Reviewed, UniProtKB/Swiss-Prot Q9K5Z5 (ROCA2_BACHD)

Names and origin

Protein names

Recommended name:
    1-pyrroline-5-carboxylate dehydrogenase 2
      Short name=P5C dehydrogenase 2
    EC=1.5.1.12

Gene names

Name:	rocA2
Ordered Locus Names:	BH3940

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H. HAMAP MF_00733
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2. HAMAP MF_00733
Sequence similarities	Belongs to the aldehyde dehydrogenase family. RocA subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	1-pyrroline-5-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 515	515	1-pyrroline-5-carboxylate dehydrogenase 2 HAMAP MF_00733		PRO_0000056508
Sites
	Active site	286	1	By similarity
	Active site	320	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9K5Z5-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: A6742C0030D67C30	FASTA	515	56,308
	10 20 30 40 50 60 MLTPYKHEPF TDFSVEENRS AFEAALKNVE GELGKDYPLV INGERVTTDD KIVSVNPAMK 70 80 90 100 110 120 EQVIGVVSKA SREIVDDAFK SAETAFHTWK NVNPEERANI LIRAAAIIRR RKHEFSAWLV 130 140 150 160 170 180 KEAGKPWKEA DADTAEAIDF LEYYARQMIT LKDGKPVNSR EGEHNRYFYT PIGVCVTISP 190 200 210 220 230 240 WNFALAIMAG TTVAPIVTGN TVLLKPASTT PVVAAKFVEV LEEAGLPKGV VNFVPGSGTD 250 260 270 280 290 300 IGDYLIDHPK TSLITFTGSR DVGVRLYERA AVVHPGQQHL KRVIVEMGGK DTVVVDKDAD 310 320 330 340 350 360 LDLAAQSIVT SAFGFSGQKC SAGSRAVIHQ DVYDVVLEKA VALTKQLSVG EPTAPDVYMG 370 380 390 400 410 420 PVVDQGAFSK IMSYIEVGKE EGRLMVGGEG DDSKGFFIQP TIFADVDPHA RIMQEEIFGP 430 440 450 460 470 480 VVAFSKARDF DHALEIANNT EYGLTGAVIT TNRHHIEKAK RDFHVGNLYF NRNCTGAIVG 490 500 510 YHPFGGFKMS GTDSKAGGPD YLALHMQAKT VSEMY

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB07659.1.
PIR	D84142.
RefSeq	NP_244808.1.
3D structure databases
SMR	Q9K5Z5. Positions 2-515.
ModBase	Search...
Genome annotation databases
GeneID	891250.
GenomeReviews	Gene locus BH3940 in contig BA000004_GR.
KEGG	bha:BH3940.
NMPDR	fig|272558.1.peg.3940.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG752218.
OMA	TPYKHEP.
PhylomeDB	Q9K5Z5.
Enzyme and pathway databases
BioCyc	BHAL272558:BH3940-MONOMER.
BRENDA	1.5.1.12. 191865.
Family and domain databases
HAMAP	MF_00733. RocA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR005932. d-1-pyrroline-5-COlate_DH-2. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01237. D1pyr5carbox2. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ROCA2_BACHD

Accession

Primary (citable) accession number: Q9K5Z5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)