Q9K6B9 (SPEB_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Agmatinase
EC=3.5.3.11

Alternative name(s):
Agmatine ureohydrolase
Short name=AUH

Gene names

Name:	speB
Ordered Locus Names:	BH3810

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus halodurans

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of putrescine from agmatine By similarity.
Catalytic activity	Agmatine + H2O = putrescine + urea.
Cofactor	Manganese By similarity.
Pathway	Amine and polyamine biosynthesis; putrescine biosynthesis via agmatine pathway; putrescine from agmatine: step 1/1.
Sequence similarities	Belongs to the arginase family. Agmatinase subfamily.
Sequence caution	The sequence BAB07529.1 differs from that shown. Reason: Frameshift at position 40.

Ontologies

Keywords
Biological process	Polyamine biosynthesis Putrescine biosynthesis Spermidine biosynthesis
Ligand	Manganese Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	putrescine biosynthetic process from arginine Inferred from electronic annotation. Source: UniProtKB-UniPathway spermidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	agmatinase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Agmatinase		PRO_0000173727
Sites
	Metal binding	112	1	Manganese By similarity
	Metal binding	135	1	Manganese By similarity
	Metal binding	137	1	Manganese By similarity
	Metal binding	139	1	Manganese By similarity
	Metal binding	216	1	Manganese By similarity
	Metal binding	218	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9K6B9 [UniParc]. Last modified March 15, 2004. Version 2. Checksum: 2F8B0C4CA4EB4B89	FASTA	289	32,155
	10 20 30 40 50 60 MRFDEAYSGK VFIMSRSNYE KSKAVIFGMP MDWTVSFRPS SRFGPNRIRE ASLGLEEYSP 70 80 90 100 110 120 YMDKHLEEVA YFDAGDMLLP FGNPQRSLEM IESYVDKLLA DQKMPIGLGG EHLVSWPIFK 130 140 150 160 170 180 AMHKIYPDMA IIHIDAHADL REEYEGEPLS HSTPIRKACS LIGPENVYSF GIRSGMREEF 190 200 210 220 230 240 QYAKDSGMYM AKFEVATPLK EVLPKLAGRN VYVTIDIDVL DPAFAPGTGT AEAGGISSKE 250 260 270 280 LLEAIVAIAH SDVNVIGADL VEVAPAYDPS EKTPIAASKF VREMLLGWV

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB07529.1. Frameshift.
PIR	B84126.
RefSeq	NP_244677.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9K6B9.
ModBase	Search...
Protein-protein interaction databases
STRING	272558.BH3810.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB07529; BAB07529; BAB07529.
GeneID	890481.
KEGG	bha:BH3810.
PATRIC	18944936. VBIBacHal18977_3966.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0010.
KO	K01480.
OMA	AKENGMH.
Enzyme and pathway databases
BioCyc	BHAL272558:GJC5-3913-MONOMER.
UniPathway	UPA00534; UER00287.
Family and domain databases
Gene3D	3.40.800.10. 1 hit.
InterPro	IPR005925. Agmatinase-rel. IPR006035. Ureohydrolase. IPR023696. Ureohydrolase_domain. IPR020855. Ureohydrolase_Mn_BS. [Graphical view]
PANTHER	PTHR11358. PTHR11358. 1 hit.
Pfam	PF00491. Arginase. 1 hit. [Graphical view]
PIRSF	PIRSF036979. Arginase. 1 hit.
TIGRFAMs	TIGR01230. agmatinase. 1 hit.
PROSITE	PS01053. ARGINASE_1. 1 hit. PS51409. ARGINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPEB_BACHD

Accession

Primary (citable) accession number: Q9K6B9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	March 15, 2004
Last modified:	May 1, 2013
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program