiBAB06085; BAB06085; BAB06085.
KEGGibha:BH2366.
PATRICi18941918. VBIBacHal18977_2472.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB06085.1.
PIRiF83945.
RefSeqiWP_010898520.1. NC_002570.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QGNX-ray2.40A1-314[»]
3EXAX-ray2.30A/B/C/D1-314[»]
ProteinModelPortaliQ9KAC3.
SMRiQ9KAC3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272558.BH2366.

Protocols and materials databases

DNASUi891429.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB06085; BAB06085; BAB06085.
KEGGibha:BH2366.
PATRICi18941918. VBIBacHal18977_2472.

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039995.
KOiK00791.
OMAiSMSGLGY.
OrthoDBiPOG091H00NK.

Miscellaneous databases

EvolutionaryTraceiQ9KAC3.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
TIGRFAMsiTIGR00174. miaA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMIAA_BACHD
AccessioniPrimary (citable) accession number: Q9KAC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.