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Protein

Lipoyl synthase

Gene

lipA

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.

Catalytic activityi

Protein N6-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = protein N6-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.

Cofactori

[4Fe-4S] clusterNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]. This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi40Iron-sulfur 1 (4Fe-4S)1
Metal bindingi45Iron-sulfur 1 (4Fe-4S)1
Metal bindingi51Iron-sulfur 1 (4Fe-4S)1
Metal bindingi67Iron-sulfur 2 (4Fe-4S-S-AdoMet)1
Metal bindingi71Iron-sulfur 2 (4Fe-4S-S-AdoMet)1
Metal bindingi74Iron-sulfur 2 (4Fe-4S-S-AdoMet)1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:BH3435
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001258 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001022871 – 303Lipoyl synthaseAdd BLAST303

Interactioni

Protein-protein interaction databases

STRINGi272558.BH3435.

Structurei

3D structure databases

ProteinModelPortaliQ9K7C9.
SMRiQ9K7C9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Phylogenomic databases

eggNOGiENOG4105C0G. Bacteria.
COG0320. LUCA.
HOGENOMiHOG000235998.
KOiK03644.
OMAiPYCDIDF.
OrthoDBiPOG091H069D.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR031691. LIAS_N.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
PfamiView protein in Pfam
PF16881. LIAS_N. 1 hit.
PF04055. Radical_SAM. 1 hit.
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SFLDiSFLDG01058. lipoyl_synthase_like. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
SMARTiView protein in SMART
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9K7C9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKKEEYVRK PDWLKIKLNT NETYTGLKKM MREKNLHTVC EEAKCPNIHE
60 70 80 90 100
CWAVRKTATF MILGDVCTRA CRFCAVKTGL PNELDLQEPE RVAESVEIMG
110 120 130 140 150
LKHAVITAVA RDDLKDGGAG VFAETVRAVR RRNPFCTIEV LPSDMMGNDD
160 170 180 190 200
NLKTLMDARP NILNHNIETV RRLTPRVRAR ATYERSLEFL RRAKEMQPDI
210 220 230 240 250
PTKSSLMIGL GETKDEIIET MDDLRANNVD IMTIGQYLQP TKKHLKVQKY
260 270 280 290 300
YHPDEFAELK EIALSKGFSH CEAGPLVRSS YHADEQVNEA QVREEARKAA

AKS
Length:303
Mass (Da):34,533
Last modified:October 1, 2000 - v1
Checksum:i0DF7334D2703A7D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB07154.1.
PIRiC84079.

Genome annotation databases

EnsemblBacteriaiBAB07154; BAB07154; BAB07154.
KEGGibha:BH3435.

Similar proteinsi

Entry informationi

Entry nameiLIPA_BACHD
AccessioniPrimary (citable) accession number: Q9K7C9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: October 1, 2000
Last modified: October 25, 2017
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families