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Protein

Epi-isozizaene synthase

Gene

cyc1

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the cyclization of farnesyl diphosphate (FPP) to the sesquiterpene epi-isozizaene.1 Publication

Catalytic activityi

(2E,6E)-farnesyl diphosphate = (+)-epi-isozizaene + diphosphate.

Cofactori

Mg2+1 Publication, Mn2+1 Publication, Fe3+1 PublicationNote: Binds 3 Mg2+ ions per subunit. Can also use Mn2+ and Fe3+ but less efficiently, and other divalent cations such as Zn2+, Fe2+, Co2+, Cu2+ and Ni2+ are nearly completely inefficient as cofactors.1 Publication

Kineticsi

  1. KM=147 nM for FPP1 Publication

    Pathwayi: epi-isozizaene biosynthesis

    This protein is involved in the pathway epi-isozizaene biosynthesis, which is part of Sesquiterpene biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway epi-isozizaene biosynthesis and in Sesquiterpene biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi99Magnesium 11
    Metal bindingi99Magnesium 21
    Metal bindingi103Magnesium 11
    Metal bindingi103Magnesium 21
    Metal bindingi240Magnesium 31
    Metal bindingi244Magnesium 31
    Metal bindingi248Magnesium 31

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13904.
    BRENDAi4.2.3.37. 5998.
    SABIO-RKQ9K499.
    UniPathwayiUPA00195.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Epi-isozizaene synthase (EC:4.2.3.37)
    Short name:
    EIZS
    Alternative name(s):
    Sesquiterpene cyclase
    Sesquiterpene synthase
    Gene namesi
    Name:cyc1
    Ordered Locus Names:SCO5222
    ORF Names:SC7E4.19
    OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
    Taxonomic identifieri100226 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
    Proteomesi
    • UP000001973 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002478941 – 361Epi-isozizaene synthaseAdd BLAST361

    Interactioni

    Protein-protein interaction databases

    STRINGi100226.SCO5222.

    Structurei

    Secondary structure

    1361
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Turni19 – 21Combined sources3
    Helixi24 – 29Combined sources6
    Helixi38 – 51Combined sources14
    Helixi57 – 67Combined sources11
    Helixi69 – 74Combined sources6
    Helixi82 – 107Combined sources26
    Helixi111 – 126Combined sources16
    Helixi129 – 131Combined sources3
    Helixi137 – 149Combined sources13
    Helixi155 – 181Combined sources27
    Helixi187 – 197Combined sources11
    Helixi200 – 211Combined sources12
    Helixi217 – 220Combined sources4
    Helixi223 – 250Combined sources28
    Beta strandi251 – 254Combined sources4
    Helixi257 – 264Combined sources8
    Helixi269 – 301Combined sources33
    Beta strandi303 – 305Combined sources3
    Helixi306 – 335Combined sources30
    Helixi337 – 339Combined sources3
    Helixi341 – 343Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3KB9X-ray1.60A2-361[»]
    3KBKX-ray1.90A2-361[»]
    3LG5X-ray1.64A1-361[»]
    3LGKX-ray1.89A2-361[»]
    4LTVX-ray2.40A2-361[»]
    4LTZX-ray2.45A2-361[»]
    4LUUX-ray1.95A2-361[»]
    4LXWX-ray2.09A2-361[»]
    4LZ0X-ray1.75A2-361[»]
    4LZ3X-ray2.10A2-361[»]
    4LZCX-ray2.46A2-361[»]
    ProteinModelPortaliQ9K499.
    SMRiQ9K499.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9K499.

    Family & Domainsi

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi99 – 103DDXXD motif5

    Domaini

    The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

    Sequence similaritiesi

    Belongs to the terpene synthase family.Curated

    Phylogenomic databases

    HOGENOMiHOG000250105.
    InParanoidiQ9K499.
    KOiK10156.
    OMAiHESGRYM.
    OrthoDBiPOG091H0EGW.

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    InterProiIPR008949. Isoprenoid_synthase_dom.
    [Graphical view]
    SUPFAMiSSF48576. SSF48576. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9K499-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MHAFPHGTTA TPTAIAVPPS LRLPVIEAAF PRQLHPYWPK LQETTRTWLL
    60 70 80 90 100
    EKRLMPADKV EEYADGLCYT DLMAGYYLGA PDEVLQAIAD YSAWFFVWDD
    110 120 130 140 150
    RHDRDIVHGR AGAWRRLRGL LHTALDSPGD HLHHEDTLVA GFADSVRRLY
    160 170 180 190 200
    AFLPATWNAR FARHFHTVIE AYDREFHNRT RGIVPGVEEY LELRRLTFAH
    210 220 230 240 250
    WIWTDLLEPS SGCELPDAVR KHPAYRRAAL LSQEFAAWYN DLCSLPKEIA
    260 270 280 290 300
    GDEVHNLGIS LITHHSLTLE EAIGEVRRRV EECITEFLAV ERDALRFADE
    310 320 330 340 350
    LADGTVRGKE LSGAVRANVG NMRNWFSSVY WFHHESGRYM VDSWDDRSTP
    360
    PYVNNEAAGE K
    Length:361
    Mass (Da):41,388
    Last modified:October 1, 2000 - v1
    Checksum:i227B08D792F36BB7
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL939123 Genomic DNA. Translation: CAB94607.1.
    RefSeqiNP_629369.1. NC_003888.3.
    WP_011030119.1. NC_003888.3.

    Genome annotation databases

    EnsemblBacteriaiCAB94607; CAB94607; CAB94607.
    GeneIDi1100663.
    KEGGisco:SCO5222.
    PATRICi23740296. VBIStrCoe124346_5306.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL939123 Genomic DNA. Translation: CAB94607.1.
    RefSeqiNP_629369.1. NC_003888.3.
    WP_011030119.1. NC_003888.3.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3KB9X-ray1.60A2-361[»]
    3KBKX-ray1.90A2-361[»]
    3LG5X-ray1.64A1-361[»]
    3LGKX-ray1.89A2-361[»]
    4LTVX-ray2.40A2-361[»]
    4LTZX-ray2.45A2-361[»]
    4LUUX-ray1.95A2-361[»]
    4LXWX-ray2.09A2-361[»]
    4LZ0X-ray1.75A2-361[»]
    4LZ3X-ray2.10A2-361[»]
    4LZCX-ray2.46A2-361[»]
    ProteinModelPortaliQ9K499.
    SMRiQ9K499.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi100226.SCO5222.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAB94607; CAB94607; CAB94607.
    GeneIDi1100663.
    KEGGisco:SCO5222.
    PATRICi23740296. VBIStrCoe124346_5306.

    Phylogenomic databases

    HOGENOMiHOG000250105.
    InParanoidiQ9K499.
    KOiK10156.
    OMAiHESGRYM.
    OrthoDBiPOG091H0EGW.

    Enzyme and pathway databases

    UniPathwayiUPA00195.
    BioCyciMetaCyc:MONOMER-13904.
    BRENDAi4.2.3.37. 5998.
    SABIO-RKQ9K499.

    Miscellaneous databases

    EvolutionaryTraceiQ9K499.

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    InterProiIPR008949. Isoprenoid_synthase_dom.
    [Graphical view]
    SUPFAMiSSF48576. SSF48576. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCYC1_STRCO
    AccessioniPrimary (citable) accession number: Q9K499
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2006
    Last sequence update: October 1, 2000
    Last modified: November 2, 2016
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.