Q9K9T1 (MURB_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductase
EC=1.1.1.158

Alternative name(s):
UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	BH2564

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00037
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity. HAMAP MF_00037
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm By similarity HAMAP MF_00037.
Sequence similarities	Belongs to the MurB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000179176
Regions
	Domain	29 – 195	167	FAD-binding PCMH-type
Sites
	Active site	174	1	By similarity
	Active site	224	1	Proton donor By similarity
	Active site	294	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9K9T1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 7F315BA5631263BF	FASTA	301	32,899
	10 20 30 40 50 60 MEQLVESLRA LQVGEVRVNE SLAHHTTWKI GGPADVFVIP NDIEGLKNTM KLIQETGCKW 70 80 90 100 110 120 RVIGRGSNIL VSDKGLRGVT IKLDKGLDHL EVNGESITVG AGFPVVKLAT VISRQGLAGL 130 140 150 160 170 180 EFAAGIPGSV GGAVFMNAGA HGSDISQILT KAHVLFPDGT LRWLTNEEMA FSYRTSLLQK 190 200 210 220 230 240 NDGICVEAIF SLTRGDKEDI KKKLQKNKDY RRDTQPWNHP TCGSVFRNPL PEYAGQLIEK 250 260 270 280 290 300 AGLKGYQIGG AQISTMHANF IVNTGDAKAA DVLALIHHVK DTIQKQYQMN METEVELIGE R

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB06283.1.
PIR	D83970.
RefSeq	NP_243430.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9K9T1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000051053; EBBACP00000049692; EBBACG00000051044.
GeneID	894513.
GenomeReviews	Gene locus BH2564 in contig BA000004_GR.
KEGG	bha:BH2564.
NMPDR	fig|272558.1.peg.2564.
PATRIC	18942324. VBIBacHal18977_2675.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000001391.
HOGENOM	HBG686573.
OMA	EANVGRV.
PhylomeDB	Q9K9T1.
ProtClustDB	PRK13905.
Enzyme and pathway databases
BioCyc	BHAL272558:BH2564-MONOMER.
Family and domain databases
HAMAP	MF_00037. MurB. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
KO	K00075.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
SUPFAM	SSF56176. FAD-binding_2. 1 hit. SSF56194. MurB_C. 1 hit.
TIGRFAMs	TIGR00179. MurB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_BACHD

Accession

Primary (citable) accession number: Q9K9T1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program