Reviewed, UniProtKB/Swiss-Prot Q9KA71 (SYP_BACHD)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase
      Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	BH2419

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	569 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity.
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 569	569	Prolyl-tRNA synthetase HAMAP MF_01569		PRO_0000248647

Sequences

	Sequence		Length	Mass (Da)
	Q9KA71-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: CC86EE92B2ABDE35	FASTA	569	63,636
	10 20 30 40 50 60 MKQSFFLVPT MREVPADADV VSHQLMLRAG MIRQVAAGIY TYLPLARRVI RKIESIVREE 70 80 90 100 110 120 LDQTGAQEIT MPTLHPAELW QESGRWEKYG DELMRLTDRH NRQFALGPTH EEVITSLIRD 130 140 150 160 170 180 SINSYKKLPL NVYQIQSKFR DERRPRFGLL RGREFIMKDA YSFHTGGESL DEMYKVMYDA 190 200 210 220 230 240 YSRIFRRAGL NVRPVIADSG AIGGKDTHEF MALADVGEDT IAYSDQSDYA ANIEMAAVKV 250 260 270 280 290 300 NYTRPDEPLL ERELVDTGDA KTIRAVAERL SVQEEKIIKS LLVSIDDEWA LILLRGDHEL 310 320 330 340 350 360 NDIKLKHALG ASDIRLATEE EVLEVIGTEV GTIGPVDVKG VKVIADHGIK SIVNGVCGAN 370 380 390 400 410 420 ESNKHFIHVN EERDFTVDTY ADLRFIQEGD PSPDGKGTIR FAQGIEVGQV FKLGTVYSEK 430 440 450 460 470 480 LGATFLDENG KSQPMLMGCY GIGVSRMVAA VIEQHHDEDG IVWPTSVAPF DVHVLALNVK 490 500 510 520 530 540 KEEQMKLADS VYHSLQQAGL DVLLDDRPER AGVKFKDADL IGLPLRVAVG KRADEGYVEV 550 560 KVRKTGEVIE VHADELVPTI QAKLQQLAE

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000004 Genomic DNA. Translation: BAB06138.1.
PIR	C83952.
RefSeq	NP_243285.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	890502.
GenomeReviews	Gene locus BH2419 in contig BA000004_GR.
KEGG	bha:BH2419.
NMPDR	fig|272558.1.peg.2419.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9KA71.
OMA	VVSHQLM.
Enzyme and pathway databases
BioCyc	BHAL272558:BH2419-MON.
BRENDA	6.1.1.15. 191865.
Family and domain databases
HAMAP	MF_01569. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR004500. Pro-tRNA-synth_IIa_bac. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR007214. YbaK/aa-tRNA-synth-assoc-reg. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_BACHD

Accession

Primary (citable) accession number: Q9KA71

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)