Q9K8D8 (RNPH_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	BH3068

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	tRNA binding Inferred from electronic annotation. Source: InterPro tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: EC tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	Ribonuclease PH HAMAP MF_00564		PRO_0000139866

Sequences

	Sequence		Length	Mass (Da)
	Q9K8D8 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: D6F83C157718FB28	FASTA	257	28,267
	10 20 30 40 50 60 MRMDERKADE LRPISIEINY LHHPEGSVLI SIGNTKVICT ASLEDRVPPF LRGQGKGWIT 70 80 90 100 110 120 AEYSMLPRAT EQRNMRESTR GKVSGRTMEI QRLIGRALRS IIDLDKIGER TVWIDCDVIQ 130 140 150 160 170 180 ADGGTRTTSI TGAFVALSLA MEKALANGAI QEWPIDDFLA AVSVGIDPKL GAILDLCYAE 190 200 210 220 230 240 DAQAEVDMNV VMTGKGEYVE LQGTGEEATF SHNQLLTILA LADKGIKQLI EEQRNALGAI 250 ANRVDEAKQK KKEQSEA

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB06787.1.
PIR	D84033.
RefSeq	NP_243934.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9K8D8.
SMR	Q9K8D8. Positions 1-238.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000050758; EBBACP00000049397; EBBACG00000050749.
GeneID	890838.
GenomeReviews	Gene locus BH3068 in contig BA000004_GR.
KEGG	bha:BH3068.
NMPDR	fig|272558.1.peg.3068.
PATRIC	18943388. VBIBacHal18977_3193.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000001825.
HOGENOM	HBG737187.
OMA	MLPRATG.
PhylomeDB	Q9K8D8.
ProtClustDB	PRK00173.
Enzyme and pathway databases
BioCyc	BHAL272558:BH3068-MONOMER.
Family and domain databases
HAMAP	MF_00564. RNase_PH. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
KO	K00989.
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_BACHD

Accession

Primary (citable) accession number: Q9K8D8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program