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Q9K9W3 (PYRE_BACHD) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10
Gene names
Name:pyrE
Ordered Locus Names:BH2532
OrganismBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]
Taxonomic identifier272558 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length210 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208

Catalytic activity

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208

Cofactor

Magnesium By similarity. HAMAP MF_01208

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208

Subunit structure

Homodimer By similarity. HAMAP MF_01208

Sequence similarities

Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
   Biological processPyrimidine biosynthesis
   LigandMagnesium
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processnucleoside metabolic process

Inferred from electronic annotation. Source: InterPro

pyrimidine nucleotide biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionorotate phosphoribosyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 210210Orotate phosphoribosyltransferase HAMAP MF_01208
PRO_0000110670

Regions

Region120 – 12895-phosphoribose 1-diphosphate binding By similarity

Sites

Binding site9415-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site9815-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site10015-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site1241Orotate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9K9W3 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 6F060FF21CC0DC2E

FASTA21023,217
        10         20         30         40         50         60 
MKETIAQHLL DIEAVTLRPN DPFTWSSGIK SPIYCDNRLT MGYPHIRQSI AEGFEQLIRS 

        70         80         90        100        110        120 
HFPEVTVVAG TATAGIPHAA WVSDRLQAPM VYVRSKSKGH GKQNQIEGKL SSKDRVVIIE 

       130        140        150        160        170        180 
DLISTGGSVI QAADALREAG ADVLGVVAIF TYGLEKGQDQ LEAANLPSYV LTDYPTLLDV 

       190        200        210 
AIRRGDISEE ELQKLQKWRE NPSSQSWMNE

« Hide

References

[1]"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis."
Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.
Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000004 Genomic DNA. Translation: BAB06251.1.
PIRD83966.
RefSeqNP_243398.1. NC_002570.2.

3D structure databases

ProteinModelPortalQ9K9W3.
SMRQ9K9W3. Positions 1-204.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000050764; EBBACP00000049403; EBBACG00000050755.
GeneID891489.
GenomeReviewsGene locus BH2532 in contig BA000004_GR.
KEGGbha:BH2532.
NMPDRfig|272558.1.peg.2532.
PATRIC18942256. VBIBacHal18977_2641.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000002725.
HOGENOMHBG404341.
OMALPMTYVR.
PhylomeDBQ9K9W3.
ProtClustDBPRK00455.

Enzyme and pathway databases

BioCycBHAL272558:BH2532-MONOMER.

Family and domain databases

HAMAPMF_01208. PyrE.
[Tree]
InterProIPR004467. Or_phspho_trans_clade-1.
IPR023031. Orotate_PribosylTferase.
IPR000836. PRibTrfase.
[Graphical view]
KOK00762.
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
TIGRFAMsTIGR00336. PyrE. 1 hit.
PROSITEPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRE_BACHD
AccessionPrimary (citable) accession number: Q9K9W3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: October 1, 2000
Last modified: January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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