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Protein

Peptide deformylase 4

Gene

def4

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.UniRule annotation

Catalytic activityi

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi135 – 1351IronUniRule annotation
Metal bindingi177 – 1771IronUniRule annotation
Active sitei178 – 1781UniRule annotation
Metal bindingi181 – 1811IronUniRule annotation

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. peptide deformylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide deformylase 4UniRule annotation (EC:3.5.1.88UniRule annotation)
Short name:
PDF 4UniRule annotation
Alternative name(s):
Polypeptide deformylase 4UniRule annotation
Gene namesi
Name:def4UniRule annotation
Ordered Locus Names:SCO5221
ORF Names:SC7E4.18
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 216216Peptide deformylase 4PRO_0000082855Add
BLAST

Proteomic databases

PRIDEiQ9K4A0.

Interactioni

Protein-protein interaction databases

STRINGi100226.SCO5221.

Structurei

3D structure databases

ProteinModelPortaliQ9K4A0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the polypeptide deformylase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0242.
HOGENOMiHOG000243508.
InParanoidiQ9K4A0.
KOiK01462.
OMAiGYLYIDR.
OrthoDBiEOG664CMF.
PhylomeDBiQ9K4A0.

Family and domain databases

Gene3Di3.90.45.10. 1 hit.
HAMAPiMF_00163. Pep_deformylase.
InterProiIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
PANTHERiPTHR10458. PTHR10458. 1 hit.
PfamiPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFiPIRSF004749. Pep_def. 1 hit.
PRINTSiPR01576. PDEFORMYLASE.
SUPFAMiSSF56420. SSF56420. 1 hit.
TIGRFAMsiTIGR00079. pept_deformyl. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9K4A0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAQQDTDQQH PGVLPVDDEG FVVDSQDCEE READWRGRGT SRPITVVGNP
60 70 80 90 100
VLHKECEDVT DFGEEFQQLV ADMFASQRTA EGVGLAANQI GVSKKVFVYD
110 120 130 140 150
CPDDEGVRHV GVVCNPRLVE LPADRRRLDD SNEGCLSVPT AYAPLARPDY
160 170 180 190 200
AEVTGQDEKG NPVKVRGTGY FARCLQHETD HLYGYLYIDR LSKRERKDAL
210
RQMAENEPRY PVVAND
Length:216
Mass (Da):24,184
Last modified:October 1, 2000 - v1
Checksum:i562FDF5C60732AAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939122 Genomic DNA. Translation: CAB94606.1.
RefSeqiNP_629368.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAB94606; CAB94606; CAB94606.
GeneIDi1100662.
KEGGisco:SCO5221.
PATRICi23740294. VBIStrCoe124346_5305.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939122 Genomic DNA. Translation: CAB94606.1.
RefSeqiNP_629368.1. NC_003888.3.

3D structure databases

ProteinModelPortaliQ9K4A0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi100226.SCO5221.

Proteomic databases

PRIDEiQ9K4A0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB94606; CAB94606; CAB94606.
GeneIDi1100662.
KEGGisco:SCO5221.
PATRICi23740294. VBIStrCoe124346_5305.

Phylogenomic databases

eggNOGiCOG0242.
HOGENOMiHOG000243508.
InParanoidiQ9K4A0.
KOiK01462.
OMAiGYLYIDR.
OrthoDBiEOG664CMF.
PhylomeDBiQ9K4A0.

Family and domain databases

Gene3Di3.90.45.10. 1 hit.
HAMAPiMF_00163. Pep_deformylase.
InterProiIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
PANTHERiPTHR10458. PTHR10458. 1 hit.
PfamiPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFiPIRSF004749. Pep_def. 1 hit.
PRINTSiPR01576. PDEFORMYLASE.
SUPFAMiSSF56420. SSF56420. 1 hit.
TIGRFAMsiTIGR00079. pept_deformyl. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.

Entry informationi

Entry nameiDEF4_STRCO
AccessioniPrimary (citable) accession number: Q9K4A0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: October 1, 2000
Last modified: January 7, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.