Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9K9V0

- SYI_BACHD

UniProt

Q9K9V0 - SYI_BACHD

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521Aminoacyl-adenylateUniRule annotation
Binding sitei596 – 5961ATPUniRule annotation
Metal bindingi887 – 8871ZincUniRule annotation
Metal bindingi890 – 8901ZincUniRule annotation
Metal bindingi907 – 9071ZincUniRule annotation
Metal bindingi910 – 9101ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBHAL272558:GJC5-2629-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BH2545
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001258: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 921921Isoleucine--tRNA ligasePRO_0000098349Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272558.BH2545.

Structurei

3D structure databases

ProteinModelPortaliQ9K9V0.
SMRiQ9K9V0. Positions 2-882.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi593 – 5975"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9K9V0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDFKETLLMP KTDFPMRGNL PNREPQMQEE WKDMNIYEKV QARTKGRPLF
60 70 80 90 100
VLHDGPPYAN GDIHMGHALN KVLKDMIVRY KSMAGFHAPY VPGWDTHGLP
110 120 130 140 150
IEQALTKSGV DRKSMSVAEF RKLCEEFARK QIDRQREQFM RLGVRGDWWN
160 170 180 190 200
PYVTLDKGFE AQQVKVFGEM AKKGYIYKGK KPVYWSPSSE SALAEAEIEY
210 220 230 240 250
HDKRSPSIYV AFPVKDGKNV LQQDEKIVIW TTTPWTIPAN LGIAVHPELE
260 270 280 290 300
YSVVAVKGEK YVVASGLVET LESALEWENP EILRTIKGVD LEYVVAEHPI
310 320 330 340 350
YGRDSLVVLG DHVTLDAGTG CVHTAPGHGE EDYIVGQKYG LDVLCPVDDK
360 370 380 390 400
GYMTAEAPGF EGLFYDEANK PITQKLDECG ALLKLTFITH SYAHDWRTKK
410 420 430 440 450
PVIYRATAQW FASIENFRDE LLRAIQEVKW VPEWGETRLY NMVRDRGDWC
460 470 480 490 500
ISRQRVWGVP IPIFYGENGE PIITDETINH VSELFRKHGS NVWFEWETKD
510 520 530 540 550
LLPEGFTHES SPNGQFTREM DIMDVWFDSG SSHQGVLVER EELDRPADLY
560 570 580 590 600
LEGSDQYRGW FNSSLSTSVA ITGKAPYKGV LSHGFTLDGE GKKMSKSLGN
610 620 630 640 650
VVIPNDVMKQ LGADILRLWV ASVDYQADVR VSDKILKQVS EVYRKIRNTY
660 670 680 690 700
RFLLGNLHDF HPATHRVAID QLNGLDRYML AKLNDVINRV KKAYDEYQFS
710 720 730 740 750
TVYHELHNFC TIELSSFYMD IAKDTLYVKH ADHPDRRATQ TVMYDVLVAL
760 770 780 790 800
AKLLSPIIPH TADEVWKHIP GVEEESVQLT DMPEPIELGD VSELKQKWDA
810 820 830 840 850
FINVRDDVLK ALENARNEKV IGKSLTAAIT LYADGDVRQL LEELGPLDKL
860 870 880 890 900
FIVSAVKVAG SVADAPKEAE SYDDLAIVVE KAVGETCERC WVVSPTVGTN
910 920
NEHPTLCADC AETVATYYVT K
Length:921
Mass (Da):104,563
Last modified:October 1, 2000 - v1
Checksum:i5D9A17ED5A741B9A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB06264.1.
PIRiA83968.
RefSeqiNP_243411.1. NC_002570.2.
WP_010898696.1. NC_002570.2.

Genome annotation databases

EnsemblBacteriaiBAB06264; BAB06264; BAB06264.
GeneIDi891516.
KEGGibha:BH2545.
PATRICi18942282. VBIBacHal18977_2654.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB06264.1 .
PIRi A83968.
RefSeqi NP_243411.1. NC_002570.2.
WP_010898696.1. NC_002570.2.

3D structure databases

ProteinModelPortali Q9K9V0.
SMRi Q9K9V0. Positions 2-882.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272558.BH2545.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB06264 ; BAB06264 ; BAB06264 .
GeneIDi 891516.
KEGGi bha:BH2545.
PATRICi 18942282. VBIBacHal18977_2654.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BHAL272558:GJC5-2629-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis."
    Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.
    Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Entry informationi

Entry nameiSYI_BACHD
AccessioniPrimary (citable) accession number: Q9K9V0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2000
Last modified: November 26, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3