Q9K9C6 (BGAL_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactosidase
Short name=Beta-gal
EC=3.2.1.23

Alternative name(s):
Lactase

Gene names

Name:	lacZ
Ordered Locus Names:	BH2723

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus halodurans

Protein attributes

Sequence length	1014 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	beta-galactosidase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	beta-galactosidase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1014	1014	Beta-galactosidase		PRO_0000057658
Sites
	Active site	460	1	Proton donor By similarity
	Active site	527	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9K9C6 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 30405EB697C72798	FASTA	1,014	116,656
	10 20 30 40 50 60 MKTHSNVSWL RDVNVFAVNR LPAHSDHVYY ETVEEAKKEP PMSMRHSLNG HWKFHYAINP 70 80 90 100 110 120 NTRPKEFYQL GFDCKCWDDI LVPGHIQLQG YGKPQYVNTM YPWDGHHHLR PPEIPEDDNP 130 140 150 160 170 180 VGSYVKYFDI PNNMSNHPLF ISFQGVETAF YVWLNGEFVG YSEDSFTPAE FDLTPYAVEG 190 200 210 220 230 240 ENKLCVEVYQ RSTGSWLEDQ DFWRFSGIFR DVYLYTIPNI HVYDMHVRAD LDRSLQTGIL 250 260 270 280 290 300 ETTLELKRSQ EKEVMIVAEL YDAEGAVVAT ADMKTNQDQA TVSMSVDSPA LWSAEDPYLY 310 320 330 340 350 360 KLFLKLFDEN GTLVEVVPQK IGFRRFELVN NIMTLNGKRI VFKGVNRHEF NGRTGRVVTK 370 380 390 400 410 420 EDMLEDIKTM KKHNINAVRT SHYPNNSEWY QLCDEYGLYV IDEMNLETHG SWQKLGKVEP 430 440 450 460 470 480 SWNIPGNHLE WEPIVMDRAV SMFERDKNHP SILIWSCGNE SYAGEVILNV SRYFKSVDPS 490 500 510 520 530 540 RLVHYEGVFH ARAYDATSDM ESRMYAKPKD IEDYLTNDPK KPYISCEYMH AMGNSLGGMH 550 560 570 580 590 600 KYTELEQKYP MYQGGFIWDY IDQALLKKDR YGKEYFAYGG DFGDRPTDYS FCANGIVYAD 610 620 630 640 650 660 RKPSPKMQEV KFLYQNIKLV PDREGVLVKN ENLFTDTSAY QLEYVLYWEG TELYRKKQDV 670 680 690 700 710 720 FVAPQEEVYL PFDWLEQGMN ESGEYCIHTM LTLKQDQLWA EKGHEVAFGQ HVYRMGAIQK 730 740 750 760 770 780 ERNARGALKV VHGDVNIGIH GEDFSVLFSK AVGSLVSLHY AGKEMIEQPP MPLFWRATTD 790 800 810 820 830 840 NDKGCSQLYH SGIWYAASLA RKCVNMEVEE KPGHVSVLFT YHFAISDRVE VKVGYTVFPD 850 860 870 880 890 900 GSLRVRSTYQ ASKGGETLPQ LPMFALSFKL PADYEQLEWY ALGPEENYAD RATGARLCTF 910 920 930 940 950 960 KNKVEDSLSQ YVTPQESGNR TGVRTVKILD ANGQGIEVCS VEEPIECQIS PYTAFELEQA 970 980 990 1000 1010 SHPYELPNVH YTVVNVAGKQ MGVGGDDSWG APVHDEYVLK ADQDLEFVFD INRV

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB06442.1.
PIR	C83990.
RefSeq	NP_243589.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9K9C6.
ModBase	Search...
Protein-protein interaction databases
STRING	272558.BH2723.
Protein family/group databases
CAZy	GH2. Glycoside Hydrolase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB06442; BAB06442; BAB06442.
GeneID	893545.
KEGG	bha:BH2723.
PATRIC	18942632. VBIBacHal18977_2829.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3250.
HOGENOM	HOG000252444.
KO	K01190.
OMA	QNEATEW.
ProtClustDB	CLSK793204.
Enzyme and pathway databases
BioCyc	BHAL272558:GJC5-2807-MONOMER.
Family and domain databases
Gene3D	2.60.40.320. 2 hits. 2.70.98.10. 1 hit. 3.20.20.80. 1 hit.
InterPro	IPR004199. B-gal_small/dom_5. IPR011013. Gal_mutarotase_SF_dom. IPR008979. Galactose-bd-like. IPR014718. Glyco_hydro-type_carb-bd_sub. IPR006101. Glyco_hydro_2. IPR013812. Glyco_hydro_2/20_Ig-like. IPR023232. Glyco_hydro_2_AS. IPR023230. Glyco_hydro_2_CS. IPR006102. Glyco_hydro_2_Ig-like. IPR006104. Glyco_hydro_2_N. IPR006103. Glyco_hydro_2_TIM. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF02929. Bgal_small_N. 1 hit. PF00703. Glyco_hydro_2. 1 hit. PF02836. Glyco_hydro_2_C. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view]
PRINTS	PR00132. GLHYDRLASE2.
SMART	SM01038. Bgal_small_N. 1 hit. [Graphical view]
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF74650. Gal_mut_like. 1 hit. SSF49303. Glyco_hydro_2Ig. 2 hits. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit. PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_BACHD

Accession

Primary (citable) accession number: Q9K9C6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2002
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program