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Protein

Epi-isozizaene synthase

Gene

cyc1

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the cyclization of farnesyl diphosphate (FPP) to the sesquiterpene epi-isozizaene.1 Publication

Catalytic activityi

(2E,6E)-farnesyl diphosphate = (+)-epi-isozizaene + diphosphate.

Cofactori

Mg2+1 Publication, Mn2+1 Publication, Fe3+1 PublicationNote: Binds 3 Mg2+ ions per subunit. Can also use Mn2+ and Fe3+ but less efficiently, and other divalent cations such as Zn2+, Fe2+, Co2+, Cu2+ and Ni2+ are nearly completely inefficient as cofactors.1 Publication

Kineticsi

  1. KM=147 nM for FPP1 Publication

    Pathwayi: epi-isozizaene biosynthesis

    This protein is involved in the pathway epi-isozizaene biosynthesis, which is part of Sesquiterpene biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway epi-isozizaene biosynthesis and in Sesquiterpene biosynthesis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi99 – 991Magnesium 1
    Metal bindingi99 – 991Magnesium 2
    Metal bindingi103 – 1031Magnesium 1
    Metal bindingi103 – 1031Magnesium 2
    Metal bindingi240 – 2401Magnesium 3
    Metal bindingi244 – 2441Magnesium 3
    Metal bindingi248 – 2481Magnesium 3

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13904.
    BRENDAi4.2.3.37. 5998.
    SABIO-RKQ9K499.
    UniPathwayiUPA00195.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Epi-isozizaene synthase (EC:4.2.3.37)
    Short name:
    EIZS
    Alternative name(s):
    Sesquiterpene cyclase
    Sesquiterpene synthase
    Gene namesi
    Name:cyc1
    Ordered Locus Names:SCO5222
    ORF Names:SC7E4.19
    OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
    Taxonomic identifieri100226 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
    Proteomesi
    • UP000001973 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 361361Epi-isozizaene synthasePRO_0000247894Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi100226.SCO5222.

    Structurei

    Secondary structure

    1
    361
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni19 – 213Combined sources
    Helixi24 – 296Combined sources
    Helixi38 – 5114Combined sources
    Helixi57 – 6711Combined sources
    Helixi69 – 746Combined sources
    Helixi82 – 10726Combined sources
    Helixi111 – 12616Combined sources
    Helixi129 – 1313Combined sources
    Helixi137 – 14913Combined sources
    Helixi155 – 18127Combined sources
    Helixi187 – 19711Combined sources
    Helixi200 – 21112Combined sources
    Helixi217 – 2204Combined sources
    Helixi223 – 25028Combined sources
    Beta strandi251 – 2544Combined sources
    Helixi257 – 2648Combined sources
    Helixi269 – 30133Combined sources
    Beta strandi303 – 3053Combined sources
    Helixi306 – 33530Combined sources
    Helixi337 – 3393Combined sources
    Helixi341 – 3433Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3KB9X-ray1.60A2-361[»]
    3KBKX-ray1.90A2-361[»]
    3LG5X-ray1.64A1-361[»]
    3LGKX-ray1.89A2-361[»]
    4LTVX-ray2.40A2-361[»]
    4LTZX-ray2.45A2-361[»]
    4LUUX-ray1.95A2-361[»]
    4LXWX-ray2.09A2-361[»]
    4LZ0X-ray1.75A2-361[»]
    4LZ3X-ray2.10A2-361[»]
    4LZCX-ray2.46A2-361[»]
    ProteinModelPortaliQ9K499.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9K499.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi99 – 1035DDXXD motif

    Domaini

    The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

    Sequence similaritiesi

    Belongs to the terpene synthase family.Curated

    Phylogenomic databases

    HOGENOMiHOG000250105.
    InParanoidiQ9K499.
    KOiK10156.
    OMAiHESGRYM.
    OrthoDBiPOG091H0EGW.

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    InterProiIPR008949. Isoprenoid_synthase_dom.
    [Graphical view]
    SUPFAMiSSF48576. SSF48576. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9K499-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MHAFPHGTTA TPTAIAVPPS LRLPVIEAAF PRQLHPYWPK LQETTRTWLL
    60 70 80 90 100
    EKRLMPADKV EEYADGLCYT DLMAGYYLGA PDEVLQAIAD YSAWFFVWDD
    110 120 130 140 150
    RHDRDIVHGR AGAWRRLRGL LHTALDSPGD HLHHEDTLVA GFADSVRRLY
    160 170 180 190 200
    AFLPATWNAR FARHFHTVIE AYDREFHNRT RGIVPGVEEY LELRRLTFAH
    210 220 230 240 250
    WIWTDLLEPS SGCELPDAVR KHPAYRRAAL LSQEFAAWYN DLCSLPKEIA
    260 270 280 290 300
    GDEVHNLGIS LITHHSLTLE EAIGEVRRRV EECITEFLAV ERDALRFADE
    310 320 330 340 350
    LADGTVRGKE LSGAVRANVG NMRNWFSSVY WFHHESGRYM VDSWDDRSTP
    360
    PYVNNEAAGE K
    Length:361
    Mass (Da):41,388
    Last modified:October 1, 2000 - v1
    Checksum:i227B08D792F36BB7
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL939123 Genomic DNA. Translation: CAB94607.1.
    RefSeqiNP_629369.1. NC_003888.3.
    WP_011030119.1. NC_003888.3.

    Genome annotation databases

    EnsemblBacteriaiCAB94607; CAB94607; CAB94607.
    GeneIDi1100663.
    KEGGisco:SCO5222.
    PATRICi23740296. VBIStrCoe124346_5306.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL939123 Genomic DNA. Translation: CAB94607.1.
    RefSeqiNP_629369.1. NC_003888.3.
    WP_011030119.1. NC_003888.3.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3KB9X-ray1.60A2-361[»]
    3KBKX-ray1.90A2-361[»]
    3LG5X-ray1.64A1-361[»]
    3LGKX-ray1.89A2-361[»]
    4LTVX-ray2.40A2-361[»]
    4LTZX-ray2.45A2-361[»]
    4LUUX-ray1.95A2-361[»]
    4LXWX-ray2.09A2-361[»]
    4LZ0X-ray1.75A2-361[»]
    4LZ3X-ray2.10A2-361[»]
    4LZCX-ray2.46A2-361[»]
    ProteinModelPortaliQ9K499.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi100226.SCO5222.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAB94607; CAB94607; CAB94607.
    GeneIDi1100663.
    KEGGisco:SCO5222.
    PATRICi23740296. VBIStrCoe124346_5306.

    Phylogenomic databases

    HOGENOMiHOG000250105.
    InParanoidiQ9K499.
    KOiK10156.
    OMAiHESGRYM.
    OrthoDBiPOG091H0EGW.

    Enzyme and pathway databases

    UniPathwayiUPA00195.
    BioCyciMetaCyc:MONOMER-13904.
    BRENDAi4.2.3.37. 5998.
    SABIO-RKQ9K499.

    Miscellaneous databases

    EvolutionaryTraceiQ9K499.

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    InterProiIPR008949. Isoprenoid_synthase_dom.
    [Graphical view]
    SUPFAMiSSF48576. SSF48576. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCYC1_STRCO
    AccessioniPrimary (citable) accession number: Q9K499
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2006
    Last sequence update: October 1, 2000
    Last modified: September 7, 2016
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.