Q9K9I9 (DEF_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptide deformylase
Short name=PDF
EC=3.5.1.88

Alternative name(s):
Polypeptide deformylase

Gene names

Name:	def
Ordered Locus Names:	BH2658

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	182 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity. HAMAP MF_00163
Catalytic activity	Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163
Cofactor	Binds 1 Fe2+ ion By similarity. HAMAP MF_00163
Sequence similarities	Belongs to the polypeptide deformylase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	Iron Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: InterPro peptide deformylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 182	182	Peptide deformylase HAMAP MF_00163		PRO_0000082737
Sites
	Active site	154	1	By similarity
	Metal binding	110	1	Iron By similarity
	Metal binding	153	1	Iron By similarity
	Metal binding	157	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9K9I9-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 665D39B56EE6153F	FASTA	182	20,599
	10 20 30 40 50 60 MLTMKDIVRE GNPVLREVAK PVPVPLSDED KQTAKRMLEF LINSQNPEIA EKYSLRPGVG 70 80 90 100 110 120 LAAPQIGLSK QMIAVHTTDE NEKEYSLVLF NPKIISESVE MTHLEGGEGC LSVDREVQGI 130 140 150 160 170 180 VPRHARITVK AINENNEEVR LKLKGFPAIV FQHEIDHLNG IMFYDRIEGW VDPYKREINP SL

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB06377.1.
PIR	B83982.
RefSeq	NP_243524.1.
3D structure databases
ProteinModelPortal	Q9K9I9.
SMR	Q9K9I9. Positions 1-167.
ModBase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000051031; EBBACP00000049670; EBBACG00000051022.
GeneID	893733.
GenomeReviews	Gene locus BH2658 in contig BA000004_GR.
KEGG	bha:BH2658.
NMPDR	fig|272558.1.peg.2658.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG665227.
OMA	DIIKEGH.
ProtClustDB	PRK00150.
Enzyme and pathway databases
BioCyc	BHAL272558:BH2658-MONOMER.
BRENDA	3.5.1.88. 191865.
Family and domain databases
HAMAP	MF_00163. Pep_deformylase. [Tree]
InterPro	IPR000181. Fmet_deformylase. [Graphical view]
Gene3D	G3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
PANTHER	PTHR10458. Fmet_deformylase. 1 hit.
Pfam	PF01327. Pep_deformylase. 1 hit. [Graphical view]
PIRSF	PIRSF004749. Pep_def. 1 hit.
PRINTS	PR01576. PDEFORMYLASE.
SUPFAM	SSF56420. Fmet_deformylase. 1 hit.
TIGRFAMs	TIGR00079. pept_deformyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DEF_BACHD

Accession

Primary (citable) accession number: Q9K9I9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 5, 2001
Last sequence update:	October 1, 2000
Last modified:	August 10, 2010
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)