Reviewed, UniProtKB/Swiss-Prot Q9K7X9 (SYY_BACHD)

Names and origin

Protein names

Recommended name:
    Tyrosyl-tRNA synthetase
    EC=6.1.1.1
Alternative name(s):
    Tyrosine--tRNA ligase
      Short name=TyrRS

Gene names

Name:	tyrS
Ordered Locus Names:	BH3228

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity.
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02007
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	Tyrosyl-tRNA synthetase HAMAP MF_02007		PRO_0000236693
Regions
	Domain	351 – 412	62	S4 RNA-binding
	Motif	56 – 65	10	"HIGH" region HAMAP MF_02007
	Motif	240 – 244	5	"KMSKS" region HAMAP MF_02007
Sites
	Binding site	243	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9K7X9-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 913C7A9A98FE02E6	FASTA	412	46,724
	10 20 30 40 50 60 MEEKALTPEQ TQEVERQLEI LKRGVVEIVP EEALREKIEK SVRTGKPLKV KLGMDPSAPD 70 80 90 100 110 120 VHIGHTVVLH KLRQFQDLGH EVQMLIGDFT GRIGDPSGKS ETRKQLTDEQ VKANARTYVE 130 140 150 160 170 180 QYGKILDMEK ATLHYNSKWL SALNFEDVIK LASQMTVARM LERDDFEKRY KSGQPISIHE 190 200 210 220 230 240 FFYPLMQGYD SVALESDIEI GGTDQKFNLL MGRMLQEAYG NDKQVAITMP LIEGLDGERK 250 260 270 280 290 300 MSKSLNNYIG IDEAPNEIFG KAMSIPDELM VKYYELATDL SMDEVKAIAK GLEDGSVHPR 310 320 330 340 350 360 DAKMKLGATL VRMYHGEKEA EEAVNYFKTV FQKRDLPTDI PEVKWDGESP VWIVDLLVTL 370 380 390 400 410 DLLPSKGEAR RMVQNGGVKL NGEKVEDVQL QVEIEDGLIV QVGKRKFKKL VR

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000004 Genomic DNA. Translation: BAB06947.1.
PIR	D84053.
RefSeq	NP_244094.1.
3D structure databases
HSSP	HSSP built from PDB template 1H3F based on UniProtKB P83453.
ModBase	Search...
Genome annotation databases
GeneID	890495.
GenomeReviews	Gene locus BH3228 in contig BA000004_GR.
KEGG	bha:BH3228.
NMPDR	fig|272558.1.peg.3228.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9K7X9.
OMA	YVVQVGK.
Enzyme and pathway databases
BioCyc	BHAL272558:BH3228-MON.
BRENDA	6.1.1.1. 191865.
Family and domain databases
HAMAP	MF_02007. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ib. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA_bd. IPR002307. Tyr-tRNA-synth_Ib_bac/mito. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF01479. S4. 1 hit. PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY_BACHD

Accession

Primary (citable) accession number: Q9K7X9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)