Q9K7R6 (HDOX_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heme-degrading monooxygenase
EC=1.14.99.3

Alternative name(s):
Heme oxygenase
Iron-regulated surface determinant
Iron-responsive surface determinant

Gene names

Name:	isdG
Ordered Locus Names:	BH3293

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus halodurans

Protein attributes

Sequence length	116 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows bacterial pathogens to use the host heme as an iron source. Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring to the biliverdin in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron By similarity. HAMAP-Rule MF_01272
Catalytic activity	Heme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O. HAMAP-Rule MF_01272
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the antibiotic biosynthesis monooxygenase family. Heme-degrading monooxygenase IsdG subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	heme catabolic process Inferred from electronic annotation. Source: HAMAP iron assimilation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	heme binding Inferred from electronic annotation. Source: HAMAP heme oxygenase (decyclizing) activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 116	116	Heme-degrading monooxygenase HAMAP-Rule MF_01272		PRO_0000270075
Sites
	Metal binding	6	1	Iron By similarity
	Metal binding	76	1	Iron (heme axial ligand) By similarity
	Site	66	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9K7R6 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 87F6CFB28A691023	FASTA	116	13,175
	10 20 30 40 50 60 MVIVTNTSKI TKGNGEKLIE RFNKVGKVEF MEGFLGLEVL LTENTKDFDE VTVVTRWNTK 70 80 90 100 110 DDFKNWTKSS AFRDAHSKRE VPEYILENKI SFYEVKVVRG PLTAAEAGND SQAQAQ

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB07012.1.
PIR	E84061.
RefSeq	NP_244159.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9K7R6.
ModBase	Search...
Protein-protein interaction databases
STRING	272558.BH3293.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB07012; BAB07012; BAB07012.
GeneID	891123.
KEGG	bha:BH3293.
PATRIC	18943862. VBIBacHal18977_3430.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2329.
HOGENOM	HOG000008026.
KO	K07145.
OMA	VTNTSKI.
ProtClustDB	PRK13314.
Enzyme and pathway databases
BioCyc	BHAL272558:GJC5-3392-MONOMER.
Family and domain databases
HAMAP	MF_01272. Heme_degrading_monooxygenase.
InterPro	IPR007138. Antibiotic_mOase. IPR011008. Dimeric_a/b-barrel. IPR023953. Heme-degrad_mOase. [Graphical view]
Pfam	PF03992. ABM. 1 hit. [Graphical view]
SUPFAM	SSF54909. Dimer_A_B_barrel. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HDOX_BACHD

Accession

Primary (citable) accession number: Q9K7R6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program