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Protein

Beta-galactosidase

Gene

lacZ

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei460 – 4601Proton donorBy similarity
Active sitei527 – 5271NucleophileBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciBHAL272558:GJC5-2807-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
Ordered Locus Names:BH2723
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001258 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10141014Beta-galactosidasePRO_0000057658Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272558.BH2723.

Structurei

3D structure databases

ProteinModelPortaliQ9K9C6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252444.
KOiK01190.
OMAiTNRHEHH.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9K9C6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTHSNVSWL RDVNVFAVNR LPAHSDHVYY ETVEEAKKEP PMSMRHSLNG
60 70 80 90 100
HWKFHYAINP NTRPKEFYQL GFDCKCWDDI LVPGHIQLQG YGKPQYVNTM
110 120 130 140 150
YPWDGHHHLR PPEIPEDDNP VGSYVKYFDI PNNMSNHPLF ISFQGVETAF
160 170 180 190 200
YVWLNGEFVG YSEDSFTPAE FDLTPYAVEG ENKLCVEVYQ RSTGSWLEDQ
210 220 230 240 250
DFWRFSGIFR DVYLYTIPNI HVYDMHVRAD LDRSLQTGIL ETTLELKRSQ
260 270 280 290 300
EKEVMIVAEL YDAEGAVVAT ADMKTNQDQA TVSMSVDSPA LWSAEDPYLY
310 320 330 340 350
KLFLKLFDEN GTLVEVVPQK IGFRRFELVN NIMTLNGKRI VFKGVNRHEF
360 370 380 390 400
NGRTGRVVTK EDMLEDIKTM KKHNINAVRT SHYPNNSEWY QLCDEYGLYV
410 420 430 440 450
IDEMNLETHG SWQKLGKVEP SWNIPGNHLE WEPIVMDRAV SMFERDKNHP
460 470 480 490 500
SILIWSCGNE SYAGEVILNV SRYFKSVDPS RLVHYEGVFH ARAYDATSDM
510 520 530 540 550
ESRMYAKPKD IEDYLTNDPK KPYISCEYMH AMGNSLGGMH KYTELEQKYP
560 570 580 590 600
MYQGGFIWDY IDQALLKKDR YGKEYFAYGG DFGDRPTDYS FCANGIVYAD
610 620 630 640 650
RKPSPKMQEV KFLYQNIKLV PDREGVLVKN ENLFTDTSAY QLEYVLYWEG
660 670 680 690 700
TELYRKKQDV FVAPQEEVYL PFDWLEQGMN ESGEYCIHTM LTLKQDQLWA
710 720 730 740 750
EKGHEVAFGQ HVYRMGAIQK ERNARGALKV VHGDVNIGIH GEDFSVLFSK
760 770 780 790 800
AVGSLVSLHY AGKEMIEQPP MPLFWRATTD NDKGCSQLYH SGIWYAASLA
810 820 830 840 850
RKCVNMEVEE KPGHVSVLFT YHFAISDRVE VKVGYTVFPD GSLRVRSTYQ
860 870 880 890 900
ASKGGETLPQ LPMFALSFKL PADYEQLEWY ALGPEENYAD RATGARLCTF
910 920 930 940 950
KNKVEDSLSQ YVTPQESGNR TGVRTVKILD ANGQGIEVCS VEEPIECQIS
960 970 980 990 1000
PYTAFELEQA SHPYELPNVH YTVVNVAGKQ MGVGGDDSWG APVHDEYVLK
1010
ADQDLEFVFD INRV
Length:1,014
Mass (Da):116,656
Last modified:October 1, 2000 - v1
Checksum:i30405EB697C72798
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB06442.1.
PIRiC83990.
RefSeqiNP_243589.1. NC_002570.2.
WP_010898871.1. NC_002570.2.

Genome annotation databases

EnsemblBacteriaiBAB06442; BAB06442; BAB06442.
KEGGibha:BH2723.
PATRICi18942632. VBIBacHal18977_2829.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB06442.1.
PIRiC83990.
RefSeqiNP_243589.1. NC_002570.2.
WP_010898871.1. NC_002570.2.

3D structure databases

ProteinModelPortaliQ9K9C6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272558.BH2723.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB06442; BAB06442; BAB06442.
KEGGibha:BH2723.
PATRICi18942632. VBIBacHal18977_2829.

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252444.
KOiK01190.
OMAiTNRHEHH.
OrthoDBiEOG6XWV0T.

Enzyme and pathway databases

BioCyciBHAL272558:GJC5-2807-MONOMER.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis."
    Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.
    Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Entry informationi

Entry nameiBGAL_BACHD
AccessioniPrimary (citable) accession number: Q9K9C6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: October 1, 2000
Last modified: April 1, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.