Q9KA15 (TRMD_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	BH2479

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060324
Regions
	Region	133 – 138	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	113	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KA15 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 2BDD756F7F3F71AA	FASTA	246	28,023
	10 20 30 40 50 60 MKIDFLTLFP EMFQGVLHSS ILKQAQERGA VSFRVVNFRE YSENKHKKVD DYPYGGGAGM 70 80 90 100 110 120 VLSPQPLFDA VEDLTKKSSS TPRVILMCPQ GETFTQRKAE ELAQAEHLIL LCGHYEGYDE 130 140 150 160 170 180 RIRSYLVTDE LSIGDYVLTG GELGAMVIAD SVTRLLPAVL GNETSAQTDS FSTGLLEYPQ 190 200 210 220 230 240 YTRPADFRGW KVPDVLLSGH HQNIERWRKE QSLKRTLERR PDLLEGRKLT EEEQELLDSI RKQQEK

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB06198.1.
PIR	G83959.
RefSeq	NP_243345.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9KA15.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000050497; EBBACP00000049136; EBBACG00000050488.
GeneID	892450.
GenomeReviews	Gene locus BH2479 in contig BA000004_GR.
KEGG	bha:BH2479.
NMPDR	fig|272558.1.peg.2479.
PATRIC	18942148. VBIBacHal18977_2587.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000001149.
HOGENOM	HBG285805.
OMA	VCGRFEG.
PhylomeDB	Q9KA15.
ProtClustDB	CLSK2749266.
Enzyme and pathway databases
BioCyc	BHAL272558:BH2479-MONOMER.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_BACHD

Accession

Primary (citable) accession number: Q9KA15

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program