Q9KA91 (DAPA2_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrodipicolinate synthase 2
Short name=DHDPS 2
EC=4.2.1.52

Gene names

Name:	dapA2
Synonyms:	dapA
Ordered Locus Names:	BH2399

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Dihydrodipicolinate synthase 2 HAMAP MF_00418		PRO_0000103083
Regions
	Region	49 – 50	2	Pyruvate binding By similarity
Sites
	Active site	162	1	Schiff-base intermediate with substrate By similarity
	Binding site	107	1	Pyruvate By similarity
	Site	134	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KA91 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: BA67B0EC9611B214	FASTA	292	31,478
	10 20 30 40 50 60 MDFGHVLTAM VTPFDAKGNV DLEKVERLVE FLLANGTEGL VVAGTTGESP TLREEEKLAL 70 80 90 100 110 120 FEKVVTVVNK RVPVIAGTGS NNTYVSAQLT KKATALGVDG IMAVTPYYNK PSQEGMYAHF 130 140 150 160 170 180 AAIAEATHLP VMLYNIPSRS VVNLSAETIV KLSYIPNITS LKEANGDLDH MVSVIEQTPD 190 200 210 220 230 240 TFHLYSGDDS LTLPALSIGA KGVVSVASHI IGPEMQQMMM AFRGGDVEHA ANLHRKLLPI 250 260 270 280 290 MKGLFTAPNP TCVKAALQIK GFDTGGVRLP LVPPTSEQRQ HLQFLIEQLK VS

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB06118.1.
PIR	G83949.
RefSeq	NP_243265.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9KA91.
SMR	Q9KA91. Positions 1-290.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000050575; EBBACP00000049214; EBBACG00000050566.
GeneID	892404.
GenomeReviews	Gene locus BH2399 in contig BA000004_GR.
KEGG	bha:BH2399.
NMPDR	fig|272558.1.peg.2399.
PATRIC	18941984. VBIBacHal18977_2505.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000000637.
HOGENOM	HBG358848.
OMA	MSIDINN.
PhylomeDB	Q9KA91.
ProtClustDB	PRK03170.
Enzyme and pathway databases
BioCyc	BHAL272558:BH2399-MONOMER.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR002220. Dihydrodipicolinate_synth-like. IPR020625. Dihydrodipicolinate_synth_AS. IPR020624. Dihydrodipicolinate_synth_CS. IPR005263. Dihydrodipicolinate_synth_DapA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01714.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PIRSF	PIRSF001365. DHDPS. 1 hit.
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. DapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA2_BACHD

Accession

Primary (citable) accession number: Q9KA91

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program