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Protein

Oligo-1,6-glucosidase

Gene

malL

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei199 – 1991NucleophileBy similarity
Active sitei256 – 2561Proton donorBy similarity
Sitei330 – 3301Transition state stabilizerBy similarity

GO - Molecular functioni

  1. cation binding Source: InterPro
  2. oligo-1,6-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciBHAL272558:GJC5-2999-MONOMER.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Oligo-1,6-glucosidase (EC:3.2.1.10)
Alternative name(s):
Dextrin 6-alpha-D-glucanohydrolase
Oligosaccharide alpha-1,6-glucosidase
Sucrase-isomaltase
Short name:
Isomaltase
Gene namesi
Name:malL
Ordered Locus Names:BH2903
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001258 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 561561Oligo-1,6-glucosidasePRO_0000054315Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272558.BH2903.

Structurei

3D structure databases

ProteinModelPortaliQ9K8U9.
SMRiQ9K8U9. Positions 1-556.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

eggNOGiCOG0366.
HOGENOMiHOG000220641.
KOiK01182.
OMAiNAPEEWP.
OrthoDBiEOG6RVFV2.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9K8U9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTQWWKESV VYQIYPRSFQ DYNGDGIGDI PGIISRLDYL KTLGVDVIWL
60 70 80 90 100
SPVYDSPNDD NGYDIRDYKA IMDEFGTMAD WETLLAEIHT RGMKLIMDLV
110 120 130 140 150
VNHSSDEHAW FVESRKSKDN PYRDFYIWRP GKDGKEPNNW ASNFSGSAWT
160 170 180 190 200
YDETTGEYYL HLFSKKQPDL NWENPKLREK IYEMMTWWLD KGIDGFRMDV
210 220 230 240 250
INFISKVDGL PDAEPQPGQP YVSGSNYFMN GPNIHTYLQE MHENVLQHYD
260 270 280 290 300
LMTVGEMPGV TLELAQLYTG EERNELNMVF QFEHVGLDQG PNGKWDLKPL
310 320 330 340 350
ELKDLKASLS RWQKGLQDIG WNSLYWNNHD QPRIVSRFGD DQSYRVESAK
360 370 380 390 400
MLATLLHCMK GTPFIYQGEE IGMTNVRFDS IEQYQDIETL NMYKEKRAQG
410 420 430 440 450
VPHETLMASI HAKGRDNART PMQWDETKHG GFTDGTPWLE VNPNYKEINV
460 470 480 490 500
KQALKDPNSI FYHYQKLIQL RKEHAILVHG SYDLILEDDP EIFAYKRTYN
510 520 530 540 550
GQTLLVVCNF YGRITDFECP AEVVLSEPTL LLSNYDEEEN GSYTSFRLRP
560
YEARVYLGKN E
Length:561
Mass (Da):65,430
Last modified:October 1, 2000 - v1
Checksum:i82C6F3E09F18D4F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB06622.1.
PIRiG84012.
RefSeqiNP_243769.1. NC_002570.2.
WP_010899050.1. NC_002570.2.

Genome annotation databases

EnsemblBacteriaiBAB06622; BAB06622; BAB06622.
KEGGibha:BH2903.
PATRICi18943038. VBIBacHal18977_3020.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB06622.1.
PIRiG84012.
RefSeqiNP_243769.1. NC_002570.2.
WP_010899050.1. NC_002570.2.

3D structure databases

ProteinModelPortaliQ9K8U9.
SMRiQ9K8U9. Positions 1-556.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272558.BH2903.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB06622; BAB06622; BAB06622.
KEGGibha:BH2903.
PATRICi18943038. VBIBacHal18977_3020.

Phylogenomic databases

eggNOGiCOG0366.
HOGENOMiHOG000220641.
KOiK01182.
OMAiNAPEEWP.
OrthoDBiEOG6RVFV2.

Enzyme and pathway databases

BioCyciBHAL272558:GJC5-2999-MONOMER.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis."
    Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.
    Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Entry informationi

Entry nameiO16G_BACHD
AccessioniPrimary (citable) accession number: Q9K8U9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: October 1, 2000
Last modified: April 29, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.