Q9K8G2 (HEM2_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Delta-aminolevulinic acid dehydratase
Short name=ALAD
Short name=ALADH
EC=4.2.1.24

Alternative name(s):
Porphobilinogen synthase

Gene names

Name:	hemB
Ordered Locus Names:	BH3044

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus halodurans

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.
Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O.
Cofactor	Binds 1 zinc ion per monomer By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
Subunit structure	Homooctamer By similarity.
Sequence similarities	Belongs to the ALADH family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Ligand	Magnesium Metal-binding Zinc
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW porphobilinogen synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Delta-aminolevulinic acid dehydratase		PRO_0000140492
Sites
	Active site	197	1	Schiff-base intermediate with substrate By similarity
	Active site	250	1	Schiff-base intermediate with substrate By similarity
	Metal binding	122	1	Zinc; catalytic By similarity
	Metal binding	124	1	Zinc; catalytic By similarity
	Metal binding	132	1	Zinc; catalytic By similarity
	Metal binding	235	1	Magnesium By similarity
	Binding site	207	1	Substrate 1 By similarity
	Binding site	219	1	Substrate 1 By similarity
	Binding site	276	1	Substrate 2 By similarity
	Binding site	315	1	Substrate 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9K8G2 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 74BDEEE1C95B1646	FASTA	328	36,166
	10 20 30 40 50 60 MGQDLTFKRH RRLRQTAAIR NMVRETKLHI DDLIYPIFVK EGEGVRQEVP SMPNVYQLSL 70 80 90 100 110 120 DQLDAEVDEI VSLGIPAIIL FGVPAEKDAV GSAAYHEHGI VQQAIRQVKE SYPDLTVIAD 130 140 150 160 170 180 TCLCQFTDHG HCGVIEEGKI LNDPSLDLLA RTAVSQAKAG ADIIAPSNMM DGFVAAIRAG 190 200 210 220 230 240 LDSAGFTDVP VMSYAVKYAS AFYGPFRDAA HSSPVFGDRK TYQMDPANRL EALREARSDV 250 260 270 280 290 300 EEGADFLIVK PALSYLDIIR EVKNETGLPV VAYNVSGEYS MIKAASLQGW IDEKSTVLEK 310 320 LISMKRAGAD LILTYFAKDV ARWLNEEK

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB06763.1.
PIR	D84030.
RefSeq	NP_243910.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9K8G2.
SMR	Q9K8G2. Positions 7-322.
ModBase	Search...
Protein-protein interaction databases
STRING	272558.BH3044.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB06763; BAB06763; BAB06763.
GeneID	890913.
KEGG	bha:BH3044.
PATRIC	18943328. VBIBacHal18977_3165.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0113.
HOGENOM	HOG000020323.
KO	K01698.
OMA	MIISYHA.
ProtClustDB	PRK09283.
Enzyme and pathway databases
BioCyc	BHAL272558:GJC5-3141-MONOMER.
UniPathway	UPA00251; UER00318.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR001731. Porphobilinogen_synth. [Graphical view]
PANTHER	PTHR11458. PTHR11458. 1 hit.
Pfam	PF00490. ALAD. 1 hit. [Graphical view]
PIRSF	PIRSF001415. Porphbilin_synth. 1 hit.
PRINTS	PR00144. DALDHYDRTASE.
SMART	SM01004. ALAD. 1 hit. [Graphical view]
PROSITE	PS00169. D_ALA_DEHYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM2_BACHD

Accession

Primary (citable) accession number: Q9K8G2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program