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Protein

Carbamoyl-phosphate synthase pyrimidine-specific large chain

Gene

pyrAB

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 4 Mg2+ or Mn2+ ions per subunit.By similarity

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase pyrimidine-specific small chain (pyrAA), Carbamoyl-phosphate synthase pyrimidine-specific large chain (pyrAB)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi284 – 2841Magnesium or manganese 1By similarity
Metal bindingi298 – 2981Magnesium or manganese 1By similarity
Metal bindingi298 – 2981Magnesium or manganese 2By similarity
Metal bindingi300 – 3001Magnesium or manganese 2By similarity
Metal bindingi820 – 8201Magnesium or manganese 3By similarity
Metal bindingi832 – 8321Magnesium or manganese 3By similarity
Metal bindingi832 – 8321Magnesium or manganese 4By similarity
Metal bindingi834 – 8341Magnesium or manganese 4By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi159 – 21658ATPBy similarityAdd
BLAST
Nucleotide bindingi697 – 75458ATPBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBHAL272558:GJC5-2620-MONOMER.
UniPathwayiUPA00070; UER00115.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase pyrimidine-specific large chain (EC:6.3.5.5)
Alternative name(s):
Carbamoyl-phosphate synthetase ammonia chain
Gene namesi
Name:pyrAB
Ordered Locus Names:BH2536
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001258 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10621062Carbamoyl-phosphate synthase pyrimidine-specific large chainPRO_0000144987Add
BLAST

Proteomic databases

PRIDEiQ9K9V9.

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.By similarity

Protein-protein interaction databases

STRINGi272558.BH2536.

Structurei

3D structure databases

ProteinModelPortaliQ9K9V9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini133 – 327195ATP-grasp 1Add
BLAST
Domaini671 – 861191ATP-grasp 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 401401Carboxyphosphate synthetic domainAdd
BLAST
Regioni402 – 546145Oligomerization domainAdd
BLAST
Regioni547 – 929383Carbamoyl phosphate synthetic domainAdd
BLAST
Regioni930 – 1062133Allosteric domainAdd
BLAST

Sequence similaritiesi

Belongs to the CarB family.Curated
Contains 2 ATP-grasp domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105CU6. Bacteria.
COG0458. LUCA.
HOGENOMiHOG000234583.
KOiK01955.
OMAiSTAYMYS.
OrthoDBiPOG091H01IP.

Family and domain databases

Gene3Di1.10.1030.10. 1 hit.
3.30.1490.20. 2 hits.
3.30.470.20. 2 hits.
3.40.50.1380. 1 hit.
3.40.50.20. 2 hits.
HAMAPiMF_01210_A. CPSase_L_chain_A. 1 hit.
MF_01210_B. CPSase_L_chain_B. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006275. CarbamoylP_synth_lsu.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR011607. MGS-like_dom.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PRINTSiPR00098. CPSASE.
SMARTiSM01096. CPSase_L_D3. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF48108. SSF48108. 1 hit.
SSF52335. SSF52335. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
PROSITEiPS50975. ATP_GRASP. 2 hits.
PS00866. CPSASE_1. 2 hits.
PS00867. CPSASE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9K9V9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKREDIKKI LVIGSGPIVI GQAAEFDYAG TQACQALKEE GYEVILVNSN
60 70 80 90 100
PATIMTDTTM ADRVYIEPLT LEFVSRIIRM ERPDGILPTL GGQTGLNMAV
110 120 130 140 150
ELDQAGILKE YNVELLGTKL DSIQQAEDRD LFRALMKELN EPVPDSEIIH
160 170 180 190 200
TLEEAYTFVE RVGYPIIVRP AYTLGGTGGG LVYNEEDLVE IVTSGLKYSP
210 220 230 240 250
VTQCLVEKSI AGFKEIEYEV MRDGKDHAIV VCNMENIDPV GVHTGDSIVV
260 270 280 290 300
APSQTLSDRE YQMLRNSSLK IIRALGIEGG CNVQFALDPD SFQYYIIEVN
310 320 330 340 350
PRVSRSSALA SKATGYPIAK IAAKIAVGYT LDELLNPITQ TTYASFEPAL
360 370 380 390 400
DYVVSKIPRW PFDKFEAANR SLGTQMKATG EVMAIGRNLE ESLLKAVRSL
410 420 430 440 450
EAGVYHLDQP DVNDLDKESL EKKLTKPDDE RLFALGEAIR RGYTIEELWA
460 470 480 490 500
LTKIDRFFLR SFARIIQLET QLKENVGDLE LLKEAKERGF SDMIIADLWG
510 520 530 540 550
TSEQEVYELR MNHGLSPVYK MVDTCAAEFA SATPYFYGTY EEENESERTD
560 570 580 590 600
KKSILVLGSG PIRIGQGIEF DYATVHTVWA IKEAGYEAII VNNNPETVST
610 620 630 640 650
DFSTSDKLYF EPLTVEDVMH IVNLEQPEGV IVQFGGQTAI NLASELAARG
660 670 680 690 700
VKIIGTALED MDRAEDRDKF EQTLVELNIP QPLGDTATSI EEARQIAERI
710 720 730 740 750
GYPVLVRPSY VLGGRAMEIV YKEEELLNYM AHAVKVNPKH PVLIDRYLTG
760 770 780 790 800
KELEVDAISD GENVYIPGIM EHIERAGVHS GDSIAVYPPQ TVPESLKQKL
810 820 830 840 850
IERTIELARG LRIVGLLNIQ FVWHKDDVYV LEVNPRSSRT VPFLSKVTGV
860 870 880 890 900
PMANVATKVM LGKTLPQLGY ETGYHPEAKE VSVKVPVFSF AKLRRVDITL
910 920 930 940 950
GPEMKSTGEV MGRDKTLEKA LYKGLIASGM SIPTHGSVLF TIADKDKQEA
960 970 980 990 1000
ISLAKRFYQI GFSILATEGT AHILHEEGIP VTTVNKISDE KPHLLDVIRA
1010 1020 1030 1040 1050
GDAQFVINTL TRGKQPARDG FRIRRESVEN GVVCLTSLDT AEALLRVLES
1060
ITFSAESMPV MQ
Length:1,062
Mass (Da):117,814
Last modified:October 1, 2000 - v1
Checksum:i533DD62812EAA691
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB06255.1.
PIRiH83966.
RefSeqiWP_010898687.1. NC_002570.2.

Genome annotation databases

EnsemblBacteriaiBAB06255; BAB06255; BAB06255.
KEGGibha:BH2536.
PATRICi18942264. VBIBacHal18977_2645.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB06255.1.
PIRiH83966.
RefSeqiWP_010898687.1. NC_002570.2.

3D structure databases

ProteinModelPortaliQ9K9V9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272558.BH2536.

Proteomic databases

PRIDEiQ9K9V9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB06255; BAB06255; BAB06255.
KEGGibha:BH2536.
PATRICi18942264. VBIBacHal18977_2645.

Phylogenomic databases

eggNOGiENOG4105CU6. Bacteria.
COG0458. LUCA.
HOGENOMiHOG000234583.
KOiK01955.
OMAiSTAYMYS.
OrthoDBiPOG091H01IP.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00115.
BioCyciBHAL272558:GJC5-2620-MONOMER.

Family and domain databases

Gene3Di1.10.1030.10. 1 hit.
3.30.1490.20. 2 hits.
3.30.470.20. 2 hits.
3.40.50.1380. 1 hit.
3.40.50.20. 2 hits.
HAMAPiMF_01210_A. CPSase_L_chain_A. 1 hit.
MF_01210_B. CPSase_L_chain_B. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006275. CarbamoylP_synth_lsu.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR011607. MGS-like_dom.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PRINTSiPR00098. CPSASE.
SMARTiSM01096. CPSase_L_D3. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF48108. SSF48108. 1 hit.
SSF52335. SSF52335. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
PROSITEiPS50975. ATP_GRASP. 2 hits.
PS00866. CPSASE_1. 2 hits.
PS00867. CPSASE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCARB_BACHD
AccessioniPrimary (citable) accession number: Q9K9V9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.