Reviewed, UniProtKB/Swiss-Prot Q9K9S6 (MRAY_BACHD)

Names and origin

Protein names

Recommended name:
    Phospho-N-acetylmuramoyl-pentapeptide-transferase
    EC=2.7.8.13
Alternative name(s):
    UDP-MurNAc-pentapeptide phosphotransferase

Gene names

Name:	mraY
Ordered Locus Names:	BH2568/BH2569

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity.
Catalytic activity	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 4 family. MraY subfamily.
Sequence caution	The sequence BAB06288.1 differs from that shown. Reason: Frameshift at position 78. Produces two separate ORFs.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038		PRO_0000108779
Regions
	Transmembrane	5 – 25	21	Potential
	Transmembrane	57 – 77	21	Potential
	Transmembrane	81 – 101	21	Potential
	Transmembrane	117 – 137	21	Potential
	Transmembrane	146 – 166	21	Potential
	Transmembrane	178 – 198	21	Potential
	Transmembrane	200 – 220	21	Potential
	Transmembrane	227 – 247	21	Potential
	Transmembrane	252 – 272	21	Potential
	Transmembrane	304 – 324	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9K9S6-1 [UniParc]. Last modified April 23, 2003. Version 2. Checksum: C23434161DA41B8A	FASTA	325	35,377
	10 20 30 40 50 60 MLERVLLLTL ILSFVITVIL SPIFIPFLRR LKFGQSIREE GPKSHQKKSG TPTMGGLMIL 70 80 90 100 110 120 LSILVSSLFV SFQLSIFSMD VLLLLLVTIG FGVLGFIDDF IKVVMKRNLG LTSKQKLIGQ 130 140 150 160 170 180 LVVAVLFYLG LRNMGLSTEV TIPATSLSID FGWFYLPLVI VMLVGASNAV NLTDGLDGLV 190 200 210 220 230 240 AGTGAIAFGA FAIIAWATNY FEVAIFSAAV VGAVLGFLVF NSHPAKVFMG DTGSLALGGA 250 260 270 280 290 300 IAAIAIMAKQ EILLIIIGGV FVIETLSVII QVISFKTRGK RIFKMSPLHH HYELSGWSEW 310 320 RVVVTFWTVG LLFAMLAIYL EVWIT

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000004 Genomic DNA. Translation: BAB06287.1. Frameshift. BA000004 Genomic DNA. Translation: BAB06288.1. Frameshift.
PIR	A83971. H83970.
RefSeq	NP_243434.1. NP_243435.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	892680. 892870.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9K9S6.
Enzyme and pathway databases
BioCyc	BHAL272558:BH2568-MON. BHAL272558:BH2569-MON.
BRENDA	2.7.8.13. 191865.
Family and domain databases
HAMAP	MF_00038. [Tree]
InterPro	IPR000715. Glycosyl_transferase_4. IPR018481. Glycosyl_Trfase_4_cons-reg. IPR003524. PNAcMuramoyl-5peptid_Trfase. IPR018480. PNAcMuramoyl-5peptid_Trfase_CS. [Graphical view]
PANTHER	PTHR22926. Glyco_trans_4. 1 hit. PTHR22926:SF3. PNAcPpept_trans. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. PF10555. MraY_sig1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00445. mraY. 1 hit.
PROSITE	PS01347. MRAY_1. 1 hit. PS01348. MRAY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MRAY_BACHD

Accession

Primary (citable) accession number: Q9K9S6
Secondary accession number(s): Q9K9S7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	April 23, 2003
Last modified:	October 13, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)