Reviewed, UniProtKB/Swiss-Prot Q9K8E7 (ILVC_BACHD)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	BH3059

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000151276
Sites
	Active site	107	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9K8E7-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 2EA0D40B5E07B666	FASTA	340	37,071
	10 20 30 40 50 60 MAKVFYNGDI NEGVLQGKTV AIIGYGSQGH AHAQNLRDSG QEVIVGLRPG KSWDKAAEDG 70 80 90 100 110 120 FQVYSVREAA SRADVIMILL PDEHQPTVYK NEIEPELSEG KTLAFAHGFN VHFNQIVPPA 130 140 150 160 170 180 TVDVFLAAPK GPGHLVRRTY VDGAGVPGLV AVYQDATGQA KDIALAYSKM NGSARAGVIE 190 200 210 220 230 240 TTFQEETETD LFGEQAVLCG GTSALVKAGF ETLVEAGYQP EVAYFECLHE LKLIVDLMYE 250 260 270 280 290 300 GGLEYMRYSI SDTAQWGDFQ AGPRVVTAET KQAMKDILSD IQTGKFAKGW ILENQANRPE 310 320 330 340 FTAINEREKN HPLEVVGREL REMMPFVKAK SKGVVGSAKN

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000004 Genomic DNA. Translation: BAB06778.1.
PIR	C84032.
RefSeq	NP_243925.1.
3D structure databases
HSSP	HSSP built from PDB template 1NP3 based on UniProtKB Q9HVA2.
ModBase	Search...
Genome annotation databases
GeneID	890538.
GenomeReviews	Gene locus BH3059 in contig BA000004_GR.
KEGG	bha:BH3059.
NMPDR	fig|272558.1.peg.3059.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9K8E7.
OMA	Q9K8E7. AHGFNIR.
Enzyme and pathway databases
BioCyc	BHAL272558:BH3059-MON.
BRENDA	1.1.1.86. 191865.
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_BACHD

Accession

Primary (citable) accession number: Q9K8E7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)