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Protein

Isocitrate lyase

Gene

aceA

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the metabolic adaptation in response to environmental changes. Catalyzes the reversible formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle during growth on fatty acid substrates.By similarity

Catalytic activityi

Isocitrate = succinate + glyoxylate.By similarity

Cofactori

Mg2+By similarity

Pathwayi: glyoxylate cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from isocitrate.By similarity
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Isocitrate lyase (aceA)
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi150MagnesiumBy similarity1
Active sitei188Proton acceptorBy similarity1
Binding sitei225SubstrateBy similarity1
Binding sitei344SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciBHAL272558:G1G3A-2747-MONOMER
UniPathwayiUPA00703; UER00719

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyaseBy similarity (EC:4.1.3.1By similarity)
Short name:
ICLBy similarity
Alternative name(s):
IsocitraseBy similarity
IsocitrataseBy similarity
Gene namesi
Name:aceA
Ordered Locus Names:BH2677
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001258 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000687711 – 427Isocitrate lyaseAdd BLAST427

Proteomic databases

PRIDEiQ9K9H0

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi272558.BH2677

Structurei

3D structure databases

ProteinModelPortaliQ9K9H0
SMRiQ9K9H0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni89 – 91Substrate bindingBy similarity3
Regioni189 – 190Substrate bindingBy similarity2
Regioni310 – 314Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2224 LUCA
HOGENOMiHOG000238475
KOiK01637
OMAiLEKDWAE
OrthoDBiPOG091H04OH

Family and domain databases

InterProiView protein in InterPro
IPR006254 Isocitrate_lyase
IPR018523 Isocitrate_lyase_ph_CS
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
PANTHERiPTHR21631 PTHR21631, 1 hit
PfamiView protein in Pfam
PF00463 ICL, 1 hit
PIRSFiPIRSF001362 Isocit_lyase, 1 hit
SUPFAMiSSF51621 SSF51621, 1 hit
TIGRFAMsiTIGR01346 isocit_lyase, 1 hit
PROSITEiView protein in PROSITE
PS00161 ISOCITRATE_LYASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q9K9H0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGWQETAEK LEMSWSNDVR WQGVERPYSG EEVVKLRGSL KIEYTLAKTG
60 70 80 90 100
AEKLWKLLHE EDYVNALGAM TGGQAIQQVK AGLKAIYLSG WQVAADANLA
110 120 130 140 150
GHMYPDQSLY PANSVPSVVK RINQALQRAD QIQHLEGEGE VDYFAPIVAD
160 170 180 190 200
AEAGFGGQLN VFELMKAMIE AGASGVHFED QLASEKKCGH LGGKVLIPTQ
210 220 230 240 250
TAIRNLVSAR LAADVMGVPT ILVARTDADA ADLITSDIDP ADQRFITGER
260 270 280 290 300
TPEGFYRTNA GIEQAIARGL AYAPYADLIW CETSKPSLEE AKQFADAIHE
310 320 330 340 350
KFPGKLLAYN CSPSFNWEAN LDRATIETFQ QELGKMGYKF QFVTLAGFHA
360 370 380 390 400
LNHSMFELAY GYKQRGMGAY SELQQAEFAS EVKGYTATRH QREVGTGYFD
410 420
QVAQTITGGT SSTTALTGST EEAQFQK
Length:427
Mass (Da):46,783
Last modified:October 1, 2000 - v1
Checksum:i0C52D4A9F89E0DFE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA Translation: BAB06396.1
PIRiE83984
RefSeqiWP_010898826.1, NC_002570.2

Genome annotation databases

EnsemblBacteriaiBAB06396; BAB06396; BAB06396
KEGGibha:BH2677

Similar proteinsi

Entry informationi

Entry nameiACEA_BACHD
AccessioniPrimary (citable) accession number: Q9K9H0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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