Reviewed, UniProtKB/Swiss-Prot Q9K9B2 (ROCA1_BACHD)

Names and origin

Protein names

Recommended name:
    1-pyrroline-5-carboxylate dehydrogenase 1
      Short name=P5C dehydrogenase 1
    EC=1.5.1.12

Gene names

Name:	rocA1
Ordered Locus Names:	BH2737

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H. HAMAP MF_00733
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2. HAMAP MF_00733
Sequence similarities	Belongs to the aldehyde dehydrogenase family. RocA subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	1-pyrroline-5-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 515	515	1-pyrroline-5-carboxylate dehydrogenase 1 HAMAP MF_00733		PRO_0000056507
Sites
	Active site	286	1	By similarity
	Active site	320	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9K9B2-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 1AA5F76CA3093749	FASTA	515	56,642
	10 20 30 40 50 60 MLQPYKHEPF TDFTVEANRK AFEEALGLVE KELGKEYPLI INGERVTTED KIQSWNPARK 70 80 90 100 110 120 DQLVGSVSKA NQDLAEKAIQ SADEAFQTWR NVNPEERANI LVKAAAIIRR RKHEFSAWLV 130 140 150 160 170 180 HEAGKPWKEA DADTAEAIDF LEYYARQMIE LNRGKEILSR PGEQNRYFYT PMGVTVTISP 190 200 210 220 230 240 WNFALAIMVG TAVAPIVTGN TVVLKPASTT PVVAAKFVEV LEDAGLPKGV INYVPGSGAE 250 260 270 280 290 300 VGDYLVDHPK TSLITFTGSK DVGVRLYERA AVVRPGQNHL KRVIVEMGGK DTVVVDRDAD 310 320 330 340 350 360 LDLAAESILV SAFGFSGQKC SAGSRAVIHK DVYDEVLEKT VALAKNLTVG DPTNRDNYMG 370 380 390 400 410 420 PVIDEKAFEK IMSYIEIGKK EGRLMTGGEG DSSTGFFIQP TIIADLDPEA VIMQEEIFGP 430 440 450 460 470 480 VVAFSKANDF DHALEIANNT EYGLTGAVIT RNRAHIEQAK REFHVGNLYF NRNCTGAIVG 490 500 510 YHPFGGFKMS GTDSKAGGPD YLALHMQAKT VSEMY

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000004 Genomic DNA. Translation: BAB06456.1.
PIR	A83992.
RefSeq	NP_243603.1.
3D structure databases
HSSP	HSSP built from PDB template 1BXS based on UniProtKB P51977.
ModBase	Search...
Genome annotation databases
GeneID	893516.
GenomeReviews	Gene locus BH2737 in contig BA000004_GR.
KEGG	bha:BH2737.
NMPDR	fig|272558.1.peg.2737.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9K9B2.
OMA	Q9K9B2. EVSKSWA.
Enzyme and pathway databases
BioCyc	BHAL272558:BH2737-MON.
BRENDA	1.5.1.12. 191865.
Family and domain databases
HAMAP	MF_00733. [Tree]
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR005932. d-1-pyrroline-5-COlate_DH-2. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01237. D1pyr5carbox2. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ROCA1_BACHD

Accession

Primary (citable) accession number: Q9K9B2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)