Reviewed, UniProtKB/Swiss-Prot Q9K7E4 (KHSE_BACHD)

Names and origin

Protein names

Recommended name:
    Homoserine kinase
      Short name=HSK
      Short name=HK
    EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	BH3420

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro threonine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP homoserine kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Homoserine kinase HAMAP MF_00384		PRO_0000156551
Regions
	Nucleotide binding	88 – 98	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9K7E4-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: B596B44691D2164E	FASTA	309	33,451
	10 20 30 40 50 60 MTLPFQITVP GSSANLGPGF DSVGLAVNRY LTLTVTEGSE WHFSTQSADL VGIPSGKENL 70 80 90 100 110 120 VYQVAEHVAS QLEKTLPPCH VQMESNIPLA RGLGSSAAAI VAGIELANQL LGQPLAAEDK 130 140 150 160 170 180 VRFGSLWEGH PDNIAPSVYG GLVIGTHLST ETHVIHGGVP DLDLVLLVPK EELLTKKARG 190 200 210 220 230 240 ILPESLSYKE AVRGSSVSNV LVAALLKENW ELVGEMMVRD VFHHPYRLGL VPHLQEVIRY 250 260 270 280 290 300 VKEETEAYGA ALSGAGPTML CLSPKGRGEW VQKQLQKQYP QFEVDVLKPD DQGIQVHRVL SNQQQLPIG

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000004 Genomic DNA. Translation: BAB07139.1.
PIR	D84077.
RefSeq	NP_244287.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	894385.
GenomeReviews	Gene locus BH3420 in contig BA000004_GR.
KEGG	bha:BH3420.
NMPDR	fig|272558.1.peg.3420.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9K7E4.
OMA	FDCLGAA.
Enzyme and pathway databases
BioCyc	BHAL272558:BH3420-MON.
BRENDA	2.7.1.39. 191865.
Family and domain databases
HAMAP	MF_00384. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP_bd_CS. IPR000870. Homoserine_kin. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
TIGRFAMs	TIGR00191. thrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_BACHD

Accession

Primary (citable) accession number: Q9K7E4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)