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Q9K678 (PTMCB_BACHD) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
PTS system mannitol-specific EIICB component
Alternative name(s):
EIICB-Mtl
Short name=EII-Mtl

Including the following 2 domains:

  1. Mannitol permease IIC component
    Alternative name(s):
    PTS system mannitol-specific EIIC component
  2. Mannitol-specific phosphotransferase enzyme IIB component
    EC=2.7.1.69
    Alternative name(s):
    PTS system mannitol-specific EIIB component
Gene names
Name:mtlA
Ordered Locus Names:BH3854
OrganismBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]
Taxonomic identifier272558 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannitol transport By similarity.

Catalytic activity

Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity.

Domain

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

Sequence similarities

Contains 1 PTS EIIB type-2 domain.

Contains 1 PTS EIIC type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 468468PTS system mannitol-specific EIICB component
PRO_0000186608

Regions

Transmembrane20 – 4021Helical; Potential
Transmembrane50 – 7021Helical; Potential
Transmembrane93 – 11321Helical; Potential
Transmembrane137 – 15721Helical; Potential
Transmembrane163 – 18321Helical; Potential
Transmembrane215 – 23521Helical; Potential
Transmembrane269 – 28921Helical; Potential
Transmembrane316 – 33621Helical; Potential
Domain14 – 344331PTS EIIC type-2
Domain380 – 46889PTS EIIB type-2

Sites

Active site3861Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9K678 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 7B0FDA747E75DEB8

FASTA46849,038
        10         20         30         40         50         60 
MNNQPSFRAR VQKFGSFLSG MIMPNIGAFI AWGLITALFI PTGWWPNEQL AELVGPMITY 

        70         80         90        100        110        120 
LLPLLIGYTG GKMIYDVRGG VVGAAATMGV VVGADIPMFI GAMIMGPLGG FLIKKVDQVL 

       130        140        150        160        170        180 
QPKVRSGFEM LVNNFSAGIL AAILAIVAFL GIGPVVVSFS NVLASGVEVI IGAGLLPLAS 

       190        200        210        220        230        240 
IFIEPAKVLF LNNAINHGIL SPIGIDQAAS AGKSILFLLE TNPGPGLGVL LAFMVFGKGM 

       250        260        270        280        290        300 
AKQSAPGAAV IHFAGGIHEI YFPYILMKPT LILAVIAGGM SGVFTFVLFN AGLVAVPSPG 

       310        320        330        340        350        360 
SIFALLAMTP RGEYAGVLAG VIIATVVSFV IASIILKTSK ATAEDLTEAT SKMEGLKGKE 

       370        380        390        400        410        420 
SSVKEALITD DEQPQATEVN KIIFACDAGM GSSAMGASIL RDKVKKAGLS IEVANTSINQ 

       430        440        450        460 
LPDDVDIIIT HKDLTDRAKA KNPHAEHISV ENFLSSPKYD ELVNRLKS

« Hide

References

[1]"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis."
Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.
Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000004 Genomic DNA. Translation: BAB07573.1.
PIRF84131.
RefSeqNP_244721.1. NC_002570.2.

3D structure databases

ProteinModelPortalQ9K678.
SMRQ9K678. Positions 377-466.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000053985; EBBACP00000052624; EBBACG00000053976.
GeneID892459.
GenomeReviewsGene locus BH3854 in contig BA000004_GR.
KEGGbha:BH3854.
NMPDRfig|272558.1.peg.3854.
PATRIC18945026. VBIBacHal18977_4011.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000000035.
HOGENOMHBG296680.
OMAITHKDLT.
PhylomeDBQ9K678.

Enzyme and pathway databases

BioCycBHAL272558:BH3854-MONOMER.

Family and domain databases

InterProIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003352. PTS_EIIC.
IPR013014. PTS_EIIC_2.
IPR004718. PTS_IIC_mtl.
[Graphical view]
KOK02799.
K02800.
PfamPF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
TIGRFAMsTIGR00851. MtlA. 1 hit.
PROSITEPS51099. PTS_EIIB_TYPE_2. 1 hit.
PS51104. PTS_EIIC_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePTMCB_BACHD
AccessionPrimary (citable) accession number: Q9K678
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: October 1, 2000
Last modified: January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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