Reviewed, UniProtKB/Swiss-Prot Q9K9V8 (CARA_BACHD)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase pyrimidine-specific small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	pyrAA
Ordered Locus Names:	BH2537

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity. HAMAP MF_01209
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	Carbamoyl-phosphate synthase pyrimidine-specific small chain HAMAP MF_01209		PRO_0000112251
Regions
	Domain	171 – 358	188	Glutamine amidotransferase type-1
	Region	1 – 167	167	CPSase HAMAP MF_01209
Sites
	Active site	246	1	Nucleophile By similarity
	Active site	331	1	By similarity
	Active site	333	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9K9V8-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: C7E537CA6403071C	FASTA	362	39,872
	10 20 30 40 50 60 MKRQLILEDG SVFVGKGFGS DREMSGEVVF NTGMTGYQEM LSDPSYCGQI VTLTYPLIGN 70 80 90 100 110 120 YGINRDDFES MNPAIHGLIV KEACDIPSNW RSEESLDSLL KAKQIPGLSG IDTRKLTRLI 130 140 150 160 170 180 RMHGTLKGQL CPLDVDVEQI VQELKATPTP TDQVSRVSTR DPYHVPGPGK RVVLVDYGMK 190 200 210 220 230 240 HNILQELIRR NCEVFVVPYH TSAEEVLRLG PDGVLLSNGP GNPEDVSEGV EMIRNLLGKV 250 260 270 280 290 300 PLFGICLGHQ LFALACGAKT EKLRFGHRGS NHPVRERSTG LIEITAQNHG YTVTEGSLVN 310 320 330 340 350 360 TDLIVTHEAV NDGTIEGLAH KVHPAFSVQY HPEASPGPED SNVLFDRFIK LMESNKHRAL TV

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB06256.1.
PIR	A83967.
RefSeq	NP_243403.1.
3D structure databases
SMR	Q9K9V8. Positions 1-356.
ModBase	Search...
Genome annotation databases
GeneID	891501.
GenomeReviews	Gene locus BH2537 in contig BA000004_GR.
KEGG	bha:BH2537.
NMPDR	fig|272558.1.peg.2537.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG286341.
OMA	FTYPELG.
PhylomeDB	Q9K9V8.
Enzyme and pathway databases
BioCyc	BHAL272558:BH2537-MONOMER.
BRENDA	6.3.5.5. 191865.
Family and domain databases
HAMAP	MF_01209_B. CPSase_S_chain_B. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase_dom. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_BACHD

Accession

Primary (citable) accession number: Q9K9V8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)