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Protein

Carbamoyl-phosphate synthase pyrimidine-specific small chain

Gene

pyrAA

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase pyrimidine-specific small chain (pyrAA), Carbamoyl-phosphate synthase pyrimidine-specific large chain (pyrAB)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei246 – 2461NucleophileBy similarity
Active sitei331 – 3311By similarity
Active sitei333 – 3331By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBHAL272558:GJC5-2621-MONOMER.
UniPathwayiUPA00070; UER00115.

Protein family/group databases

MEROPSiC26.A33.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase pyrimidine-specific small chain (EC:6.3.5.5)
Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain
Gene namesi
Name:pyrAA
Ordered Locus Names:BH2537
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001258 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 362362Carbamoyl-phosphate synthase pyrimidine-specific small chainPRO_0000112251Add
BLAST

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.By similarity

Protein-protein interaction databases

STRINGi272558.BH2537.

Structurei

3D structure databases

ProteinModelPortaliQ9K9V8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini171 – 358188Glutamine amidotransferase type-1Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 167167CPSaseAdd
BLAST

Sequence similaritiesi

Belongs to the CarA family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105C1M. Bacteria.
COG0505. LUCA.
HOGENOMiHOG000038087.
KOiK01956.
OMAiCFSVQYH.
OrthoDBiPOG091H01NP.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain. 1 hit.
InterProiIPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
[Graphical view]
PfamiPF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SMARTiSM01097. CPSase_sm_chain. 1 hit.
[Graphical view]
SUPFAMiSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9K9V8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRQLILEDG SVFVGKGFGS DREMSGEVVF NTGMTGYQEM LSDPSYCGQI
60 70 80 90 100
VTLTYPLIGN YGINRDDFES MNPAIHGLIV KEACDIPSNW RSEESLDSLL
110 120 130 140 150
KAKQIPGLSG IDTRKLTRLI RMHGTLKGQL CPLDVDVEQI VQELKATPTP
160 170 180 190 200
TDQVSRVSTR DPYHVPGPGK RVVLVDYGMK HNILQELIRR NCEVFVVPYH
210 220 230 240 250
TSAEEVLRLG PDGVLLSNGP GNPEDVSEGV EMIRNLLGKV PLFGICLGHQ
260 270 280 290 300
LFALACGAKT EKLRFGHRGS NHPVRERSTG LIEITAQNHG YTVTEGSLVN
310 320 330 340 350
TDLIVTHEAV NDGTIEGLAH KVHPAFSVQY HPEASPGPED SNVLFDRFIK
360
LMESNKHRAL TV
Length:362
Mass (Da):39,872
Last modified:October 1, 2000 - v1
Checksum:iC7E537CA6403071C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB06256.1.
PIRiA83967.
RefSeqiWP_010898688.1. NC_002570.2.

Genome annotation databases

EnsemblBacteriaiBAB06256; BAB06256; BAB06256.
KEGGibha:BH2537.
PATRICi18942266. VBIBacHal18977_2646.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB06256.1.
PIRiA83967.
RefSeqiWP_010898688.1. NC_002570.2.

3D structure databases

ProteinModelPortaliQ9K9V8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272558.BH2537.

Protein family/group databases

MEROPSiC26.A33.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB06256; BAB06256; BAB06256.
KEGGibha:BH2537.
PATRICi18942266. VBIBacHal18977_2646.

Phylogenomic databases

eggNOGiENOG4105C1M. Bacteria.
COG0505. LUCA.
HOGENOMiHOG000038087.
KOiK01956.
OMAiCFSVQYH.
OrthoDBiPOG091H01NP.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00115.
BioCyciBHAL272558:GJC5-2621-MONOMER.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain. 1 hit.
InterProiIPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
[Graphical view]
PfamiPF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SMARTiSM01097. CPSase_sm_chain. 1 hit.
[Graphical view]
SUPFAMiSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCARA_BACHD
AccessioniPrimary (citable) accession number: Q9K9V8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.