Q9K8U9 (O16G_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Oligo-1,6-glucosidase
EC=3.2.1.10

Alternative name(s):
Dextrin 6-alpha-D-glucanohydrolase
Oligosaccharide alpha-1,6-glucosidase
Sucrase-isomaltase
Short name=Isomaltase

Gene names

Name:	malL
Ordered Locus Names:	BH2903

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus halodurans

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cation binding Inferred from electronic annotation. Source: InterPro oligo-1,6-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	Oligo-1,6-glucosidase		PRO_0000054315
Sites
	Active site	199	1	Nucleophile By similarity
	Active site	256	1	Proton donor By similarity
	Site	330	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9K8U9 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 82C6F3E09F18D4F7	FASTA	561	65,430
	10 20 30 40 50 60 MSTQWWKESV VYQIYPRSFQ DYNGDGIGDI PGIISRLDYL KTLGVDVIWL SPVYDSPNDD 70 80 90 100 110 120 NGYDIRDYKA IMDEFGTMAD WETLLAEIHT RGMKLIMDLV VNHSSDEHAW FVESRKSKDN 130 140 150 160 170 180 PYRDFYIWRP GKDGKEPNNW ASNFSGSAWT YDETTGEYYL HLFSKKQPDL NWENPKLREK 190 200 210 220 230 240 IYEMMTWWLD KGIDGFRMDV INFISKVDGL PDAEPQPGQP YVSGSNYFMN GPNIHTYLQE 250 260 270 280 290 300 MHENVLQHYD LMTVGEMPGV TLELAQLYTG EERNELNMVF QFEHVGLDQG PNGKWDLKPL 310 320 330 340 350 360 ELKDLKASLS RWQKGLQDIG WNSLYWNNHD QPRIVSRFGD DQSYRVESAK MLATLLHCMK 370 380 390 400 410 420 GTPFIYQGEE IGMTNVRFDS IEQYQDIETL NMYKEKRAQG VPHETLMASI HAKGRDNART 430 440 450 460 470 480 PMQWDETKHG GFTDGTPWLE VNPNYKEINV KQALKDPNSI FYHYQKLIQL RKEHAILVHG 490 500 510 520 530 540 SYDLILEDDP EIFAYKRTYN GQTLLVVCNF YGRITDFECP AEVVLSEPTL LLSNYDEEEN 550 560 GSYTSFRLRP YEARVYLGKN E

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB06622.1.
PIR	G84012.
RefSeq	NP_243769.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9K8U9.
SMR	Q9K8U9. Positions 1-556.
ModBase	Search...
Protein-protein interaction databases
STRING	272558.BH2903.
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB06622; BAB06622; BAB06622.
GeneID	893083.
KEGG	bha:BH2903.
PATRIC	18943038. VBIBacHal18977_3020.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0366.
HOGENOM	HOG000220641.
KO	K01182.
OMA	IRTNGAT.
Enzyme and pathway databases
BioCyc	BHAL272558:GJC5-2999-MONOMER.
Family and domain databases
Gene3D	2.60.40.1180. 1 hit. 3.20.20.80. 2 hits.
InterPro	IPR015902. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR006589. Glyco_hydro_13_sub_cat_dom. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR10357. PTHR10357. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. [Graphical view]
SMART	SM00642. Aamy. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

O16G_BACHD

Accession

Primary (citable) accession number: Q9K8U9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 24, 2001
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program