Reviewed, UniProtKB/Swiss-Prot Q9K997 (DCTS_BACHD)

Names and origin

Protein names

Recommended name:
    Probable C4-dicarboxylate sensor kinase
    EC=2.7.13.3

Gene names

Name:	dctS
Ordered Locus Names:	BH2752

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Member of the two-component regulatory system dctS/dctR. Probably activates dctR by phosphorylation. Essential for expression of dctP By similarity.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Contains 1 histidine kinase domain. Contains 1 PAS (PER-ARNT-SIM) domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 532	532	Probable C4-dicarboxylate sensor kinase		PRO_0000074727
Regions
	Topological domain	1 – 12	12	Cytoplasmic Potential
	Transmembrane	13 – 33	21	Potential
	Topological domain	34 – 175	142	Extracellular Potential
	Transmembrane	176 – 196	21	Potential
	Topological domain	197 – 532	336	Cytoplasmic Potential
	Domain	216 – 279	64	PAS
	Domain	315 – 531	217	Histidine kinase
Amino acid modifications
	Modified residue	339	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9K997-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 5C2445CEA0119A12	FASTA	532	59,999
	10 20 30 40 50 60 MRLFRQLSIQ WKITILSFGI VAFALMMVSI SLLGYVTSIK EDELSNRTMI TAQLVAQNHT 70 80 90 100 110 120 VQQWVDAKPE EASRTLQPIV ERIRVINDHD YIVLLNMDRI RITHPIPERL QTPFVGGDED 130 140 150 160 170 180 PAFAEHIYLS KAKTEGVVTV RAFMPILNQQ REQVGVAVVG SVLPSYADMI QEFWQPALLI 190 200 210 220 230 240 GLITALFGFW GSWLLASHIK RQTFNMEPDE LAHLLVERDA SFNAIHEGVV AINKHEKITI 250 260 270 280 290 300 MNEAARRMLG VKEKAIGRNI HEVIPDTKLP EILSIGKPLY QREFYIQGRL VFSNRIPIQI 310 320 330 340 350 360 DGETVGAIAI FQDKSDVDRL AEELTGVQAF VDALRVQNHE YSNKLHTIAG LIQLDEGKKA 370 380 390 400 410 420 LQYIFDLEEE QEEFSGVVMQ KIHNDSLAGL LLGKVSRGKE LGVQVIIEKD SEFIDHPEGV 430 440 450 460 470 480 TTHDLVVIVG NLIDNSLDAF SSTQDQNKTV HVFIGEENDF LKIRVRDNGE GIREEVREKM 490 500 510 520 530 FVRGFSTKST SGRGIGLFLI QAIVERVEGK IEVESELNIG TTFSIYLPKK RG

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB06471.1.
PIR	H83993.
RefSeq	NP_243618.1.
3D structure databases
SMR	Q9K997. Positions 37-172, 217-319, 327-514, 424-529.
ModBase	Search...
Genome annotation databases
GeneID	893487.
GenomeReviews	Gene locus BH2752 in contig BA000004_GR.
KEGG	bha:BH2752.
NMPDR	fig|272558.1.peg.2752.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG537380.
OMA	SNRIPIQ.
PhylomeDB	Q9K997.
Enzyme and pathway databases
BioCyc	BHAL272558:BH2752-MONOMER.
BRENDA	2.7.13.3. 191865.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR000014. PAS. IPR013767. PAS_fold. IPR004358. Sig_transdc_His_kin-like_C. IPR016120. Sig_transdc_His_kin_SpoOB. IPR005467. Sig_transdc_His_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF02518. HATPase_c. 1 hit. PF00989. PAS. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00387. HATPase_c. 1 hit. SM00091. PAS. 1 hit. [Graphical view]
PROSITE	PS50109. HIS_KIN. 1 hit. PS50112. PAS. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCTS_BACHD

Accession

Primary (citable) accession number: Q9K997

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 14, 2001
Last sequence update:	October 1, 2000
Last modified:	January 19, 2010
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)