Reviewed, UniProtKB/Swiss-Prot Q9K8E4 (ILVD_BACHD)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase
      Short name=DAD
    EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	BH3062

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000103430
Sites
	Metal binding	119	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	192	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9K8E4-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E2934611EFB864AE	FASTA	555	59,810
	10 20 30 40 50 60 MRSDMIKKGI DRAPHRSLLR AAGVKEEEMD KPFIGVCNSY IDIIPGHMHL NKFAEVAKEA 70 80 90 100 110 120 IREAGGIPFE FNTIGVDDGI AMGHIGMRYS LPSREIICDA AETVINAHWF DGVFFIPNCD 130 140 150 160 170 180 KITPGMLMAS VRTNVPSVFV SGGPMEAGRT KDGKNLSLAS VFEGVGAFSS GKMTREELLE 190 200 210 220 230 240 IEQTACPTCG SCSGMFTANS MNSLMEMLGM ALPGNGTIVA TSEARHQLIK DAAKHLMNLI 250 260 270 280 290 300 EKDIRPRDII TKETIDDAFA LDMAMGGSTN TVLHTLAIAN EAEIEYDLNR INEVAERVPY 310 320 330 340 350 360 LCKISPASDY SMDDVHHAGG VAAIIKELCE IDGAIHPDRI TITGKSIYEN VKDAEITDDV 370 380 390 400 410 420 VIRRKDNPYS PVGGLSILFG NLAPNGAVIK VGAVDPSIQI FEGEAIVYNS QEEAQQGINN 430 440 450 460 470 480 GDVREGHVVV IRYEGPKGGP GMPEMLAPTS AIIGRGLGTK VALITDGRFS GASRGISIGH 490 500 510 520 530 540 ISPEAAEGGP IAFIENGDKI RIDLPNRTIE WLVSDEEIAK RQEGWTEPEP KVKKGYLARY 550 SKLVTSANTG GVMKI

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000004 Genomic DNA. Translation: BAB06781.1.
PIR	F84032.
RefSeq	NP_243928.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	891012.
GenomeReviews	Gene locus BH3062 in contig BA000004_GR.
KEGG	bha:BH3062.
NMPDR	fig|272558.1.peg.3062.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9K8E4.
OMA	SRKVPCL.
Enzyme and pathway databases
BioCyc	BHAL272558:BH3062-MON.
BRENDA	4.2.1.9. 191865.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BACHD

Accession

Primary (citable) accession number: Q9K8E4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)