Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA dimethylallyltransferase

Gene

miaA

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A).By similarity

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N(6)-dimethylallyladenine(37) in tRNA.

Cofactori

Mg2+By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 178ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciBHAL272558:GJC5-2450-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferase (EC:2.5.1.75)
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferase
Short name:
DMAPP:tRNA dimethylallyltransferase
Short name:
DMATase
Isopentenyl-diphosphate:tRNA isopentenyltransferase
Short name:
IPP transferase
Short name:
IPPT
Short name:
IPTase
Gene namesi
Name:miaA
Ordered Locus Names:BH2366
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001258 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314tRNA dimethylallyltransferasePRO_0000163873Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei101 – 1011Interaction with substrate tRNABy similarity
Sitei124 – 1241Interaction with substrate tRNABy similarity

Protein-protein interaction databases

STRINGi272558.BH2366.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 96Combined sources
Helixi16 – 2510Combined sources
Turni26 – 283Combined sources
Beta strandi29 – 335Combined sources
Helixi36 – 394Combined sources
Turni45 – 484Combined sources
Helixi52 – 554Combined sources
Beta strandi60 – 645Combined sources
Helixi74 – 9017Combined sources
Beta strandi94 – 996Combined sources
Helixi102 – 1109Combined sources
Helixi121 – 13313Combined sources
Helixi136 – 1449Combined sources
Helixi148 – 1514Combined sources
Helixi159 – 17113Combined sources
Beta strandi190 – 1989Combined sources
Helixi201 – 21818Combined sources
Helixi220 – 22910Combined sources
Helixi237 – 2393Combined sources
Turni241 – 2433Combined sources
Helixi244 – 2507Combined sources
Helixi256 – 27924Combined sources
Beta strandi281 – 2833Combined sources
Beta strandi284 – 2885Combined sources
Helixi294 – 31421Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QGNX-ray2.40A1-314[»]
3EXAX-ray2.30A/B/C/D1-314[»]
ProteinModelPortaliQ9KAC3.
SMRiQ9KAC3. Positions 2-314.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9KAC3.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni12 – 176Substrate bindingBy similarity
Regioni35 – 384Interaction with substrate tRNABy similarity
Regioni160 – 1645Interaction with substrate tRNABy similarity
Regioni239 – 2446Interaction with substrate tRNABy similarity
Regioni272 – 2798Interaction with substrate tRNABy similarity

Sequence similaritiesi

Belongs to the IPP transferase family.Curated

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039995.
KOiK00791.
OMAiQFNNLYM.
OrthoDBiEOG661HB3.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
TIGRFAMsiTIGR00174. miaA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KAC3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEKLVAIVG PTAVGKTKTS VMLAKRLNGE VISGDSMQVY RGMDIGTAKI
60 70 80 90 100
TAEEMDGVPH HLIDIKDPSE SFSVADFQDL ATPLITEIHE RGRLPFLVGG
110 120 130 140 150
TGLYVNAVIH QFNLGDIRAD EDYRHELEAF VNSYGVQALH DKLSKIDPKA
160 170 180 190 200
AAAIHPNNYR RVIRALEIIK LTGKTVTEQA RHEEETPSPY NLVMIGLTME
210 220 230 240 250
RDVLYDRINR RVDQMVEEGL IDEAKKLYDR GIRDCQSVQA IGYKEMYDYL
260 270 280 290 300
DGNVTLEEAI DTLKRNSRRY AKRQLTWFRN KANVTWFDMT DVDFDKKIME
310
IHNFIAGKLE EKSK
Length:314
Mass (Da):35,763
Last modified:October 1, 2000 - v1
Checksum:iE1027425C1524942
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB06085.1.
PIRiF83945.
RefSeqiWP_010898520.1. NC_002570.2.

Genome annotation databases

EnsemblBacteriaiBAB06085; BAB06085; BAB06085.
KEGGibha:BH2366.
PATRICi18941918. VBIBacHal18977_2472.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB06085.1.
PIRiF83945.
RefSeqiWP_010898520.1. NC_002570.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QGNX-ray2.40A1-314[»]
3EXAX-ray2.30A/B/C/D1-314[»]
ProteinModelPortaliQ9KAC3.
SMRiQ9KAC3. Positions 2-314.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272558.BH2366.

Protocols and materials databases

DNASUi891429.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB06085; BAB06085; BAB06085.
KEGGibha:BH2366.
PATRICi18941918. VBIBacHal18977_2472.

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039995.
KOiK00791.
OMAiQFNNLYM.
OrthoDBiEOG661HB3.

Enzyme and pathway databases

BioCyciBHAL272558:GJC5-2450-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ9KAC3.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
TIGRFAMsiTIGR00174. miaA. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis."
    Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.
    Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.
  2. "Crystal structure of the full-length tRNA isopentenylpyrophosphate transferase (BH2366) from Bacillus halodurans, northeast structural genomics consortium target BHR41."
    Northeast structural genomics consortium (NESG)
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).

Entry informationi

Entry nameiMIAA_BACHD
AccessioniPrimary (citable) accession number: Q9KAC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 1, 2000
Last modified: January 20, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.