Q9K9V7 (PYRC_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroorotase
Short name=DHOase
EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	BH2538

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP MF_00220_B
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_00220_B
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. HAMAP MF_00220_B
Subunit structure	Homodimer By similarity. HAMAP MF_00220_B
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	Dihydroorotase HAMAP MF_00220_B		PRO_0000147228
Sites
	Metal binding	59	1	Zinc 1 By similarity
	Metal binding	61	1	Zinc 1 By similarity
	Metal binding	141	1	Zinc 1; via carbamate group By similarity
	Metal binding	141	1	Zinc 2; via carbamate group By similarity
	Metal binding	178	1	Zinc 2 By similarity
	Metal binding	231	1	Zinc 2 By similarity
	Metal binding	304	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	141	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9K9V7 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 657F6B920F62B32E	FASTA	428	46,284
	10 20 30 40 50 60 MKTLIKNGSI VTKEGTVKTQ DVLLHDGVIS AIGLNLEETD AHVIEANGKL VTPGLVDLHV 70 80 90 100 110 120 HLREPGGEAK ETIETGTKAA AKGGFTTVAA MPNTRPVPDS AEQMSWLTGR IDETGVVRVL 130 140 150 160 170 180 PYAAITTRQL GRELTDFAKL KEAGAFAFTD DGVGVQSAAM MLEAMKEAAK LEMAIVAHCE 190 200 210 220 230 240 ENTLIQNGSV HEGVFSKKHG IAGIPSVCES VHIARDVLLA EAAGVHYHVC HISTKESVRV 250 260 270 280 290 300 VRDAKRAGIR VTAEVTPHHL LLCDEDIPGL DAHYKMNPPL RGKEDREALI EGLLDGTIDF 310 320 330 340 350 360 IATDHAPHTE EEKGQGMERA PFGIVGLETA FPLLYTHFVK KGTFTLKQLV DWLTIDPAET 370 380 390 400 410 420 FQLPYGRLEE GAPADVTIID LEAERAIDPA HFASKGRNTP FAGWTCQGWP VATLVGGKLV WEEKEVAR

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB06257.1.
PIR	B83967.
RefSeq	NP_243404.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9K9V7.
ModBase	Search...
Protein family/group databases
MEROPS	M38.972.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000053628; EBBACP00000052267; EBBACG00000053619.
GeneID	891500.
GenomeReviews	Gene locus BH2538 in contig BA000004_GR.
KEGG	bha:BH2538.
NMPDR	fig|272558.1.peg.2538.
PATRIC	18942268. VBIBacHal18977_2647.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000001626.
HOGENOM	HBG724623.
OMA	CDVHPVG.
PhylomeDB	Q9K9V7.
ProtClustDB	PRK09357.
Enzyme and pathway databases
BioCyc	BHAL272558:BH2538-MONOMER.
Family and domain databases
HAMAP	MF_00220_B. PyrC_type2_B. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004722. DHOase. IPR002195. Dihydroorotase_CS. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
KO	K01465.
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
SUPFAM	SSF51338. Metalo_hydrolase. 1 hit.
TIGRFAMs	TIGR00857. PyrC_multi. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_BACHD

Accession

Primary (citable) accession number: Q9K9V7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program