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Protein

Nitric oxide reductase transcription regulator NorR2

Gene

norR2

Organism
Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Required for the nitric oxide (NO) induced expression of NO reductase. Not required for expression of 2 other pathway members, nitrate reductase (nirS) and nitrous oxide reductase (nosZ).

Pathwayi: nitrate reduction (denitrification)

This protein regulates the pathway nitrate reduction (denitrification), which is part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the pathway nitrate reduction (denitrification) and in Nitrogen metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi221 – 228ATPPROSITE-ProRule annotation8
Nucleotide bindingi293 – 302ATPPROSITE-ProRule annotation10
DNA bindingi497 – 516H-T-H motifBy similarityAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00652.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide reductase transcription regulator NorR2
Gene namesi
Name:norR2
Ordered Locus Names:H16_B2325
OrganismiCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Taxonomic identifieri381666 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
Proteomesi
  • UP000008210 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000811621 – 521Nitric oxide reductase transcription regulator NorR2Add BLAST521

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei564-aspartylphosphateBy similarity1

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

By anaerobic growth in the presence of NO.

Interactioni

Protein-protein interaction databases

STRINGi381666.H16_B2325.

Structurei

3D structure databases

ProteinModelPortaliQ9K4U8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini193 – 422Sigma-54 factor interactionPROSITE-ProRule annotationAdd BLAST230

Sequence similaritiesi

Contains 1 sigma-54 factor interaction domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108JUA. Bacteria.
COG3604. LUCA.
HOGENOMiHOG000058487.
KOiK12266.
OMAiMGVSLYV.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.30.450.40. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003018. GAF.
IPR029016. GAF_dom-like.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view]
PfamiPF02954. HTH_8. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
PRINTSiPR01590. HTHFIS.
SMARTiSM00382. AAA. 1 hit.
SM00065. GAF. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55781. SSF55781. 1 hit.
PROSITEiPS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9K4U8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPSAMYPEL LADLVTDLPH AVRLQRLVSM LRTHFRCGAV ALLRLEEDHL
60 70 80 90 100
RPVAVDGLVR DALGRRFAVG LHPRLAAILA RRGVTCFHHD SMLPDPYDGL
110 120 130 140 150
IDEHVGEPLP VHDCMGTSLA VDGQPWGALT LDALAIGTFD AAAQAELQRL
160 170 180 190 200
TVIVEAAIRT TRLEGEIRAL QLARGTPEAD EGTAQHGDIG GEIIGQSEAI
210 220 230 240 250
ANLLHELEVV ADTDLPVLLL GETGVGKELF AHRLHRQSRR RAQPLVHVNC
260 270 280 290 300
AALPESLAES ELFGHARGAF SGATGERPGR FEAADGGTLF LDEVGELPLA
310 320 330 340 350
IQAKLLRTLQ NGEIQRLGSD RPRRVNVRVI AATNRNLREH VRDGSFRADL
360 370 380 390 400
YHRLSVYPIP IPPLRERGND VLLLAGRFLE LNRARLGLRS LRLSGGAQDA
410 420 430 440 450
LRSYRWPGNV RELEHVISRA ALRAVSRGAG RNDIVTLEPE LLDLDGLEVP
460 470 480 490 500
AAHHAGAGMA SAFAAPALAA GITLRDAVEQ TQRACIEQAL KDQGGNWAQA
510 520
ARQLGIDASN LHKLARRLGC K
Length:521
Mass (Da):56,494
Last modified:January 23, 2007 - v2
Checksum:iB3E4D73A8B6087B0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti124Q → T in CAC00712 (PubMed:11069685).Curated1
Sequence conflicti287 – 288GT → QP in CAC00712 (PubMed:11069685).Curated2
Sequence conflicti324 – 325RV → GS in CAC00712 (PubMed:11069685).Curated2
Sequence conflicti390S → T in CAC00712 (PubMed:11069685).Curated1
Sequence conflicti394 – 401SGGAQDAL → FRRCAGCV in CAC00712 (PubMed:11069685).Curated8
Sequence conflicti418 – 424SRAALRA → QPRGAAC in CAC00712 (PubMed:11069685).Curated7
Sequence conflicti460 – 464ASAFA → PARSH in CAC00712 (PubMed:11069685).Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278372 Genomic DNA. Translation: CAC00712.1.
AM260480 Genomic DNA. Translation: CAJ97107.1.
RefSeqiWP_011617790.1. NC_008314.1.

Genome annotation databases

EnsemblBacteriaiCAJ97107; CAJ97107; H16_B2325.
GeneIDi4456948.
KEGGireh:H16_B2325.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278372 Genomic DNA. Translation: CAC00712.1.
AM260480 Genomic DNA. Translation: CAJ97107.1.
RefSeqiWP_011617790.1. NC_008314.1.

3D structure databases

ProteinModelPortaliQ9K4U8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi381666.H16_B2325.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ97107; CAJ97107; H16_B2325.
GeneIDi4456948.
KEGGireh:H16_B2325.

Phylogenomic databases

eggNOGiENOG4108JUA. Bacteria.
COG3604. LUCA.
HOGENOMiHOG000058487.
KOiK12266.
OMAiMGVSLYV.

Enzyme and pathway databases

UniPathwayiUPA00652.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.30.450.40. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003018. GAF.
IPR029016. GAF_dom-like.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view]
PfamiPF02954. HTH_8. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
PRINTSiPR01590. HTHFIS.
SMARTiSM00382. AAA. 1 hit.
SM00065. GAF. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55781. SSF55781. 1 hit.
PROSITEiPS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNORR2_CUPNH
AccessioniPrimary (citable) accession number: Q9K4U8
Secondary accession number(s): Q0JYR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two very similar, functionally redundant regulators in this bacterium, NorR1 and NorR2.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.