Reviewed, UniProtKB/Swiss-Prot Q9K9S8 (MURD_BACHD)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	BH2567

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000108964
Regions
	Nucleotide binding	118 – 124	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9K9S8-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 76D54C6F549FB2ED	FASTA	450	48,757
	10 20 30 40 50 60 MKHTEQFHQK HILVLGLAKS GEAAARLLHD LGAIVTVNDQ KPLADNPQAQ KLQKEGIHVV 70 80 90 100 110 120 CGEHPISLLD GKELVVKNPG IRYDNPIVEE AIKRGISVVT EVELASKVSE AEIVAITGSN 130 140 150 160 170 180 GKTTTTSLVV EMLKGSAREP KVAGNIGVVA SDVAREATAD DVIVMEVSSF QLMGTSHFRP 190 200 210 220 230 240 KVAILLNIFD AHLDYHGSKE NYVAAKKKIV ENMKEEDYFV YNADDPLVSK VAAETKATPI 250 260 270 280 290 300 PFSRSTVVKS GAYVDGETYM FRGEKIVEKG DVVLPGDHNV DNVLAAMSAA LLMGATKEQI 310 320 330 340 350 360 HHVLSTFSGV EHRLQFVGTA FERKWYNDSK ATNILSTTAA IQSFTDPIVL LAGGLDRGNS 370 380 390 400 410 420 FDDLIPALQK VKAVVLFGET KHKLAQAAME AGVETIVEAE RVEDAVRKAL DVSANGDVIL 430 440 450 LSPACASWDQ YRTFEERGEA FVTSIEGLQE

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000004 Genomic DNA. Translation: BAB06286.1.
PIR	G83970.
RefSeq	NP_243433.1.
3D structure databases
HSSP	HSSP built from PDB template 2UAG based on UniProtKB P14900.
ModBase	Search...
Genome annotation databases
GeneID	892799.
GenomeReviews	Gene locus BH2567 in contig BA000004_GR.
KEGG	bha:BH2567.
NMPDR	fig|272558.1.peg.2567.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9K9S8.
OMA	SEDHLDR.
Enzyme and pathway databases
BioCyc	BHAL272558:BH2567-MON.
BRENDA	6.3.2.9. 191865.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BACHD

Accession

Primary (citable) accession number: Q9K9S8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)