Reviewed, UniProtKB/Swiss-Prot Q9K7W1 (MURC_BACHD)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	BH3248

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000182051
Regions
	Nucleotide binding	108 – 114	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9K7W1-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: A5E6962FCA4760B7	FASTA	433	48,657
	10 20 30 40 50 60 MTTFHFIGIK GSGMSALAQV LHDMNYQVQG SDVEKRFFTQ KPLEEKGIPI FPFQKENIKE 70 80 90 100 110 120 GQQVVVSAAY SDEHEEVVAA KEQGLEIQSY PTFLGEFIQR FTSIAVTGSH GKTSTTGLLS 130 140 150 160 170 180 HVLGSHCPTS FLIGDGTGKG AADSHYFVFE ACEYRRHFLN YRPDYCIMTN IDFDHPDYFD 190 200 210 220 230 240 DINDVFSAFQ EMAMQVKKAI VACGDDEQLQ GIHANVPVVY YGFNEDNDFQ ARNIEVTSEG 250 260 270 280 290 300 THFQVFVRNN HYGDFTIPRF GKHNVLNSLA VIALCHYENI PVEVLARELK TFEGVKRRFT 310 320 330 340 350 360 EKTLGGQILI DDYAHHPTEI SATIEAAKQK YPDRPVVAIF QPHTFSRTKA FLHEFAEALK 370 380 390 400 410 420 QADHVYLCDI FGSARENKGS LSIEDLQALI PGAHLLSEND LAPLQDHRSG VLLFMGAGDI 430 QKFQQAYEHV LTT

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000004 Genomic DNA. Translation: BAB06967.1.
PIR	H84055.
RefSeq	NP_244114.1.
3D structure databases
HSSP	HSSP built from PDB template 1GQQ based on UniProtKB P45066.
ModBase	Search...
Genome annotation databases
GeneID	891104.
GenomeReviews	Gene locus BH3248 in contig BA000004_GR.
KEGG	bha:BH3248.
NMPDR	fig|272558.1.peg.3248.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9K7W1.
OMA	DTHEEIE.
Enzyme and pathway databases
BioCyc	BHAL272558:BH3248-MON.
BRENDA	6.3.2.8. 191865.
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_BACHD

Accession

Primary (citable) accession number: Q9K7W1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)