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Q9K7S8 (SYL_BACHD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:BH3281
OrganismBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]
Taxonomic identifier272558 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length806 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 806806Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000151969

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif576 – 5805"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9K7S8 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: C019DA99B555F479

FASTA80692,066
        10         20         30         40         50         60 
MSFSHREIES KWQKYWEENK TFKTEEDETR EKFYALDMFP YPSGAGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
LSRMKRMQGY NVLHPMGWDA FGLPAEQYAI DTGNSPAEFT EKNINTFRRQ IKSLGFSYDW 

       130        140        150        160        170        180 
DREVNTTDPD YYKWTQWIFI QLYNKGLAYI DEVAVNWCPA LGTVLANEEV IDGKSERGGH 

       190        200        210        220        230        240 
PVERRPMKQW MLKITEYADR LLEDLEELDW PESIKDMQRN WIGRSEGAEV TFSVDGHDDT 

       250        260        270        280        290        300 
ITVFTTRPDT LFGATYMVLA PEHKLVDAIT TSEQKQAVES YKKEVATKSD LERTELAKEK 

       310        320        330        340        350        360 
TGVFTGAYAI NPVNGEKVPI WIADYVLVSY GTGAIMAVPA HDERDYEFAK TFDLPIKEVV 

       370        380        390        400        410        420 
SGGVIEQEAY TGDGPHVNSE FLNGLSKEEA IEKMIQWLEA EKKGTKKVTY RLRDWLFSRQ 

       430        440        450        460        470        480 
RYWGEPIPVI HWEDGTMSTV PEDELPLELP KMSEIKPSGT GESPLANATD WLEVVDPVTG 

       490        500        510        520        530        540 
KKGRRETNTM PQWAGSCWYY LRYIDPDNER MIADPEKLKK WLPVDIYIGG AEHAVLHLLY 

       550        560        570        580        590        600 
ARFWHKVLYD LGVVPTKEPF QKLYNQGMIL GENNEKMSKS KGNVVNPDDI IDSHGADTLR 

       610        620        630        640        650        660 
LYEMFMGPLD ASIAWSTTGL DGARRFLDRV WRLLVDENTE EKSSKIVNGE GSPELKRAYH 

       670        680        690        700        710        720 
QTVKKVTEDF EELRFNVGIS QLMVYVNEAY KQEELPLDQA EGFVKLLSPV APHLAEELWS 

       730        740        750        760        770        780 
KLGHEGTIAY EPWPTYDEAF LVEDEVEIVV QHNGKVRAKV VVAKDATKEQ MEEAALANER 

       790        800 
VKESIDGKTV RKVIVVPGKL VNIVAN 

« Hide

References

[1]"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis."
Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.
Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000004 Genomic DNA. Translation: BAB07000.1.
PIRA84060.
RefSeqNP_244147.1. NC_002570.2.

3D structure databases

ProteinModelPortalQ9K7S8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272558.BH3281.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB07000; BAB07000; BAB07000.
GeneID891254.
KEGGbha:BH3281.
PATRIC18943836. VBIBacHal18977_3417.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycBHAL272558:GJC5-3380-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BACHD
AccessionPrimary (citable) accession number: Q9K7S8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries