Q9K8D9 (NTPA_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

BH3067

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 194	194	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178125
Regions
	Region	8 – 13	6	Substrate binding By similarity
	Region	70 – 71	2	Substrate binding By similarity
Sites
	Metal binding	41	1	Magnesium or manganese By similarity
	Metal binding	70	1	Magnesium or manganese By similarity
	Binding site	156	1	Substrate By similarity
	Binding site	176	1	Substrate By similarity
	Binding site	182	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9K8D9 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: BBC5A0C4F19A04B3	FASTA	194	21,868
	10 20 30 40 50 60 MKEWIVATKN KGKVAEFEAI LGKRGFSVKS LLDYPAIEDI EETGSTFNEN ATIKAEAIAE 70 80 90 100 110 120 RFQRPVLADD SGLIIDALDG RPGIFSARYA GEEKDDQKNI EKVLRELQDI PWKARTARFH 130 140 150 160 170 180 CSIALARPQA ETIVFEGTCE GYITTEPKGT GGFGYDPIFY VPSHDKTMAE LTQEEKNKLS 190 HRYHALKQLD KWLD

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB06786.1.
PIR	C84033.
RefSeq	NP_243933.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9K8D9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000053268; EBBACP00000051907; EBBACG00000053259.
GeneID	890587.
GenomeReviews	Gene locus BH3067 in contig BA000004_GR.
KEGG	bha:BH3067.
NMPDR	fig|272558.1.peg.3067.
PATRIC	18943386. VBIBacHal18977_3192.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000001334.
HOGENOM	HBG697237.
OMA	GVYTADW.
PhylomeDB	Q9K8D9.
ProtClustDB	PRK14822.
Enzyme and pathway databases
BioCyc	BHAL272558:BH3067-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_BACHD

Accession

Primary (citable) accession number: Q9K8D9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program