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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei552Aminoacyl-adenylateUniRule annotation1
Binding sitei596ATPUniRule annotation1
Metal bindingi887ZincUniRule annotation1
Metal bindingi890ZincUniRule annotation1
Metal bindingi907ZincUniRule annotation1
Metal bindingi910ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBHAL272558:G1G3A-2617-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BH2545
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001258 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000983491 – 921Isoleucine--tRNA ligaseAdd BLAST921

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272558.BH2545

Structurei

3D structure databases

ProteinModelPortaliQ9K9V0
SMRiQ9K9V0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi593 – 597"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q9K9V0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFKETLLMP KTDFPMRGNL PNREPQMQEE WKDMNIYEKV QARTKGRPLF
60 70 80 90 100
VLHDGPPYAN GDIHMGHALN KVLKDMIVRY KSMAGFHAPY VPGWDTHGLP
110 120 130 140 150
IEQALTKSGV DRKSMSVAEF RKLCEEFARK QIDRQREQFM RLGVRGDWWN
160 170 180 190 200
PYVTLDKGFE AQQVKVFGEM AKKGYIYKGK KPVYWSPSSE SALAEAEIEY
210 220 230 240 250
HDKRSPSIYV AFPVKDGKNV LQQDEKIVIW TTTPWTIPAN LGIAVHPELE
260 270 280 290 300
YSVVAVKGEK YVVASGLVET LESALEWENP EILRTIKGVD LEYVVAEHPI
310 320 330 340 350
YGRDSLVVLG DHVTLDAGTG CVHTAPGHGE EDYIVGQKYG LDVLCPVDDK
360 370 380 390 400
GYMTAEAPGF EGLFYDEANK PITQKLDECG ALLKLTFITH SYAHDWRTKK
410 420 430 440 450
PVIYRATAQW FASIENFRDE LLRAIQEVKW VPEWGETRLY NMVRDRGDWC
460 470 480 490 500
ISRQRVWGVP IPIFYGENGE PIITDETINH VSELFRKHGS NVWFEWETKD
510 520 530 540 550
LLPEGFTHES SPNGQFTREM DIMDVWFDSG SSHQGVLVER EELDRPADLY
560 570 580 590 600
LEGSDQYRGW FNSSLSTSVA ITGKAPYKGV LSHGFTLDGE GKKMSKSLGN
610 620 630 640 650
VVIPNDVMKQ LGADILRLWV ASVDYQADVR VSDKILKQVS EVYRKIRNTY
660 670 680 690 700
RFLLGNLHDF HPATHRVAID QLNGLDRYML AKLNDVINRV KKAYDEYQFS
710 720 730 740 750
TVYHELHNFC TIELSSFYMD IAKDTLYVKH ADHPDRRATQ TVMYDVLVAL
760 770 780 790 800
AKLLSPIIPH TADEVWKHIP GVEEESVQLT DMPEPIELGD VSELKQKWDA
810 820 830 840 850
FINVRDDVLK ALENARNEKV IGKSLTAAIT LYADGDVRQL LEELGPLDKL
860 870 880 890 900
FIVSAVKVAG SVADAPKEAE SYDDLAIVVE KAVGETCERC WVVSPTVGTN
910 920
NEHPTLCADC AETVATYYVT K
Length:921
Mass (Da):104,563
Last modified:October 1, 2000 - v1
Checksum:i5D9A17ED5A741B9A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA Translation: BAB06264.1
PIRiA83968
RefSeqiWP_010898696.1, NC_002570.2

Genome annotation databases

EnsemblBacteriaiBAB06264; BAB06264; BAB06264
KEGGibha:BH2545

Similar proteinsi

Entry informationi

Entry nameiSYI_BACHD
AccessioniPrimary (citable) accession number: Q9K9V0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2000
Last modified: March 28, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health