Q9K9V2 (LSPA_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoprotein signal peptidase
EC=3.4.23.36

Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name=SPase II

Gene names

Name:	lspA
Synonyms:	lsp
Ordered Locus Names:	BH2543

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins. HAMAP MF_00161
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP MF_00161
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_00161.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 156	156	Lipoprotein signal peptidase HAMAP MF_00161		PRO_0000178768
Regions
	Topological domain	1	1	Cytoplasmic Potential
	Transmembrane	2 – 22	21	Helical; Potential
	Topological domain	23 – 51	29	Extracellular Potential
	Transmembrane	52 – 72	21	Helical; Potential
	Topological domain	73 – 84	12	Cytoplasmic Potential
	Transmembrane	85 – 105	21	Helical; Potential
	Topological domain	106 – 123	18	Extracellular Potential
	Transmembrane	124 – 144	21	Helical; Potential
	Topological domain	145 – 156	12	Cytoplasmic Potential
Sites
	Active site	102	1	By similarity
	Active site	129	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9K9V2 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 3670F52A55327B04	FASTA	156	17,828
	10 20 30 40 50 60 MIYYIVALVI ILLDQWTKWL VVRHMEIGES IPLLDSVLYL TSHRNKGAAF GILEGQMWLF 70 80 90 100 110 120 YIITSIVVIG IVYYMEKEAK HDRVFATALA LILGGAIGNF IDRIFRGEVV DFVNTYIFTY 130 140 150 NFPIFNVADS ALCVGVGILF LKMIRDERKA KKEKNA

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB06262.1.
PIR	G83967.
RefSeq	NP_243409.1. NC_002570.2.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	A08.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000051231; EBBACP00000049870; EBBACG00000051222.
GeneID	891512.
GenomeReviews	Gene locus BH2543 in contig BA000004_GR.
KEGG	bha:BH2543.
NMPDR	fig|272558.1.peg.2543.
PATRIC	18942278. VBIBacHal18977_2652.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000001848.
HOGENOM	HBG724422.
OMA	IASEIHG.
PhylomeDB	Q9K9V2.
ProtClustDB	PRK00376.
Enzyme and pathway databases
BioCyc	BHAL272558:BH2543-MONOMER.
Family and domain databases
HAMAP	MF_00161. LspA. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
KO	K03101.
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. LspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_BACHD

Accession

Primary (citable) accession number: Q9K9V2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program