Q9K971 (DXS_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	BH2779

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	629 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 629	629	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_0000189085

Sequences

	Sequence		Length	Mass (Da)
	Q9K971 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 7B5909B2F427C11E	FASTA	629	69,279
	10 20 30 40 50 60 MDLEKLHDPS LIKSMTKQEL EQLAEEIRQF LIEKLSITGG HLGPNLGVVE LTLALHSLFD 70 80 90 100 110 120 SPKDKLIWDV GHQAYVHKIL TGRAGQFDQL RQYKGLCGFP KRDESEHDVW ETGHSSTSLS 130 140 150 160 170 180 AAMGMATARD LKGTDENVIA IIGDGALTGG MALEALNHIG HEQKDLIVVL NDNEMSIAPN 190 200 210 220 230 240 VGALHNVLGR LRTAGKYQKA KEDLEMLIKK IPAFGGKLAQ AAERVKDSLK YLLVSGIFFE 250 260 270 280 290 300 EMGFTYLGPV DGHDLDDLME NLKYAKKTKG PVLIHVLTKK GKGYAPAEND EKGTWHGVGH 310 320 330 340 350 360 YKIESGELVK KPAPPSYSGV FAETLKKIAR NDPRIVALTA AMPGGTKLDQ FAKEFPDRMF 370 380 390 400 410 420 DVGIAEQHAT TMAGGLATQG LKPVFAVYST FLQRGYDQVV HDICRQNLNV FFAIDRAGLV 430 440 450 460 470 480 GADGETHQGV FDIAYLRHLP NMKILMPKDE NELQHMVYTA IQYEGGPIAV RYPRGNGYGI 490 500 510 520 530 540 KMDEVLKEIP IGSWEVLQEG TDACILTFGT MIPVAEQASK ELSQQGYSIR LINARSVKPL 550 560 570 580 590 600 DEAMLHEIAK SGRPVLTLEE TAVQGSFGSA VLEFFHDHGY HNVVTQRMGI PDRFIEHGSV 610 620 SELLEEIGLT SSQVANQLSK LLPRKQKRA

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB06498.1.
PIR	C83997.
RefSeq	NP_243645.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9K971.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000052642; EBBACP00000051281; EBBACG00000052633.
GeneID	893421.
GenomeReviews	Gene locus BH2779 in contig BA000004_GR.
KEGG	bha:BH2779.
NMPDR	fig|272558.1.peg.2779.
PATRIC	18942746. VBIBacHal18977_2884.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00070000031844.
HOGENOM	HBG571647.
OMA	EAMNAHA.
PhylomeDB	Q9K971.
ProtClustDB	PRK05444.
Enzyme and pathway databases
BioCyc	BHAL272558:BH2779-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR011766. TPP_enzyme-bd_C. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_BACHD

Accession

Primary (citable) accession number: Q9K971

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program