Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isocitrate lyase

Gene

aceA

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the metabolic adaptation in response to environmental changes. Catalyzes the reversible formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle during growth on fatty acid substrates.By similarity

Catalytic activityi

Isocitrate = succinate + glyoxylate.By similarity

Cofactori

Mg2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi150 – 1501MagnesiumBy similarity
Active sitei188 – 1881Proton acceptorBy similarity
Binding sitei225 – 2251SubstrateBy similarity
Binding sitei344 – 3441SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBHAL272558:GJC5-2761-MONOMER.
RETL1328306-WGS:GSTH-727-MONOMER.
UniPathwayiUPA00703; UER00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyaseBy similarity (EC:4.1.3.1By similarity)
Short name:
ICLBy similarity
Alternative name(s):
IsocitraseBy similarity
IsocitrataseBy similarity
Gene namesi
Name:aceA
Ordered Locus Names:BH2677
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001258 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 427427Isocitrate lyasePRO_0000068771Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi272558.BH2677.

Structurei

3D structure databases

ProteinModelPortaliQ9K9H0.
SMRiQ9K9H0. Positions 3-425.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni89 – 913Substrate bindingBy similarity
Regioni189 – 1902Substrate bindingBy similarity
Regioni310 – 3145Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2224.
HOGENOMiHOG000238475.
KOiK01637.
OMAiRWKGVTR.
OrthoDBiEOG689HMX.

Family and domain databases

Gene3Di3.20.20.60. 1 hit.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9K9H0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGWQETAEK LEMSWSNDVR WQGVERPYSG EEVVKLRGSL KIEYTLAKTG
60 70 80 90 100
AEKLWKLLHE EDYVNALGAM TGGQAIQQVK AGLKAIYLSG WQVAADANLA
110 120 130 140 150
GHMYPDQSLY PANSVPSVVK RINQALQRAD QIQHLEGEGE VDYFAPIVAD
160 170 180 190 200
AEAGFGGQLN VFELMKAMIE AGASGVHFED QLASEKKCGH LGGKVLIPTQ
210 220 230 240 250
TAIRNLVSAR LAADVMGVPT ILVARTDADA ADLITSDIDP ADQRFITGER
260 270 280 290 300
TPEGFYRTNA GIEQAIARGL AYAPYADLIW CETSKPSLEE AKQFADAIHE
310 320 330 340 350
KFPGKLLAYN CSPSFNWEAN LDRATIETFQ QELGKMGYKF QFVTLAGFHA
360 370 380 390 400
LNHSMFELAY GYKQRGMGAY SELQQAEFAS EVKGYTATRH QREVGTGYFD
410 420
QVAQTITGGT SSTTALTGST EEAQFQK
Length:427
Mass (Da):46,783
Last modified:October 1, 2000 - v1
Checksum:i0C52D4A9F89E0DFE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB06396.1.
PIRiE83984.
RefSeqiNP_243543.1. NC_002570.2.
WP_010898826.1. NC_002570.2.

Genome annotation databases

EnsemblBacteriaiBAB06396; BAB06396; BAB06396.
KEGGibha:BH2677.
PATRICi18942542. VBIBacHal18977_2784.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB06396.1.
PIRiE83984.
RefSeqiNP_243543.1. NC_002570.2.
WP_010898826.1. NC_002570.2.

3D structure databases

ProteinModelPortaliQ9K9H0.
SMRiQ9K9H0. Positions 3-425.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272558.BH2677.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB06396; BAB06396; BAB06396.
KEGGibha:BH2677.
PATRICi18942542. VBIBacHal18977_2784.

Phylogenomic databases

eggNOGiCOG2224.
HOGENOMiHOG000238475.
KOiK01637.
OMAiRWKGVTR.
OrthoDBiEOG689HMX.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.
BioCyciBHAL272558:GJC5-2761-MONOMER.
RETL1328306-WGS:GSTH-727-MONOMER.

Family and domain databases

Gene3Di3.20.20.60. 1 hit.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis."
    Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.
    Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Entry informationi

Entry nameiACEA_BACHD
AccessioniPrimary (citable) accession number: Q9K9H0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 1, 2000
Last modified: April 29, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.