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Q9K4A0 (DEF4_STRCO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peptide deformylase 4

Short name=PDF 4
EC=3.5.1.88
Alternative name(s):
Polypeptide deformylase 4
Gene names
Name:def4
Ordered Locus Names:SCO5221
ORF Names:SC7E4.18
OrganismStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) [Reference proteome] [HAMAP]
Taxonomic identifier100226 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length216 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity. HAMAP-Rule MF_00163

Catalytic activity

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP-Rule MF_00163

Cofactor

Binds 1 Fe2+ ion By similarity. HAMAP-Rule MF_00163

Sequence similarities

Belongs to the polypeptide deformylase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   LigandIron
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtranslation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functioniron ion binding

Inferred from electronic annotation. Source: InterPro

peptide deformylase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 216216Peptide deformylase 4 HAMAP-Rule MF_00163
PRO_0000082855

Sites

Active site1781 By similarity
Metal binding1351Iron By similarity
Metal binding1771Iron By similarity
Metal binding1811Iron By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9K4A0 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 562FDF5C60732AAF

FASTA21624,184
        10         20         30         40         50         60 
MAQQDTDQQH PGVLPVDDEG FVVDSQDCEE READWRGRGT SRPITVVGNP VLHKECEDVT 

        70         80         90        100        110        120 
DFGEEFQQLV ADMFASQRTA EGVGLAANQI GVSKKVFVYD CPDDEGVRHV GVVCNPRLVE 

       130        140        150        160        170        180 
LPADRRRLDD SNEGCLSVPT AYAPLARPDY AEVTGQDEKG NPVKVRGTGY FARCLQHETD 

       190        200        210 
HLYGYLYIDR LSKRERKDAL RQMAENEPRY PVVAND 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL939122 Genomic DNA. Translation: CAB94606.1.
RefSeqNP_629368.1. NC_003888.3.

3D structure databases

ProteinModelPortalQ9K4A0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING100226.SCO5221.

Proteomic databases

PRIDEQ9K4A0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB94606; CAB94606; CAB94606.
GeneID1100662.
KEGGsco:SCO5221.
PATRIC23740294. VBIStrCoe124346_5305.

Phylogenomic databases

eggNOGCOG0242.
HOGENOMHOG000243508.
KOK01462.
OMAHVGVVCN.
OrthoDBEOG664CMF.
PhylomeDBQ9K4A0.
ProtClustDBCLSK378634.

Family and domain databases

Gene3D3.90.45.10. 1 hit.
HAMAPMF_00163. Pep_deformylase.
InterProIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
PANTHERPTHR10458. PTHR10458. 1 hit.
PfamPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFPIRSF004749. Pep_def. 1 hit.
PRINTSPR01576. PDEFORMYLASE.
SUPFAMSSF56420. SSF56420. 1 hit.
TIGRFAMsTIGR00079. pept_deformyl. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDEF4_STRCO
AccessionPrimary (citable) accession number: Q9K4A0
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families